Detection and characterization of human astrovirus and sapovirus in outpatients with acute gastroenteritis in Guangzhou, China

Luo, Xin; Deng, Jiankai; Mu, Xiaoping; Yu, Nan; Che, Xin

doi:10.1186/s12876-021-02044-5

Cited by 11 publications

(5 citation statements)

References 38 publications

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“…The findings from this study indicate that HuSaV was the second most prevalent cause of AGE outbreaks, following NoV, in the Chaoyang District of Beijing, China, between 2015 and 2021, which is consistent with observations in some other countries ( 6 - 7 ). Nonetheless, the genetic diversity of HuSaV found in this study differs from previous reports in both China ( 8 - 9 ) and other countries ( 10 - 13 ), where the GI genogroup was predominant.…”

Section: Discussioncontrasting

confidence: 99%

“…In contrast to other reports on the seasonal distribution of HuSaVs, outbreaks in Beijing typically take place during two periods, as depicted in Figure 1 . This variation significantly differs from the early spring occurrences reported in southern China ( 9 ), which may result from the considerable difference in latitudes between the two regions. In Japan, researchers have also discovered that the detection rate of HuSaV within a month varied across different years ( 6 ).…”

Section: Discussioncontrasting

confidence: 86%

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Genotypes Diversity of Acute Gastroenteritis Outbreaks Caused by Human Sapovirus — Beijing Municipality, China, 2015–2021

Yang¹,

Han²,

Qi³

et al. 2023

China CDC Weekly

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Introduction Human sapovirus (HuSaV) is an enteric virus responsible for sporadic cases and outbreaks of acute gastroenteritis (AGE) globally. A seven-year active surveillance study was conducted to investigate the molecular epidemiology of HuSaVs associated with AGE outbreaks in Chaoyang District of Beijing Municipality, China from January 2015 to December 2021. Methods Fecal and anal swab samples were obtained from patients experiencing AGE outbreaks. HuSaVs were identified through reverse transcription polymerase chain reaction (RT-PCR), and partial viral protein 1 (VP1) sequences (approximately 434 base pairs) were utilized for genotyping, single nucleotide polymorphism (SNP) analysis, and phylogenetic examination. Results HuSaVs were identified in 71 AGE outbreaks, demonstrating a detection rate of 10.5%, second only to norovirus. The primary demographic affected by HuSaV were children under the age of 5 in kindergarten settings. Infection rates tended to peak during two distinct periods: May to June and September to December. Upon genotyping, seven distinct genotypes emerged. GII.3 was the most prevalent, accounting for 54.9% of cases, followed by GI.1 (12.7%), GI.2 (9.9%), GII.5 (7.0%), GI.5 (2.8%), GI.6 (1.4%), GII.1 (1.4%), and untyped cases (9.9%). A phylogenetic analysis of GII.3 identified three distinct groups, with 15 notable SNPs observed. Conclusions This study offers a comprehensive analysis of the persistent prevalence of HuSaV outbreaks in Chaoyang District, Beijing Municipality, China. Over time, the diversity of HuSaV subtypes has shifted, and it is now recognized as the second leading viral agent responsible for AGE outbreaks. This highlights the importance of ongoing surveillance in the future.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 86%

Genotypes Diversity of Acute Gastroenteritis Outbreaks Caused by Human Sapovirus — Beijing Municipality, China, 2015–2021

Yang¹,

Han²,

Qi³

et al. 2023

China CDC Weekly

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“…We conducted a 6-consecutive-year monitoring and epidemiology study of HAdV in association with acute gastroenteritis from January 2015 to December 2021 to assess HAdV prevalence and to examine the genetic characteristics of viruses obtained from hospitalized children with acute diarrhea. HAdV was detected in 72 samples from these patients, and the detection rate was 4.10%, which is similar to the rates reported previously in Shanghai [ 9 ], Guangzhou [ 17 ], and China as a whole [ 18 ].…”

Section: Discussionsupporting

confidence: 88%

“…Human enteric adenovirus F40/41 was a major cause of acute gastroenteritis in children in Brazil from 2018 to 2020 [ 11 ]. In China, among patients with acute gastroenteritis, HAdV-F41 has been reported to be the predominant type, followed by HAdV species B and C [ 17 ]. In the current study of HAdV in Yunnan province, 13 strains were sequenced, 10 of which were HAdV-F41 (76.92%) and three of which were HAdV-C2 (23.08%), HAdV-F40 and other types were not found, presumably because of the small number of sequences analyzed.…”

