Detection and sequencing of Potato virus Y (PVY) and Potato leafroll virus (PLRV) in a volunteer plant of Solanum tuberosum L. cv. Diacol-Capiro

Abstract

Viral diseases are among the most limiting factors in the production of potato in Colombia and the rest of the world. The best strategy to control plant viruses consists on the use of certified seed tubers, control of arthropod vectors and the use of adequate crop management practices that reduce mechanical transmission and the presence of viral reservoirs like weeds and volunteer plants. However, the successful implementation of these practices relies on the availability of highly sensitive techniques that allow for the asymptomatic detection of viruses. In this work, we tested the performance of Next-generation sequencing (NGS) and real time RT-PCR (RT-qPCR) on a single volunteer potato plant (cv. Diacol-Capiro) growing naturally in a seed-tuber storage facility in Yarumal (Antioquia). Our NGS results demonstrate a mixed infection with Potato virus Y (PVY) and Potato leafroll virus (PLRV). RT-qPCR was performed in roots, main stolons, crown (root collar) and upper, middle and lower leaves using specific primers for PVY, PLRV, Potato virus S (PVS), Potato virus V (PVV), Potato virus X (PVX) and Potato yellow vein virus (PYVV). Only PVY and PLRV were detected in good agreement with the NGS data. This work demonstrates the usefulness of both techniques for supporting integrated management of plant viruses in potato, including virus detection in natural reservoirs such as volunteer plants and weeds.

Insight into aphid mediated Potato Virus Y transmission: A molecular to bioinformatics prospective

“…1. Adopting a suitable diagnostic tool for proper identification (Glais et al, 2017) can be useful in understanding PVY and their characteristic mode of transmission (by various strains), genome organization and protein function, genetic diversity, molecular and bioinformatics aspects of PVY transmission by aphids, methods used in detection and characterization of PVY (Medina Cárdenas et al, 2017), transcriptomics level of response, identification, and monitoring of virus transmission, and vector control strategies in an exclusive manner. 2.…”

“…Furthermore, a volunteer plant of potato (cv. Diacol-Capiro) was infected with PVY and the potato leaf roll virus (PLRV) (Medina Cárdenas et al, 2017 ). In this study, researchers observed the effectiveness of RT-PCR (RT-qPCR) and next-generation sequencing (NGS).…”

Coinfección natural de virus de ARN en cultivos de papa (<i>Solanum tuberosum</i> subsp. Andigena) en Antioquia (Colombia)

“…En los últimos años, se ha aumentado considerablemente la información sobre los virus que afectan diferentes solanáceas cultivadas en Colombia y están disponibles los genomas completos de cepas de las razas PVY O , PVY N y PVY NTN en papa (Villamil-Garzón et al, 2014;Muñoz et al, 2016;Medina et al, 2017;Riascos et al, 2018); de PYVV en papa criolla, tomate (S. lycopersicum) y lulo (S. quitoense) (Villamil-Garzón et al, 2014;Álvarez et al, 2017;Baena et al, 2017;Gallo et al, 2018); así como también de los linajes PVS A y PVS P en papa criolla (Gutiérrez et al, 2012;Vallejo et al, 2016), de PVX en uchuva (Physalis peruviana) y de PLRV en plantas voluntarias de papa (Medina et al, 2017).…”