A Mycoplasma which Induces Acidity and Cytopathic Effect in Tissue Culture

Butler, Michael; Leach, R. H.

doi:10.1099/00221287-34-2-285

Cited by 72 publications

(25 citation statements)

References 27 publications

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“…Isolated from tissue cultures inoculated with human leukaemic material. Subsequently identified $ Isolated from uninoculated HEp-2 cells (Butler & Leach, 1964) and identified as M. hyorhinis as strain of M. puZmonis (Fallon et al 1965; Leach & Butler, 1966 …”

Section: Introductionmentioning

confidence: 99%

Haemadsorption and Haemagglutination by Mycoplasmas

Manchee¹,

Taylor‐Robinson²

1968

Journal of General Microbiology

165

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SUMMARYFactors concerned in demonstrating haemadsorption and haemagglutination and their occurrence among different mycoplasmas were investigated. Haemadsorption occurred best to colonies which had recently developed on agar at pH 6.5. Mycoplasmas isolated from various bird and animal sources, e.g. Mycoplasma gallisepticum, M. agalactiae, M. bovigenitalium and M . pulmonis, haemadsorbed with erythrocytes from a wide range of species. However, not all strains within a serotype haemadsorbed. Thus, the 'Negroni' strain of M . pulmonis did not. Haemadsorption could be inhibited by crowding of colonies on agar and by the addition of specific antiserum to the colonies. Generally, antiserum titres obtained by haemadsorption inhibition were low in comparison with those obtained by metabolic inhibition, and haemadsorption inhibition was not useful as a routine serological technique.The development of the haemagglutinin of Mycoplasma gallisepticum in liquid medium was studied in detail; a change in the pH value of the medium could be used as an index of its development and it was intimately associated with the organism. The centrifuged deposits of other mycoplasmas, from birds, cattle, goats, man, rodents and pigs, and which were grown in liquid medium also haemagglutinated, but generally to low titre. Haemagglutination occurred best in U-shaped cups at 37' and at pH 6.5-7-0 and could be inhibited by specific antiserum. There was lack of correlation between haemadsorption and haemagglutination; both these phenomena were exhibited by some mycoplasmas, others haemadsorbed only, and still others haemagglutinated only.

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Section: Introductionmentioning

confidence: 99%

Haemadsorption and Haemagglutination by Mycoplasmas

Manchee¹,

Taylor‐Robinson²

1968

Journal of General Microbiology

165

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“…)- (Robinson, Wichelhausen & Roizman, 1956;Rothblat, 1960;Pollock, Kenny & Syverton, 1960;Carski & Shepard, 1961), although it may result in depression of the growth of the tissue-culture cells (Kenny & Pollock, 1963); or (b) with the production of a visible c.p.e. of varying type and degree (Hayfbck & Stinebring, 1960;Nelson, 1960;Casterjon-Diez, Fisher & Fisher, 1963 ;ROVOZZO, Luginbuhl & Helmboldt, 1963;Grumbles, Hall & Cummings, 1964;Butler & Leach, 1964). During the course of investigation of outbreaks of bovine infertility strain M 120 of Mycoplasma bovigenitalium was isolated from vaginal swabs obtained from a heifer showing lesions of granular vulvovaginitis (Afshar, Stuart & Huck, 1966).…”

Section: Introductionmentioning

confidence: 99%

The Growth of Mycoplasma bovigenitalium in Cell Cultures

Afshar

1967

Journal of General Microbiology

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SUMMARYA strain of Mycoplasma bovigenitalium, designated M 120, was grown, and produced a marked cytopathic effect (c.p.e.) in calf-, pig-, and monkeykidney cell cultures. The c.p.e. was characterized by enlargement of the cells, the appearance of intracytoplasmic inclusions and partial destruction of the cell monolayers. A similar c.p.e. was produced in tissue-culture cells following the inoculation of mycoplasma 'toxins'.Comparative growth studies of strain M 120 in calf-kidney cell cultures and in tissue-culture medium alone showed that the organism grew more readily in the presence of cells. The rate of virus production and appearance of c.p.e. of infectious bovine rhinotracheitis (I.B.R.) virus was delayed in cultures previously infected with the M 120 strain of mycoplasma as compared with normal cultures.

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“…Mycoplasma species. Seed cultures of the following Mycoplasma species were supplied by R. M. Chanock: M. pneumoniae (FH-Liu), M. hominis (PG-21), M. salivarium (PG-20), M. fermentans (PG-18), M. arthritidis (PG-27), M. orale type I (CH-19299), M. orale type II (CH-20247), Mycoplasma strain GDL (2,12), and Mycoplasma strain S-880 (7,11). The Mycoplasma strain herein designated as MaBy was isolated by one of us (R. A. D.) from the sputum of a pneumonia patient in Pittsburgh in October 1964.…”

Section: Methodsmentioning

confidence: 99%

Immunofluorescence Identification ofMycoplasmaon Agar by Use of Incident Illumination

Giudice¹,

Robillard²,

Carski³

1967

J Bacteriol

190

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The fluorescent-antibody method has been employed for the rapid identification of Mycoplasma colonies growing on agar plates. The method was found to be effective for detection of mixtures of Mycoplasma serotypes growing on primary isolation plates. The technique also helped to define the presence of mycoplasmas which did not produce typical colonies. It was also possible to identify Mycoplasma colonies overgrown by bacterial or fungal contaminants. Conjugates directed against 10 distinct Mycoplasma serotypes have been successfully employed in this system. One of the serotypes is a human oral isolate which has not been previously characterized. The definitive identification of mycoplasmas requires antigenic analysis. A number of techniques have been employed for this purpose. Complement fixation, agglutination, indirect hemagglutination, metabolic inhibition, and growth inhibition have all been used to define the antigenic relationships of mycoplasmas, but these techniques do not allow prompt serotyping of new isolates. The common occurrence of mixed cultures of different serotypes makes it necessary to clone all cultures and use a number of single colonies for the preparation of large stocks of antigen. Employing the standard procedure ofcloning three to five colonies, there is little assurance that all strains in a mixed culture will be detected. These

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A Mycoplasma which Induces Acidity and Cytopathic Effect in Tissue Culture

Cited by 72 publications

References 27 publications

Haemadsorption and Haemagglutination by Mycoplasmas

Haemadsorption and Haemagglutination by Mycoplasmas

The Growth of Mycoplasma bovigenitalium in Cell Cultures

Immunofluorescence Identification ofMycoplasmaon Agar by Use of Incident Illumination

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