Section: Discussionmentioning

confidence: 99%

Detection and complete genome sequence analysis of human adenovirus in children with acute diarrhea in Yunnan, China, 2015–2021

Cao,

Yang,

et al. 2024

Arch Virol

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The aims of this study were to determine the distribution and prevalence of gastroenteritis caused by human adenovirus (HAdV) in children in Yunnan province, China, in 2015–2021 and to identify preventive measures that can be taken to reduce morbidity and mortality in children.HAdV is a significant agent of diarrhea in children, but limited data are available regarding the epidemiology and genetic diversity of HAdV in children with diarrhea in Yunnan province, China. A total of 1754 fecal samples were subjected to real-time RT-PCR to detect and quantify HAdV. Positive samples were further analyzed using next-generation sequencing (NGS), and epidemiological data were analyzed as well.1754 patients with diarrhea were enrolled, of which 1041 were male and 713 were female (M:F ratio: 1.46). Seventy-two stool samples out of 1754 (4.10%) were positive for HAdV. The detection rates of all age groups varied from 2.50–4.78%. The highest incidence of HAdV was observed in children under 2 years of age, especially in children 12–24 months-old. From 2015–2021, the annual detection rate ranged from 1.62–12.26%. HAdV was detected throughout the year, but with marked seasonality. Children were most likely to be positive for HAdV in June and November. We detected HAdV in 15.53% (16/103) of samples collected in June and in 8.19% (14/171) of those collected in November. The entire viral genome was successfully sequenced for 13 of the 72 HAdV-positive samples, and 76.92% (10/13) of these were classified as genotype F41 and 23.08% (3/13) were classified as genotype C2.ConclusionsIn Yunnan province, children of all ages are susceptible to HAdV infection, but there has been marked variation in the yearly prevalence. The highest rate of HAdV detection was in June, followed by November. Priority should be given to disease prevention over the development of targeted antiviral therapies, and effective vaccines for preventing HAdV diarrhea are needed. It is also important to establish a surveillance system to collect relevant clinical and epidemiological data quickly in order to assess the potential risk of HAdV infection in children and to identify epidemic strains for the development of effective vaccines.

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“…Luo et al. 9 reported that rotavirus remained the primary viral pathogen followed by norovirus among outpatient children with AGE in Guangzhou, China. However, comprehensive investigations encompassing a wide range of pathogens including both bacteria and viruses are rarely reported for AGE patients.…”

Section: Introductionmentioning

confidence: 99%

Detection and Characterization of Bacterial and Viral Acute Gastroenteritis among Outpatient Children under 5 Years Old in Guangzhou, China

Luo,

Deng,

Luo

et al. 2024

The American Journal of Tropical Medicine and Hygiene

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Acute gastroenteritis (AGE) in children can be attributed to a multitude of bacterial and viral pathogens. The objective of this study was to investigate the epidemiology of bacterial and viral AGE in children and to compare clinical characteristics between single and multiple enteric pathogen infections. A total of 456 stool samples were collected from outpatient children under 5 years old with AGE, which were subsequently analyzed for nine bacteria and three viruses using the Luminex xTAG® Gastrointestinal Pathogen Panel. The presence of at least one pathogen was detected in 260 cases (57.0%), with Salmonella being the predominant agent, followed by norovirus, Campylobacter, and rotavirus. A total of 69 cases (15.1%) exhibited positive results for two or more enteric pathogens. Although certain co-infections demonstrated significant differences in primary clinical features compared with mono-infections, no statistical variance was observed in terms of disease severity. In outpatient children from southern China, Salmonella emerged as the most prevalent causative agent of AGE, succeeded by norovirus and Campylobacter. This study underscores the burden posed by coinfections and highlights the clinical characteristics associated with AGE when accompanied by coinfections among children under 5 years old.

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Detection and characterization of human astrovirus and sapovirus in outpatients with acute gastroenteritis in Guangzhou, China

Cited by 11 publications

References 38 publications

Genotypes Diversity of Acute Gastroenteritis Outbreaks Caused by Human Sapovirus — Beijing Municipality, China, 2015–2021

Genotypes Diversity of Acute Gastroenteritis Outbreaks Caused by Human Sapovirus — Beijing Municipality, China, 2015–2021

Detection and complete genome sequence analysis of human adenovirus in children with acute diarrhea in Yunnan, China, 2015–2021

Detection and Characterization of Bacterial and Viral Acute Gastroenteritis among Outpatient Children under 5 Years Old in Guangzhou, China

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