Destruction of oral biofilms formedin situon machined titanium (Ti) surfaces by cold atmospheric plasma

Abstract

The decontamination of implant surfaces represents the basic procedure in the management of peri-implant diseases, but it is still a challenge. The study aimed to evaluate the degradation of oral biofilms grown in situ on machined titanium (Ti) discs by cold atmospheric plasma (CAP). ~200 Ti discs were exposed to the oral cavities of five healthy human volunteers for 72 h. The resulting biofilms were divided randomly between the following treatments: CAP (which varied in mean power, treatment duration, and/or the gas mixture), and untreated and treated controls (diode laser, air-abrasion, chlorhexidine). The viability, quantity, and morphology of the biofilms were determined by live/dead staining, inoculation onto blood agar, quantification of the total protein content, and scanning electron microscopy. Exposure to CAP significantly reduced the viability and quantity of biofilms compared with the positive control treatments. The efficacy of treatment with CAP correlated with the treatment duration and plasma power. No single method achieved complete biofilm removal; however, CAP may provide an effective support to established decontamination techniques for treatment of peri-implant diseases.

Cold Atmospheric Plasma: methods of production and application in dentistry and oncology

“…Idlibi et al treated Ti discs inoculated with biofilms with CAP [116]. The biofilms were divided randomly among the following treatments: CAP, diode laser, air-abrasion, and chlorhexidine.…”

Plasma in dentistry

“…Biofilms play a major role in the pathogenesis of various oral diseases, especially peri-implant mucositis. In recent studies, the use of NTAPP has been suggested for the removal of biofilms in general [120], and for the treatment of both peri-implant mucositis and peri-implantitis in particular [121–123]. …”

Plasma deposited silicon oxide films for controlled permeation of copper as antimicrobial agent

“…The basic procedure to prevent peri-implantitis or to support the healing process is the removal of microbial biofilms [3] and decontamination applying different methods. In recent studies the decontamination and conditioning of titanium implant surfaces by atmospheric plasma treatment has been suggested as an alternative method [4][5][6][7][8]. Atmospheric pressure jet-like discharges have been shown to be a promising tool for a variety of surface modifications since the gas temperature can be adjusted to room temperature or slightly above.…”