Detection of actomyosin depolymerization with a piezoelectric quartz crystal

Kurosawa, Shigeru; Nemoto, Eiji; Muratsugu, Makoto; Yoshimoto, Minoru; Mori, Yoshihito; Kamo, Naoki

doi:10.1016/0003-2670(94)90006-v

Cited by 6 publications

(5 citation statements)

References 23 publications

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“…The real-time monitoring of the QCM and QCM-D is ideal for such assays as they are able to measure changes in binding state and viscoelastic properties as they occur. As early as 1994, researchers analyzed reconstituted in vitro assays of actomyosin depolymerization using piezoelectric quartz crystals (Kurosawa et al, 1994). While…”

Section: Qcm Studies Of Reconstituted Cytoskeletal Assembliesmentioning

confidence: 99%

“…The real‐time monitoring of the QCM and QCM‐D is ideal for such assays as they are able to measure changes in binding state and viscoelastic properties as they occur. As early as 1994, researchers analyzed reconstituted in vitro assays of actomyosin depolymerization using piezoelectric quartz crystals (Kurosawa et al, 1994). While Kurosawa et al did not use a QCM, the same principle of analyzing changes in the resonance frequency of a quartz crystal was present in their study (Kurosawa et al, 1994).…”

Section: Qcm Studies Of Reconstituted Cytoskeletal Assembliesmentioning

confidence: 99%

“…As early as 1994, researchers analyzed reconstituted in vitro assays of actomyosin depolymerization using piezoelectric quartz crystals (Kurosawa et al, 1994). While Kurosawa et al did not use a QCM, the same principle of analyzing changes in the resonance frequency of a quartz crystal was present in their study (Kurosawa et al, 1994). As this was prior to the development of the QCM‐D, the authors alternatively used a viscometer to determine the viscous character of their actomyosin solution (Kurosawa et al, 1994).…”

Section: Qcm Studies Of Reconstituted Cytoskeletal Assembliesmentioning

confidence: 99%

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Molecular and cellular level characterization of cytoskeletal mechanics using a quartz crystal microbalance

et al. 2023

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A quartz crystal microbalance (QCM) is an instrument that has the ability to measure nanogram‐level changes in mass on a quartz sensor and is traditionally used to probe surface interactions and assembly kinetics of synthetic systems. The addition of dissipation monitoring (QCM‐D) facilitates the study of viscoelastic systems, such as those relevant to molecular and cellular mechanics. Due to real‐time recording of frequency and dissipation changes and single protein‐level precision, the QCM‐D is effective in interrogating the viscoelastic properties of cell surfaces and in vitro cellular components. However, few studies focus on the application of this instrument to cytoskeletal systems, whose dynamic parts create interesting emergent mechanics as ensembles that drive essential tasks, such as division and motility. Here, we review the ability of the QCM‐D to characterize key kinetic and mechanical features of the cytoskeleton through in vitro reconstitution and cellular assays and outline how QCM‐D studies can yield insightful mechanical data alone and in tandem with other biophysical characterization techniques.

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Section: Qcm Studies Of Reconstituted Cytoskeletal Assembliesmentioning

confidence: 99%

Section: Qcm Studies Of Reconstituted Cytoskeletal Assembliesmentioning

confidence: 99%

Section: Qcm Studies Of Reconstituted Cytoskeletal Assembliesmentioning

confidence: 99%

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Molecular and cellular level characterization of cytoskeletal mechanics using a quartz crystal microbalance

et al. 2023

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“…A TSM sensor combined with a spectrum analyser was used to monitor the viscosity decrease during the depolymerization of a muscle protein, actomyosin, induced by the addition of adenosine 5A-triphosphate in its solution. 142 On the basis of the experimental results, the authors suggested the further development of a sensor for monitoring the freshness of meat. In another study, the same authors applied the TSM device to monitor the viscosity change of the solution due to an immunoreaction in association with the specific agglutination of streptolysin-bearing latex particles induced by the introduction of lysin.…”

Section: Other Applicationsmentioning

confidence: 99%

Acoustic waves and the study of biochemical macromolecules and cells at the sensor–liquid interface

Čavić¹,

Thompson²,

Hayward³

1999

Analyst

149

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Introduction 2 Overview of acoustic wave devices 3 Acoustic waves and the liquid-solid interface 4 Measurement techniques 5 Protein adsorption 6 Detection of interfacial immunochemical interactions 7 Nucleic acids and DNA/RNA-protein/peptide interactions. Drug discovery 8 Cell adhesion and cell function 9 Other applications 10 Concluding remarks and future perspectives 11 Acknowledgements 12 References I am skeptical of the principle of objectivity, which, in my view, is often simply the current popular viewpoint in disguise.

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“…One of these sensors, the BAW viscosity sensor, is an important type in which the viscosity and density response of the sensor is utilized. This kind of sensor has been successfully applied to many areas, such as determination of endotoxin based on the gelation of Limulus amebocyte lysate [5], detection of ®brinogen based on its gelation with thrombin [6], monitoring of blood coagulation [7], detection of rheumatoid factor [8] and antistreptolysin O antibody by latex piezoelectric immunoassay [9], detection of actomyosin depolymerization [10], monitoring of dextran fermentation [11], piezoelectric pH sensors based on viscoelastic changes in phase transitions of a polymer ®lm [12].…”

mentioning

confidence: 99%

A Bulk Acoustic Wave Viscosity Sensor for Determination of Lysozyme Based on Lysis of Micrococcus Lysodeikeicus

Bao

Chen

et al. 1999

Mikrochim Acta

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A method for determination of lysozyme with a Bulk Acoustic Wave (BAW) viscosity sensor is presented. It is based on the bacteriolytic action of lysozyme on Micrococcus lysodeikeicus (M. lysodeikeicus) and the response of the sensor to the viscosity and density change of this process. There was a good correlation between the frequency shift and the concentration of lysozyme in the range 10±100 mg/ml. The content of lysozyme in human saliva was determined by this method and the results obtained were in good agreement with those from the conventional turbidimetric method. This method has an advantage over the conventional turbidimetric method in that the amount of sample needed is smaller, the procedure is simpler and the concentration range of the bacterium suspension which can be used in the detection was extended.

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Detection of actomyosin depolymerization with a piezoelectric quartz crystal

Cited by 6 publications

References 23 publications

Molecular and cellular level characterization of cytoskeletal mechanics using a quartz crystal microbalance

Molecular and cellular level characterization of cytoskeletal mechanics using a quartz crystal microbalance

Acoustic waves and the study of biochemical macromolecules and cells at the sensor–liquid interface

A Bulk Acoustic Wave Viscosity Sensor for Determination of Lysozyme Based on Lysis of Micrococcus Lysodeikeicus

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