Detection and measurement of cytokines

Thorpe, Robin; Wadhwa, Meenu; Bird, Christopher; Mire-Sluis, AR

doi:10.1016/0268-960x(92)90025-l

Cited by 80 publications

(46 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…17 The MCSF is a hematopoietic growth factor released by the injured endothelium and has the ability to stimulate proliferation, differentiation, and maturation of monocytes and macrophages. 18 In addition, MCSF can stimulate the production of further MCSF from local endothelium and macrophages. 19 Macrophage involvement in cardiac events include various mechanisms such as thrombus organization, smooth muscle cell migration and proliferation, and secretion of proteolytic enzymes.…”

Section: Macrophage Colony Stimulating Factor and Cardiac Eventsmentioning

confidence: 99%

Prognostic Value of C‐Reactive Protein, Fibrinogen, Interleukin‐6, and Macrophage Colony Stimulating Factor in Severe Unstable Angina

Rallidis¹,

Zolindaki²,

Manioudaki³

et al. 2002

Clinical Cardiology

View full text Add to dashboard Cite

SummaryBackground: Inflammatory process plays an important role in the pathogenesis of acute coronary syndromes.Hypothesis: The study was undertaken to evaluate whether admission levels of C-reactive protein (CRP), fibrinogen, interleukin-6 (IL-6), and macrophage colony stimulating factor (MCSF) can predict short-term prognosis in patients with unstable angina.Methods: C-reactive protein, fibrinogen, IL-6, and MCSF were measured on admission in 141 consecutive patients, aged 59 ± 10 years, with unstable angina (Braunwald class IIIb). Patients were divided into two groups according to their in-hospital outcome: Group 1 comprised 77 patients with a complicated course (2 died, 15 developed nonfatal myocardial infarction, and 60 had recurrence of angina), and Group 2 comprised 64 patients with an uneventful course.Results: Admission median levels of CRP (8.8 vs. 3.1 mg/l, p = 0.0002), fibrinogen (392 vs. 340 mg/dl, p = 0.008), .5 pg/ml, p = 0.03), and MCSF (434 vs. 307 pg/ml, p = 0.0001) were higher in Group 1 than in Group 2. The MCSF levels were an independent risk factor for in-hospital events, with an adjusted odds ratio for eventful in-hospital outcome of 3.3 (95% confidence interval 1-10.9, p = 0.04), and correlated with levels of IL-6 (r s = 0.52, p = 0.0001), CRP (rs = 0.43, p = 0.0001), and fibrinogen (rs = 0.25, p = 0.004).Conclusions: These findings suggest that among the studied inflammatory indices only increased admission levels of

show abstract

Section: Macrophage Colony Stimulating Factor and Cardiac Eventsmentioning

confidence: 99%

Prognostic Value of C‐Reactive Protein, Fibrinogen, Interleukin‐6, and Macrophage Colony Stimulating Factor in Severe Unstable Angina

Rallidis¹,

Zolindaki²,

Manioudaki³

et al. 2002

Clinical Cardiology

View full text Add to dashboard Cite

show abstract

“…Particularly the MHC class II alleles, which are mostly responsible for presenting foreign antigen to CD4+ helper T cells, may influence longevity (230) However, it should also be considered that technical differences in experimentation, particularly the measurement of cytokines by immunoassays, could be contributing significantly to the discrepancies in the data obtained by different groups (231). The relatively new technique of intracytoplasmic cytokine staining may help resolve some technical difficulties, because it allows identification of the type of cell secreting the cytokine, at the single cell level.…”

