Detection and Whole-Genome Characterization of a G8P[1] Group A Rotavirus Strain from Deer

Anbalagan, Srivishnupriya; Peterson, Jessica

doi:10.1128/genomea.01339-16

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…Phylogenetic analyses of partial VP4 gene of nucleotide and deduced amino acid sequences of P [1] have been reported, indicating the prevalence of P types of different reassortants in animal host species in Japan, United States, South America and most of countries of Europe [3,12,13,25]. The P [5] genotype of bovine RVA sequences also clustered with the P[5]-VIII lineage, and clustered with bovine-derived human CJN-M and RotaTeq vaccine strains [30,33].…”

Section: Discussionmentioning

confidence: 94%

G and P genotype profiles of rotavirus a field strains circulating in a vaccinated bovine farm as parameters for assessing biosecurity level

Hasan

Kabir

Miyaoka

et al. 2022

The Journal of Veterinary Medical Science

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After improvement of hygiene protocols on boots in a bovine operation (farm A) in Ibaraki, Japan in September 2017, mortality of calves and the detection of 4 viral pathogen indicators, including bovine rotavirus A (RVA), became significantly low for one year. Subsequently, in the present study, these indicators and mortality were monitored and confirmed all were still low, except for the detection rate of bovine RVA in calves less than 3 weeks old. The present study aimed to investigate G and P genotypic profiles of RVAs in farm A from 2018 to 2020.Molecular analysis using semi-nested multiplex RT-PCR of positive RVAs (n = 122) and sequencing of selected samples revealed the presence of G6, G8, G10, P[1], P[5] and P [11] genotypes and the prevalence of G and/or P combination and mixed infections. The most common combination of G and P types was G10P[11] (41.8%), followed by mixed infection with G6+G10P[5] (11.5%). Phylogenetic analysis of RVAs showed clustering with bovine and other animal-derived RVA strains, suggesting the possibility of multiple reassortant events with strains of bovine and others animal origins. Noteworthy as well is that vaccinated cattle might fail to provide their offspring with maternal immunity against RVA infections, due to insufficient colostrum feeding. Our findings further highlight the importance of RVA surveillance in bovine populations, which may be useful to improving effective routine vaccination and hygiene practices on bovine farms.

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Section: Discussionmentioning

confidence: 94%

G and P genotype profiles of rotavirus a field strains circulating in a vaccinated bovine farm as parameters for assessing biosecurity level

Hasan

Kabir

Miyaoka

et al. 2022

The Journal of Veterinary Medical Science

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“…In fact, only a few data has been published on the distribution of RVA in wild ungulates, including species frequently hunted and serving as food. So far, the presence of RVA has been described in wild boars from Japan, the Czech Republic, and Croatia [ 9 , 20 , 21 ], in roe deer from Slovenia [ 22 , 23 ], in water deer from South Korea [ 24 ], as well as in two unspecified deer samples [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Rotaviruses in Wild Ungulates from Germany, 2019–2022

2023

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Rotavirus A (RVA) is an important cause of diarrhea in humans and animals. However, RVA in wild animals has only scarcely been investigated so far. Here, the presence of RVA in wild ungulates hunted between 2019 and 2022 in Brandenburg, Germany, was investigated using real-time RT-PCR and sequencing of RT-PCR products. By analyzing intestinal contents, RVA-RNA was detected in 1.0% (2/197) of wild boar (Sus scrofa), 1.3% (2/152) of roe deer (Capreolus capreolus), and 2.1% (2/95) of fallow deer (Dama dama) but not in 28 red deer (Cervus elaphus) samples. Genotyping identified G3P[13] strains in wild boar, which were closely related to previously described pig and wild boar strains. Genotype G10P[15] strains, closely related to strains from roe deer, sheep, or cattle, were found in roe deer. The strains of fallow deer represented genotype G3P[3], clustering in a group containing different strains from several hosts. The results indicated a low prevalence of RVA in wild ungulates in Germany. Associations of specific genotypes with certain ungulate species seem to exist but should be confirmed by analyses of more samples in the future.

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Whole genome sequence and a phylogenetic analysis of the G8P[14] group A rotavirus strain from roe deer

et al. 2017

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BackgroundGroup A rotaviruses (RVA) are associated with acute gastroenteritis in children and in young domestic and wild animals. A RVA strain was detected from a roe deer for the first time during a survey of game animals in Slovenia in 2014. A further RVA strain (SLO/D110–15) was detected from a roe deer during 2015. The aim of this study was to provide a full genetic profile of the detected RVA strain from roe deer and to obtain additional information about zoonotic transmitted strains and potential reassortments between human rotavirus strains and zoonotic transmitted rotavirus strains. The next generation sequencing (NGS) analysis on Ion Torrent was performed and the whole genome sequence has been determined together with a phylogenetic analysis.ResultsThe whole genome sequence of SLO/D110–15 was obtained by NGS analyses on an IonTorrent platform. According to the genetic profile, the strain SLO/D110–15 clusters with the DS-1-like group and expresses the G8-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3 genome constellation. Phylogenetic analysis shows that this roe deer G8P[14] strain is most closely related to RVA strains found in sheep, cattle and humans. A human RVA strain with the same genotype profile was detected in 2009 in Slovenia.ConclusionsThe G8P[14] genotype has been found, for the first time, in deer, a newly described host from the order Artiodactyla for this RVA genotype. The finding of a rotavirus with the same genome segment constellation in humans indicates the possible zoonotic potential of this virus strain.

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Detection and Whole-Genome Characterization of a G8P[1] Group A Rotavirus Strain from Deer

Cited by 3 publications

References 5 publications

G and P genotype profiles of rotavirus a field strains circulating in a vaccinated bovine farm as parameters for assessing biosecurity level

G and P genotype profiles of rotavirus a field strains circulating in a vaccinated bovine farm as parameters for assessing biosecurity level

Rotaviruses in Wild Ungulates from Germany, 2019–2022

Whole genome sequence and a phylogenetic analysis of the G8P[14] group A rotavirus strain from roe deer

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