Genetic Diversity of Chinese Wild Grape Species by SSR and SRAP Markers

Liu, Chonghuai; Fan, Xiucai; Jiang, Jianfu; Guo, Da‐Long; Sun, Han; Zhang, Ying; Feng, Jiancan

doi:10.5504/bbeq.2011.0146

Cited by 19 publications

(20 citation statements)

References 26 publications

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“…sylvestris (Lacombe et al, 2003), distributed from Middle East to European regions (Grassi et al, 2003a), for the development of genes tolerant to biotic and abiotic stresses that it is exposed to in new environments (Liu et al, 2012). sativa has been domesticated from wild Vitis vinifera subsp.…”

Section: Introductionmentioning

confidence: 99%

“…sativa has been domesticated from wild Vitis vinifera subsp. Nowadays, molecular markers are commonly being used to study genetic relationships among Vitis accessions (Grassi et al, 2003b;Liu et al, 2012). Over the past few centuries, historical evidence combined with ampelographic data has frequently been used to characterize grape germplasm and to investigate the origin and relationships among wild and cultivated individuals (Labra et al, 2002;Barth et al, 2009;Ocete et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Genetic characterization of some wild grape populations (Vitis viniferasubsp.sylvestris) of Zagros mountains (Iran) to indentify a conservation strategy

Doulati-Baneh

Mohammadi

Labra

et al. 2014

Plant Genet. Resour.

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In this study, the genetic constitution of wild grape populations distributed in the Zagros Mountains in the north-west part of Iran was investigated to assess their risk of extinction and to identify the most suitable conservation strategy. A total of 65 wild grapevine samples from five different populations were analysed using 23 SSR loci. Consistent allelic richness was detected in all the analysed populations (from 4.01 to 5.35). The observed heterozygosity and expected heterozygosity were high with mean values of 0.67 and 0.69, respectively. A modest heterozygosity deficiency was detected only in the Piranshahr and Sardasht-Shalmash populations. The neighbour-joining dendrogram clustered the accessions into three groups corresponding to the geographical distribution of the populations. Molecular data combined with field evaluation revealed that an ex situ conservation approach is the most suitable strategy for preserving the genetic richness of Iranian wild grape populations with consistent rare alleles (about 20%). We propose in situ preservation only in the case of the Sardasht-Ghasmarash population to ensure that evolutionary dynamic forces continue to influence plant adaptation and survival in response to environmental alterations.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic characterization of some wild grape populations (Vitis viniferasubsp.sylvestris) of Zagros mountains (Iran) to indentify a conservation strategy

Doulati-Baneh

Mohammadi

Labra

et al. 2014

Plant Genet. Resour.

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“…SSR and SRAP have also been used in genetic diversity analyses of the Chinese wild grape resources. Liu et al (2012a) reported on the relationship of 15 Chinese wild grape species based on the 10 microsatellite markers and 12 SRAP combinations. Fan et al (2015) assessed genetic polymorphisms of 126 individuals from five different geographical populations of wild Vitis ficifolia Bge.…”

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confidence: 99%

Genetic Diversity of Vitis davidii Accessions Revealed Using Microsatellite and Sequence-related Amplified Polymorphism Markers

Fan

Sun

Zhang

et al. 2018

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In this study, simple sequence repeat (SSR) and sequence-related amplified polymorphism (SRAP) markers were used to analyze the genetic diversity of 48 wild Vitis davidii accessions. A total of 78 distinct alleles were amplified by 11 SSR primers, and the average allele number was 8.8. The average observed heterozygosity (Ho) and expected heterozygosity (He) values were 0.785 and 0.814, respectively. The effective allele numbers ranged from 3.92 to 9.61. The average polymorphism information content (PIC) was 0.798. Twelve of 169 SRAP primer combinations were selected for SRAP analysis. A total of 188 bands were produced, and the average was 15.7 bands per primer combination; the average percentage of polymorphic bands was 84.0%. The average PIC was 0.76. The results of the clustering analysis based on SSR markers showed that the 48 wild V. davidii accessions could be classified into five main clusters and had a genetic similarity coefficient level of 0.68. The dendrogram obtained from the SRAP data showed that 48 wild V. davidii accessions could be classified into five main clusters and had a genetic similarity coefficient of 0.72. SSR and SRAP markers differentiated all accessions studied including those with a similar pedigree. We speculated on the origin of Ciputao 0941♀, Ciputao 0940♂, and Fu’an-ci-01 using SSR markers and used both SSR and SRAP markers to resolve homonymy. The result will be valuable for further management and protection of V. davidii germplasm resources.

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“…[15] SSR markers, however, are highly reproducible, [16] polymorphic in nature and locus specific. [17,18] Field evaluation of plant material Morphological data was collected for 28 genotypes of sugarcane. Data were collected for yield and tillers per plant during the year 2010.…”

Section: Ssr Analysismentioning

confidence: 99%

Association mapping of cane weight and tillers per plant in sugarcane

Bilal

Saeed

Nasir

et al. 2015

Biotechnology & Biotechnological Equipment

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Identification of agronomic traits tightly linked to molecular markers is a major footstep to implement the marker assisted selection in breeding programmes. Sugarcane is the major cash crop and the source of more than 2/3 of world sugar production. In this study, 20 simple sequence repeat (SSR) markers were used to find the linkage for sugarcane yield components; cane weight and tillers per plant using 28 sugarcane genotypes. In total, 114 alleles were found, ranging from 2-13 alleles per locus with a mean value of 5.7. High levels of polymorphism were detected through SSR amplifications among all genotypes studied. SSR markers, CEMB-14A and CEMB-14B, were found linked (P < 0.01) to tillers per plant while CEMB-5A, CEMB-5C and CEMB-7B were found associated (P < 0.05) with cane weight. Identification of new and novel SSR markers in sugarcane have potential use in genetic diversity studies, DNA fingerprinting, DNA markers-based screening of germplasm for breeding programme and also in determination of true hybrids in the offspring.

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Genetic Diversity of Chinese Wild Grape Species by SSR and SRAP Markers

Cited by 19 publications

References 26 publications

Genetic characterization of some wild grape populations (Vitis viniferasubsp.sylvestris) of Zagros mountains (Iran) to indentify a conservation strategy

Genetic characterization of some wild grape populations (Vitis viniferasubsp.sylvestris) of Zagros mountains (Iran) to indentify a conservation strategy

Genetic Diversity of Vitis davidii Accessions Revealed Using Microsatellite and Sequence-related Amplified Polymorphism Markers

Association mapping of cane weight and tillers per plant in sugarcane

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