Detection and identification of grape varieties in must and wine using nuclear and chloroplast microsatellite markers

Baleiras-Couto, Margarida; Eiras-Dias, José Eduardo

doi:10.1016/j.aca.2005.09.076

Cited by 58 publications

(63 citation statements)

References 33 publications

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“…Due to the isobaric overlap of 87 Sr and 87 Rb, an effective Rb/Sr separation is a pre-requisite for the accurate determination of Sr isotope ratios by Q-ICP-MS. In order to perform this separation, ion-exchange chromatography was carried out using EDTA as eluent [6].…”

Section: Sr/ 86 Sr Ratio Analytical Determinationmentioning

confidence: 99%

“…The main constrain BIO Web of Conferences 02021-p.2 linked to their wide application in wines is associated to the quality and quantity of DNA extracted from the wine samples, given the fact that DNA can be highly degraded during the winemaking process (decanting and filtration), stabilization (clarification and racking) and aging [5]. Several DNA extraction protocols have been developed through the years [5][6][7]. The protocol developed by Pereira et al [7] has demonstrated to be able to extract DNA from small volume wine samples, 10 mL, and free of enzymatic inhibitors, therefore suitable for PCR-based technologies.…”

Section: Introductionmentioning

confidence: 99%

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Wine fingerprinting using a bio-geochemical approach

Fernandes

Pereira

Jorge

et al. 2015

BIO Web of Conferences

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Abstract.The wine sector is a billion euro business and therefore subjected to multiple attempts of fraudulent practices. This requires the development of rapid and reliable methods to detect such situations. Several methodologies have been developed based on the chemical profiles of the wines, but they are limited due to the environmental conditions that cannot be controlled. The use of DNA-based detection systems are an emergent research field that have been extended to a wide variety of food products and are still the most reliable methods for varietal identification. However these methods are not suitable for geographical determination. Soil related fingerprints have a primary role considering that there is a relationship between the elemental composition of wine and the composition of the provenance soil. WineBioCode is a project aiming to define the best strategy for wine authenticity based on a multidisciplinary approach. Two DNA-based strategies have been developed based on Real-time PCR and a label free optical biosensor platform. Both platforms enabled successful identification of specific DNA-targets when applied to Vitis vinifera L., and can be applied throughout the grape-wine chain. The methods are complementary and can be used in different situations, according to the requirements. The geographical evaluation has been assessed by the strontium 87 Sr/ 86 Sr isotope ratio determination involving soil evaluation in the vineyards followed by its assay in the wine samples. The results are being integrated in order to establish the best procedure to be undertaken for wine fingerprinting, including varietal composition and geographical origin, therefore fulfilling the requirements of the geographical denominations in wine certification.

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Section: Sr/ 86 Sr Ratio Analytical Determinationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Wine fingerprinting using a bio-geochemical approach

Fernandes

Pereira

Jorge

et al. 2015

BIO Web of Conferences

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“…Simple sequence repeats (SSR), with the advantages of co-dominance, high allele diversity, high stability and simple operation, have been widely used in genetic diversity analysis of grape resources, germplasm identification, genetic map construction, gene location (1,4,5,6,10,13,16,25,27,28,32,38), and other fields. With reproducible results, high reliability, simple technology and low cost, sequence-related amplified polymorphism (SRAP) has been applied in citrus, peaches, grape rootstock (3,24,44) and many other fruit tree species since its first exploitation in 2001. to date, only few papers have been published on the DnA based molecular markers analysis of chinese wild grapevine (22).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Chinese Wild Grape Species by SSR and SRAP Markers

Liu

Fan

Jiang

et al. 2012

Biotechnology & Biotechnological Equipment

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“…In addition, microsatellites could be used for genetic uniformity detection in grapevine plants propagated in vitro [27] and plant origin certification. Moreover, wine quality control and falsification, respectively, can be analysed from DNA extracted from wine [28]. Other DNA markers such as AFLPs, M-AFLPs, SAMPLs, ISSRs, and SNPs [29] have also been suggested for studies of genetic variation accumulated over centuries of cultivation and selection in different environments.…”

Section: Discussionmentioning

confidence: 99%

Genotyping of Vitis vinifera L. within the Slovak national collection of genetic resources

Dokupilová

Migliaro²,

Mihálik

et al. 2014

Open Life Sciences

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Microsatellites were used as a very effective tool for genetic diversity analysis and characterization of 51 grapevine (Vitis vinifera L.) accessions from the national collection of genetic resources. Genetic diversity was relatively high, 8.91 alleles were detected per analysed microsatellite locus in average, and fifty-one accessions were distinguished into 45 groups. Distribution of recent Slovak cultivars across the dendrogram accented both their genetic diversity and the effectiveness of the national breeding program in maintaining genetic diversity and generating new genetic variants. Each cultivar was different from the others and twelve of them contained 77.6% of the total genetic diversity of the whole analysed set. Microsatellite patterns were also able to confirm parentage in selected Slovak cultivars. An unusual phenomenon of triallelism was also detected in one of the analysed accessions. The present study has initiated molecular characterization within the national grapevine genetic resource collection and their comparison with well-established international cultivars.

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Detection and identification of grape varieties in must and wine using nuclear and chloroplast microsatellite markers

Cited by 58 publications

References 33 publications

Wine fingerprinting using a bio-geochemical approach

Wine fingerprinting using a bio-geochemical approach

Genetic Diversity of Chinese Wild Grape Species by SSR and SRAP Markers

Genotyping of Vitis vinifera L. within the Slovak national collection of genetic resources

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