Detection and Possible Source of Contaminating Pleuropneumonialike Organisms (PPLO) in Cultures of Tissue Cells

Rothblat, George H.; Morton, Harry E.

doi:10.3181/00379727-100-24533

Cited by 43 publications

(18 citation statements)

References 2 publications

(3 reference statements)

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“…If one tissue culture can be contaminated from another maintained in the same laboratory, the infection of only one cell line could serve as a reservoir for the contamination of others. In view of the failure to find mycoplasma in sera used in tissue culture media (Rothblat & Morton, 1959;Coriell, Fabrizio & Wilson, 1960;Klieneberger-Nobel, unpublished observations) it is unlikely that, as suggested by Herderschee et al (1963), the source of the contaminant was human serum.…”

Section: Discussionmentioning

confidence: 94%

The relationship of a type of Mycoplasma isolated from tissue cultures to a new human oral Mycoplasma

Lemcke

1964

J. Hyg.

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Section: Discussionmentioning

confidence: 94%

The relationship of a type of Mycoplasma isolated from tissue cultures to a new human oral Mycoplasma

Lemcke

1964

J. Hyg.

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“…Some workers (Rothblat & Morton, 1959) have suggested that such contaminants may be L-phases of bacteria. Barile, Malizia & Riggs (1962) reported a much lower frequency of contaminants in tissue cultures from laboratories where cells were maintained mainly in the absence of antibiotics.…”

Section: Discussionmentioning

confidence: 99%

A Mycoplasma which Induces Acidity and Cytopathic Effect in Tissue Culture

Butler

Leach

1964

Journal of General Microbiology

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“…Infexgion.--Since it is common knowledge that many cell lines have been contaminated by L forms or/and pleuropneumonia-like organisms (PPLO) (21) it was essential to provide evidence that the differential response of cells to MEF virus was not attributable to the presence or absence of PPLO. Parent and the $3 strains of HeLa cells obtained from commercial sources were freed of PPLO contaminants by serial cultivation for a period of not less than 4 weeks in standard growth medium containing added (25 /zg./ml.)…”

Section: Effect Of Pplo Contamination On Morphologi¢ Response Of Cellmentioning

confidence: 99%

Differentiation of Closely Related Cells by a Variant of Poliovirus, Type 2, Mef1 Strain

Murphy

Armstrong

1959

The Journal of Experimental Medicine

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PLATES 41 AND 42(Received for publication, June 27, 1959) The conceptual and technical problems associated with latent viral infections have been summarized in recent reviews (1-3) from quite different points of view. Investigation of persistent infection by viruses of mammalian cells in culture has failed to disclose the anticipated occurrence of a lysogenic-like relationship. Numerous studies (4-12) have revealed, instead, a much more complex dynamic equilibrium which permits cells and virus to multiply simultaneously. Since it may not be too fruitful to insist that a lysogenic relationship must constitute a phase of mammalian cell-virus interaction, future experimental design should be more profitable if it takes into accord the multiple phenomena known to be operative. Although substantial progress has been made in establishing some of the factors that determine the nature of cellular susceptibility or resistance to mammalian virus infection (13), its applicability to the "non-cytopathogenic" virus-cell relationship has not been evaluated.Thus, in the case of "moderate" or "temperate" mammalian viruses the basis for the nature of cellular resistance to viral infection and ensuing cytopathogenicity has not been characterized within the framework of classical concepts (14).Experiments which provided an experimental system that appeared to be satisfactory for such studies (15) are the subject of this report which describes the interaction between a variant of pollovirus, Type 2, MEFx strain and a spectrum of primate cell lines. The reaction between this virus and HeLa cells appears to be particularly suitable because of the: (a) relative thermal stability of virus; (b) sharp differential cytopathogenicity of virus for closely related cells; (c) availability of a number of genetic markers for virus including animal and cell culture hosts; (d) simultaneous serial propagation of both virus and cells without resort to the protective effect of homotypic antiserum; (e) observed stability of the virus-cell relationship over a period of 4 years (16).

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Detection and Possible Source of Contaminating Pleuropneumonialike Organisms (PPLO) in Cultures of Tissue Cells

Cited by 43 publications

References 2 publications

The relationship of a type of Mycoplasma isolated from tissue cultures to a new human oral Mycoplasma

The relationship of a type of Mycoplasma isolated from tissue cultures to a new human oral Mycoplasma

A Mycoplasma which Induces Acidity and Cytopathic Effect in Tissue Culture

Differentiation of Closely Related Cells by a Variant of Poliovirus, Type 2, Mef1 Strain

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