Detection and light enhancement of glucose oxidase adsorbed on porous silicon microcavities

Abstract

Porous silicon (PSi) structure is used as support material to detect protein infiltration and to induce fluorescence and second harmonic light enhancement from glucose oxidase (GOX). Functionalization and protein infiltration is monitored by specular reflectometry. Optical response enhancement of PSi microcavity structures compared to PSi single layers or Bragg mirrors is observed, when GOX is impregnated. Penetration of organic molecules along the PSi microcavity structure is demonstrated by energy dispersive X‐ray profile. Enhanced fluorescence emission of GOX when adsorbed on PSi microcavity is evidenced by multi‐photon microscopy (MPM). Second harmonic light generation is observed at some particular pores of PSi and subsequent resonance enhancement of the signal arising from the GOX adsorbed within the pores is detected. Our work evidences an improved device functionality of GOX‐PSi microcavities due to strongly confined and localized light emission within these structures. This opens the way towards the application of PSi microcavity structures as amended biosensors based on their locally enhanced optical response. The second main achievement lies in the novelty of the used techniques. In contrast to the specular reflectometry used to monitor the macroscopic optical response of PSi structures, MPM presents a valuable alternative microscopic technique probing individual pores. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

Development of l-lactate dehydrogenase biosensor based on porous silicon resonant microcavities as fluorescence enhancers

“…3(b) shows that the emission intensity using MC-2 was 10-and 5-fold higher than using single layer and detuned MC, respectively. This is in agreement with previous studies showing that a microcavity featuring a good spectral alignment with the maximum emission of the emitting molecule enhances the emission intensity of the immobilised fluorophore (DeLouise and Ouyang, 2009;Palestino et al, 2009). When we partially masked the surface of the resazurin-modified MC-2 during 10 min exposure to LDH (Fig.…”

“…This is because the microcavity, which is a multilayer consisting of alternating porosities and a spacer layer in between, is able to enhance the emission of a fluorophore confined in the porous structure according to the Purcell effect (DeLouise and Ouyang, 2009;Koenderink, 2010;Poitras et al, 2003;Purcell, 1946;Qiao et al, 2010;Setzu et al, 2000). This particular pSi architecture has therefore been applied to fluorescence biosensor applications (Krismastuti et al, 2014;Palestino et al, 2008Palestino et al, , 2009Sciacca et al, 2009).…”

“…The application of porous silicon (pSi) as a sensitive platform for enzyme monitoring has been addressed in studies (DeLouise et al, 2005;Krismastuti et al, 2013;Martin et al, 2009;Palestino et al, 2009). The excellent performance of pSi-based sensors relies on the advantageous properties of pSi (Bosch et al, 2007;Jane et al, 2009;Janshoff et al, 1998;Lin et al, 1997;Sailor and Wu, 2009;Zhao et al, 2010).…”

Uso del silicio poroso funcionalizado con acetilcolinesterasa como plataforma de detección de arsénico (III)

“…Espectros de reflenctancia y picos del EOE de una monocopa de SiP fabricada con una corriente de 65 mA por 3 min, tomados a cada paso del proceso de funcionalización con AChE la solución de incubación para mostrar un ΔEOE. Es importante resaltar que un menor tamaño de poro admite menos infiltración de material orgánico (Palestino et al, 2009), por lo que para generar un desplazamiento de EOE se requiere una mayor concentración de la enzima en la solución de impregnación. En la monocapa de mayor diámetro de poro la concentración de 0.2 mg/ ml de AChE en la solución de impregnación generó mayor DEOE, por lo tanto, un mayor enlazamiento de la proteína sobre las paredes del poro, por lo que estas condiciones de preparación se eligieron para los estudios subsecuentes.…”

Biomolecule detection in porous silicon based microcavitiesviaeuropium luminescence enhancement

“…single layer and Bragg reectors), inltrated with emitting molecules. 18,22 The emitting molecules incorporated were glucose oxidase (GOX showing natural green uorescence) and uorescein isothiocyanate (FITC) labeled streptavidin. pSi microcavities demonstrated excellent uorescence enhancement for both proteins.…”