Detection and modeling of anti-Leptospira IgG prevalence in cats from Lisbon area and its correlation to retroviral infections, lifestyle, clinical and hematologic changes

Silva, Joana Moreira da; Prata, Sara; Domingues, Tiago Dias; Leal, Rodolfo Oliveira; Nunes, Teresa; Tavares, Luís; Almeida, Virgílio; Sepúlveda, Nuno; Gil, Solange

doi:10.1016/j.vas.2020.100144

Cited by 12 publications

(9 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, a high cutoff for the data might not define seropositivity per se , but rather a high antibody response whose detection could be the primary objective of the analysis ( 30 , 31 ). The use of a high cutoff is also in accordance with a modeling approach where seropositivity might be subdivided into different levels ( 60 – 62 ). Therefore, our sensitivity-like approach seems to have the capacity to detect further serological associations beyond the ones based on the classical seroprevalence.…”

Section: Discussionmentioning

confidence: 99%

Herpesviruses Serology Distinguishes Different Subgroups of Patients From the United Kingdom Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Biobank

et al. 2021

Self Cite

View full text Add to dashboard Cite

The evidence of an association between Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) and chronic herpesviruses infections remains inconclusive. Two reasons for the lack of consistent evidence are the large heterogeneity of the patients' population with different disease triggers and the use of arbitrary cutoffs for defining seropositivity. In this work we re-analyzed previously published serological data related to 7 herpesvirus antigens. Patients with ME/CFS were subdivided into four subgroups related to the disease triggers: S0-42 patients who did not know their disease trigger; S1-43 patients who reported a non-infection trigger; S2-93 patients who reported an infection trigger, but that infection was not confirmed by a lab test; and S3-48 patients who reported an infection trigger and that infection was confirmed by a lab test. In accordance with a sensitivity analysis, the data were compared to those from 99 healthy controls allowing the seropositivity cutoffs to vary within a wide range of possible values. We found a negative association between S1 and seropositivity to Epstein-Barr virus (VCA and EBNA1 antigens) and Varicella-Zoster virus using specific seropositivity cutoff. However, this association was not significant when controlling for multiple testing. We also found that S3 had a lower seroprevalence to the human cytomegalovirus when compared to healthy controls for all cutoffs used for seropositivity and after adjusting for multiple testing using the Benjamini-Hochberg procedure. However, this association did not reach statistical significance when using Benjamini-Yekutieli procedure. In summary, herpesviruses serology could distinguish subgroups of ME/CFS patients according to their disease trigger, but this finding could be eventually affected by the problem of multiple testing.

show abstract

Section: Discussionmentioning

confidence: 99%

Herpesviruses Serology Distinguishes Different Subgroups of Patients From the United Kingdom Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Biobank

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Higher rates of excretion of Leptospira among stray cats could be linked with exposition to higher doses or more frequent exposure due to their environment, contact with stray dogs and predation on small mammals. In addition, their immune system could be compromised and less efficiently control the bacteria carriage due to lower general health or co-infections, with feline immunodeficiency virus for example [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Serological and molecular detection of pathogenic Leptospira in domestic and stray cats on Reunion Island, French Indies

2021

View full text Add to dashboard Cite

Indian Ocean islands are endemic areas for human and animal leptospirosis. Maintenance host species for Leptospira spp. have still not been completely elucidated, and recently the role of cats (Felis catus) has been questioned. This cross-sectional study aims to determine whether cats are part of the maintenance community of different strains of Leptospira spp. in Reunion Island. The prevalence of Leptospira infection in an opportunistic sample of stray and domestic cats (n=92) from Reunion Island has been studied using serological (Microagglutination-Test) and molecular detection (PCR). The results revealed a seroprevalence of 37.0% (34/92) (cut-off 1:40) without a significant difference in the living conditions of animals. The predominant serogroup was Icterohaemorrhagiae, but Ballum, Cynopteri and Australis were also detected. Using PCR, 28.6% (12/42) of stray cats were tested positive. Leptospiral DNA was detected in renal tissue, urine, and blood of respectively 14.3% (6/42), 10.3% (4/39) and 11.9% (5/42) of stray cats, but 0% (0/3), 0% (0/50) and 0% (0/36) of domestic cats (p=non applicable, p=0,038, p=0,058 respectively). Partial rrs gene (16S rRNA) sequencing identified L. interrogans in all PCR positive samples. Our study confirms that renal carriage and urinary shedding are possible, positioning cats, and especially stray cats as potential actors within the maintenance community of L. interrogans in Reunion Island.

show abstract

“…In addition, our study also identified other risks related of leptospirosis to swine or cat raising. The previous studies indicated pigs and cats as reservoirs of Leptospira throughout the world and Vietnam [40][41][42][43][44]. Various serovars, such as Castellonis and Patoc, Tarassovi Mitis, Australis, Javanica, and Autumnalis in swine; and Javanica, Louisiana, Hebdomadis, and Castellonis in cats, were also found in Leptospira patients [21,[45][46][47].…”

Section: Discussionmentioning

confidence: 99%

A case–control study of agricultural and behavioral factors associated with leptospirosis in Vietnam

et al. 2022

View full text Add to dashboard Cite

Introduction Leptospirosis is a neglected disease in Vietnam. Until now, there has been limited knowledge about risk factors of this disease in Vietnam. The study was carried out to identify agricultural and behavioral factors associated with the transmission of leptospirosis in Vietnam. Methods This matched retrospective hospital-community-based case–control study was conducted from 1 October 2018 to 31 October 2019. We recruited cases from 11 selected government hospitals in three provinces of Vietnam, while controls were selected from the same communes of cases and matched by age (± 2 years) and sex. Microscopic agglutination test (MAT) and enzyme-linked immunosorbent assay (ELISA) were applied to determine confirmed cases, while only MAT was used to identify controls with a single high MAT titer < 1:100. Results 504 participants (252 cases and 252 controls) were identified. Cultivating (OR 2.83, CI 1.38–5.79), animal farming (OR 8.26, CI 2.24–30.52), pig owners (OR 10.48, CI 5.05–21.73), cat owners (OR 2.62, CI 1.49–4.61) and drinking unboiled water (OR 1.72, CI 1.14 –2.59, p = 0.010) were significantly associated with human leptospirosis in Vietnam. Hand washing after farming/ gardening (OR 0.57, CI 0.38–0.86, p = 0.007) and bathing after farming, gardening, contact with cattle and poultry (OR 0.33, CI 0.19–0.58, p = 0.000) were determined as protective factors for this disease. Conclusions In short, the case–control study has revealed the risks in agricultural and animal practices and protective behavioral factors related to human leptospirosis in Vietnam. The findings suggested promotion of communication and health education programs targeting health behaviors in daily life and agricultural practices. Using personal protective equipment such as gowns, gloves, and boots during agricultural practices, especially cultivating and animal farming, is most recommended.

show abstract

Detection and modeling of anti-Leptospira IgG prevalence in cats from Lisbon area and its correlation to retroviral infections, lifestyle, clinical and hematologic changes

Cited by 12 publications

References 31 publications

Herpesviruses Serology Distinguishes Different Subgroups of Patients From the United Kingdom Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Biobank

Herpesviruses Serology Distinguishes Different Subgroups of Patients From the United Kingdom Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Biobank

Serological and molecular detection of pathogenic Leptospira in domestic and stray cats on Reunion Island, French Indies

A case–control study of agricultural and behavioral factors associated with leptospirosis in Vietnam

Contact Info

Product

Resources

About