Detecção de peptídeos importantes para o diagnóstico da cisticercose bovina no immunoblot

Girotto, Aline; Pinto, Paulo Sérgio de Arruda; Dias, J C; Chaves, Leandro Siqueira; Ferreira, Hanna Carolina Campos

doi:10.1590/s0103-84782009005000053

Cited by 5 publications

(13 citation statements)

References 13 publications

(7 reference statements)

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“…Girotto (2007) found the opposite result for positive sera, with sera from bovines naturally infected with eggs of T. saginata presenting a higher number of reactive peptides than sera from experimentally infected bovines. The absence of a peptide to react with all samples coincides with the results obtained by other researchers in studies of bovine (Girotto et al, 2009), porcine (Tsang et al, 1991) and human (Tsang et al, 1989;Vaz et al, 1997) cysticercosis. Furthermore, the observed diversity of antibodies produced against reactive peptides confirms the heterogeneity of the host's immune response against the parasite (Vaz et al, 1997).…”

Section: Discussionsupporting

confidence: 89%

“…The immunoblot is used very little in the diagnosis of bovine cysticercosis, and despite obtaining promising results, Girotto et al (2009) emphasized the importance of performing variations in the protocol developed, in addition to testing other antigens, such as the total of T. crassiceps larvae, in order to confirm and verify the performance of this test for diagnostic purposes.…”

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confidence: 99%

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Relevant peptides of Taenia crassiceps for the diagnosis of bovine cysticercosis by immunoblot

Silva

Pinto

Ducas

et al. 2015

Arq. Bras. Med. Vet. Zootec.

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Given the limited knowledge about the diagnosis of bovine cysticercosis by immunoblot, the aim of this study was to assess the applicability of this test, identifying key peptides with diagnostic value. Immunoblot assays were performed using total larval antigen of Taenia crassiceps and 60 sera of positive bovines for cysticercosis (30 naturally and 30 experimentally infected with T. saginata eggs), 30 sera of negative bovines for cysticercosis and 30 sera of bovines with other diseases (fascioliasis, hydatidosis and tuberculosis). The peptides of greater diagnostic importance, in descending order of accuracy (%), were as follows: 6-8kDa (90.8%), 129-143kDa (74.2%), 99-105kDa (71.7%) and 14-19kDa (71.1%). Cross-reactions, due to fascioliasis and hydatidosis, were observed in the four intervals of peptides highlighted. The results demonstrate that the total antigen of T. crassiceps has peptides with a high diagnostic potential; therefore, the immunoblot is useful in the diagnosis of bovine cysticercosis.

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Section: Discussionsupporting

confidence: 89%

mentioning

confidence: 99%

Relevant peptides of Taenia crassiceps for the diagnosis of bovine cysticercosis by immunoblot

Silva

Pinto

Ducas

et al. 2015

Arq. Bras. Med. Vet. Zootec.

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“…Após a centrifugação, o sobrenadante foi coletado e a ele foi adicionado inibidor de protease (fluoreto de fenilmetilsulfonil -PMSF, Sigma Aldrich Co., St. Louis, MO, USA) na concentração de 2,5mM de extrato antigênico. O antígeno LVcra preparado foi mantido a -20°C (Girotto et al, 2009). …”

Section: Methodsunclassified

“…Para as análises pela técnica do Immunoblot, foi realizada a separação dos peptídeos do antígeno vesicular de T. crassiceps por eletroforese em gel de gradiente (4% a 12,5%) de poliacrilamida (SDS-PAGE) em sistema descontínuo, segundo Laemmli (1970), citado por Girotto et al (2009). Foi utilizado marcador de peso molecular de 6,500 a 200,000Da (Sigma S8445, Sigma Aldrich Co., St. Louis, MO, USA).…”

Section: Methodsunclassified

“…No entanto, a sensibilidade desse método é baixa, especialmente em infecções leves (Brasil, 1980;Minozzo et al, 2004). Dessa maneira, testes imunológicos, como o Enzyme Linked Immunosorbent Assay (ELISA) e o Immunoblot, que utilizam extratos antigênicos de larvas de T. saginata, T. solium e T. crassiceps, têm sido utilizados experimentalmente como métodos de diagnóstico ante mortem da cisticercose bovina, o que pode contribuir para aumentar a precisão dos dados oficiais em estabelecimentos inspecionados e servir de instrumento para a coleta de dados em estudos epidemiológicos e no monitoramento de parasitoses em animais de abate (Girotto et al, 2009;Santos, 2010).…”

Section: Introductionunclassified

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Diagnóstico do complexo teníase-cisticercose bovina em São João Evangelista, Minas Gerais, Brasil

Garro

Santos

Assis

et al. 2015

Arq. Bras. Med. Vet. Zootec.