Section: Cytokine Production and Responsementioning

confidence: 99%

T cells and aging update February 1999

Pawelec¹

1999

Front Biosci

View full text Add to dashboard Cite

T cell immunosenescence 218effects of dysregulated immune responses in the elderly by supplementation or other approaches may result in an enhancement of their quality of life, and significant reductions in the cost of medical care in old age. FACTORS CONTRIBUTING TO IMMUNO-SENESCENCE HematopoiesisDysregulated hematopoiesis is seen in elderly individuals, raising the possibility that this could contribute to altered immune function in the aged. Hematopoiesis in man may be compromised because of a severely reduced capacity to produce colony stimulating factors (44) and increased production of pro-inflammatory factors such as IL 6 (45), because lower numbers of progenitor cells are present in the BM (46) and because of an age-related decline in proliferative potential of putative haematopoietic stem cells (HSC) (47). In mice, the repopulating potential of murine fetal liver-derived HSC is higher than that of adult BM-derived stem cells (48), suggesting possible aging of the HSC themselves. Normal cells with shorter telomeres possess less remaining replicative capacity than those with longer telomeres (see section 5.2). Accordingly, HSC from adult BM were found to have shorter telomeres than fetal liver-derived or umbilical cord-derived stem cells, consistent with aging of HSC (49). Telomere lengths decreased on culture despite low level expression of telomerase in these cells (50,51), although the rate of basepair loss per population doubling of cells in culture was lower during the first two weeks, when telomerase was upregulated, than in the next two, when it was downregulated (52). The telomerase expressed is therefore functionally active but may not be able to completely maintain telomere length in aging HSC cells. True embryonic stem cells (ESC), on the other hand, which may really be immortal, do retain high levels of telomerase activity (53). On the other hand, ESC from telomerase-knockout mice show gradual telomere shortening over many population doublings (PD) resulting in slowing and eventual cessation of growth (54). Certain animals which do not downregulate telomerase manifest a permanent growth phase and little or no senescence (55,56).

show abstract

“…These functions include mediation or regulation of immune-responses. 24,25 Since these powerful regulatory and communication molecules are involved in the pathogenesis of disease, there is an increased demand for their detection at clinically relevant concentrations. 25 For example, Graham and co-workers achieved the sensitive detection of tumor necrosis factor a (TNF-a) with resonance Raman (RR) spectroscopy.…”

mentioning

confidence: 99%

Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells

Wang

Salehi

Schütz

et al. 2013

Analyst

View full text Add to dashboard Cite

Rationally designed gold/silver nanoshells (Au/Ag-NS) with plasmon resonances optimized for red laser excitation in order to minimize autofluorescence from clinical samples exhibit scattering cross-sections, which are ca. one order of magnitude larger compared with solid quasi-spherical gold nanoparticles (Au-NPs) of the same size. Hydrophilic stabilization and sterical accessibility for subsequent bioconjugation of Au/Ag-NS is achieved by coating their surface with a self-assembled monolayer (SAM) of rationally designed Raman reporter molecules comprising terminal mono-and tri-ethylene glycol (EG) spacers, respectively. The stability of the hydrophilically stabilized metal colloid was tested under different conditions. In contrast to metal colloids coated with a SAM without terminal EG spacers, the hydrophilically stabilized SERS particles do not aggregate under physiologically relevant conditions, i.e., buffer solutions with high ionic strength. Using these rationally designed SERS particles in conjunction with a microspectroscopic acquisition scheme, a sandwich immunoassay for the sensitive detection of interleukin-6 (IL-6) was developed. Several control experiments demonstrate the high specificity of the assay towards IL-6, with a lowest detectable concentration of ca. 1 pg mL À1 . The signal strength of the Au/Ag-NS is at least one order of magnitude higher compared with hydrophilically stabilized, non-aggregated solid quasi-spherical Au-NPs of the same size.

show abstract

Detection and measurement of cytokines

Cited by 80 publications

References 34 publications

Prognostic Value of C‐Reactive Protein, Fibrinogen, Interleukin‐6, and Macrophage Colony Stimulating Factor in Severe Unstable Angina

Prognostic Value of C‐Reactive Protein, Fibrinogen, Interleukin‐6, and Macrophage Colony Stimulating Factor in Severe Unstable Angina

T cells and aging update February 1999

Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells

Contact Info

Product

Resources

About