A Bulk Acoustic Wave Viscosity Sensor for Determination of Lysozyme Based on Lysis of Micrococcus Lysodeikeicus

Wei

et al. 2002

Analyst

Self Cite

The effect of magnetic field on the growth of bacteria was studied with the series piezoelectric quartz crystal (SPQC) sensing technique. The growth situations of Escherichia coli (E. coli) in the absence and presence of different intensities of static magnetic fields were examined and analyzed. The results showed that the growth of E. coli was inhibited due to the presence of magnetic fields. By fitting frequency shift (deltaD) versus time curves according to the frequency shift response equation of SPQC, the relationships between three kinetic growth parameters, i.e., the asymptote A, the maximum specific growth rate mu(m) and lag time lambda, and magnetic field intensity were established. Based on these results, a new response model containing the magnetic field intensity was derived as: delta(f) = 167.7 (7.25 - 7.11B)/[1 + exp[4 x 2.46e(-3.97B)/(7.25 -7.1 IB)] x (4.42 + 16.46B - t) + 2]] The kinetic parameters of bacterial growth obtained from this model are close to those obtained from the logistics popular growth model, in which the concentration of the bacteria was determined by the traditional pour plate count method.

Section: Introductionmentioning

confidence: 99%

Effect of static magnetic field on growth of Escherichia coli and relative response model of series piezoelectric quartz crystal

Wei

et al. 2002

Analyst

Self Cite

Materials Chemistry and Physics

“…Aptasensors based on electrochemical interactions have been demonstrated with a high degree of selectivity in the coexistence of other proteins [1,2]. Furthermore complex techniques like electrochemical impedance spectroscopy based on suitable aptamers as molecular recognition element [3], or monitoring of Atomic Force Microscopy -AFM cantilevers' deflection and bending under electrostatic interactions [4], and Bulk Acoustic Wave (BAW) viscosity sensor [5] were used as protein detection methods. Although some of the aforementioned techniques achieve a high degree of sensitivity, their implementation and use are rather laborious, expensive and require complex interrogation instrumentation setups leading to time consuming processes that in many cases are inapplicable in practical applications.…”

Section: Introductionmentioning

confidence: 99%

Development of amphiphilic block copolymers as silica optical fiber overlayers for BSA protein detection

Petropoulou

Gibson²,

Themistou³

et al. 2018

Development of amphiphilic block copolymers as silica optical fiber overlayers for BSA protein detection, (2018), doi: 10.1016/j. Materials Chemistry and Physics matchemphys.2018.06.027 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

“…Piezoelectric devices, such as bulk acoustic wave (BAW) or surface acoustic wave (SAW) devices, have started to be used successfully for biosensing purposes. In fact, they exhibit high sensitivity in mass [ 1 , 2 ], in viscous/viscoelastic changes [ 3 , 4 ], as well as in the detection of conformational changes of surface-bound biomolecules for better understanding of biomolecular interactions [ 5 , 6 ]. In the field of biosensors, the major strengths of the resonant piezoelectric devices are their high sensitivity and versatility and their capacity to yield wireless and portable devices of very small size.…”

Section: Introductionmentioning

confidence: 99%

A Fluidic Interface with High Flow Uniformity for Reusable Large Area Resonant Biosensors

et al. 2017

Resonant biosensors are known for their high accuracy and high level of miniaturization. However, their fabrication costs prevent them from being used as disposable sensors and their effective commercial success will depend on their ability to be reused repeatedly. Accordingly, all the parts of the sensor in contact with the fluid need to tolerate the regenerative process which uses different chemicals (H3PO4, H2SO4 based baths) without degrading the characteristics of the sensor. In this paper, we propose a fluidic interface that can meet these requirements, and control the liquid flow uniformity at the surface of the vibrating area. We study different inlet and outlet channel configurations, estimating their performance using numerical simulations based on finite element method (FEM). The interfaces were fabricated using wet chemical etching on Si, which has all the desirable characteristics for a reusable biosensor circuit. Using a glass cover, we could observe the circulation of liquid near the active surface, and by using micro-particle image velocimetry (μPIV) on large surface area we could verify experimentally the effectiveness of the different designs and compare with simulation results.