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RESUMOCom o objetivo de diagnosticar a situação do complexo teníase-cisticercose bovina em Minas Gerais, Brasil, foi selecionado o município de São João Evangelista, onde foram coletadas amostras de sangue de 339 bovinos em 15 propriedades rurais, sorteadas aleatoriamente. Em cada propriedade, foi aplicado um questionário socioeconômico para a análise de fatores que favorecem a manutenção do complexo teníase-cisticercose bovina. Foi realizado também o diagnóstico de teníase humana por meio de exame coproparasitológico dos habitantes das propriedades. Encontrou-se a prevalência de 4,1% para cisticercose bovina e a frequência de 2,94% para teníase humana. Entre os fatores de risco para a manutenção do complexo teníase-cisticercose analisados, foi observada uma relação estatisticamente significativa (P=0,042) entre a ocorrência de cisticercose bovina e a ingestão de carne malpassada pelos entrevistados. Concluiu-se que a cisticercose bovina está presente no município de São João Evangelista, MG, em índices considerados endêmicos, sendo o consumo de carne malpassada e não inspecionada o principal fator de risco para a manutenção do complexo teníase-cisticercose, o que reforça a necessidade da adoção de medidas de controle com contínua vigilância epidemiológica e sanitária.Palavras-chave: bovino, cisticercose, ELISA, Immunoblot, teníase ABSTRACT In order to diagnose the situation of bovine taeniasis-cysticercosis complex in the state of Minas Gerais

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Development of the multi-epitope chimeric antigen rqTSA-25 from Taenia saginata for serological diagnosis of bovine cysticercosis

et al. 2018

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Bovine cysticercosis is a worldwide distributed zoonosis caused by the larval form of Taenia saginata present in bovine muscles. The diagnosis is based on the postmortem inspection at slaughterhouses and consists of the macroscopic visualization of lesions caused by cysticercosis in muscle sites. However, parasitized animals can pass unnoticed during sanitary inspection. Thus, the objective of this study was to characterize and evaluate the performance of different peptides from different regions of T. saginata for the cysticercosis diagnosis using enzyme-linked immunosorbent assay. We generated and evaluated a new recombinant protein chimera derived from the fusion of different peptides. We selected three distinct regions of T. saginata and predicted six peptides with antigenic potential (EP2–EP7). These peptides were analyzed individually and selected for generating a new chimeric recombinant protein. The new protein was termed rqTSA-25, and its performance rates were: 93.3% sensitivity (confidence interval (CI) = 76–98%), 95.3% specificity (CI = 82–99%), 93% positive predictive value (CI = 76–98%), 95% negative predictive value (CI = 82–99%), and 95% accuracy. In the immunoblot, this protein showed no false positive or false negative reaction. Thus, the use of rqTSA-25 is recommended for the diagnosis of bovine cysticercosis.

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Detecção de peptídeos importantes para o diagnóstico da cisticercose bovina no immunoblot

Abstract: I Detecção de peptídeos importantes para o diagnóstico da cisticercose bovina no immunoblot Detection of important peptides for bovine cysticercosis diagnosis in immunoblot RESUMO

Cited by 5 publications

References 13 publications

Relevant peptides of Taenia crassiceps for the diagnosis of bovine cysticercosis by immunoblot

Relevant peptides of Taenia crassiceps for the diagnosis of bovine cysticercosis by immunoblot

Diagnóstico do complexo teníase-cisticercose bovina em São João Evangelista, Minas Gerais, Brasil

Development of the multi-epitope chimeric antigen rqTSA-25 from Taenia saginata for serological diagnosis of bovine cysticercosis

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