Designer nanoparticle: nanobiotechnology tool for cell biology

Raj, Deepak; Khan, Niamat

doi:10.1186/s40580-016-0082-x

Cited by 25 publications

(6 citation statements)

References 59 publications

(40 reference statements)

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“…The current review is focused on advances in the development of nanobiomaterials for applications in therapy, diagnosis, biosensing, bioanalysis and biocatalysis because nanobiomaterials for cell and organ chips [ 22 – 25 ], bioelectronic devices [ 26 , 27 ] and biological separation [ 28 ] have recently been reviewed in this journal.…”

Section: Application Of Engineered Biological Molecules To Nanobio/bimentioning

confidence: 99%

Biomolecular engineering for nanobio/bionanotechnology

Nagamune

2017

Nano Convergence

101

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Biomolecular engineering can be used to purposefully manipulate biomolecules, such as peptides, proteins, nucleic acids and lipids, within the framework of the relations among their structures, functions and properties, as well as their applicability to such areas as developing novel biomaterials, biosensing, bioimaging, and clinical diagnostics and therapeutics. Nanotechnology can also be used to design and tune the sizes, shapes, properties and functionality of nanomaterials. As such, there are considerable overlaps between nanotechnology and biomolecular engineering, in that both are concerned with the structure and behavior of materials on the nanometer scale or smaller. Therefore, in combination with nanotechnology, biomolecular engineering is expected to open up new fields of nanobio/bionanotechnology and to contribute to the development of novel nanobiomaterials, nanobiodevices and nanobiosystems. This review highlights recent studies using engineered biological molecules (e.g., oligonucleotides, peptides, proteins, enzymes, polysaccharides, lipids, biological cofactors and ligands) combined with functional nanomaterials in nanobio/bionanotechnology applications, including therapeutics, diagnostics, biosensing, bioanalysis and biocatalysts. Furthermore, this review focuses on five areas of recent advances in biomolecular engineering: (a) nucleic acid engineering, (b) gene engineering, (c) protein engineering, (d) chemical and enzymatic conjugation technologies, and (e) linker engineering. Precisely engineered nanobiomaterials, nanobiodevices and nanobiosystems are anticipated to emerge as next-generation platforms for bioelectronics, biosensors, biocatalysts, molecular imaging modalities, biological actuators, and biomedical applications.

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Section: Application Of Engineered Biological Molecules To Nanobio/bimentioning

confidence: 99%

Biomolecular engineering for nanobio/bionanotechnology

Nagamune

2017

Nano Convergence

101

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“…Magnetic nanoparticles are used for several medical applications such as drug delivery, hyperthermia, subcellular compartmental isolation [1–9]. In addition, nanotechnology has been considered as a potential technology that can contribute for the clinical studies including disease prevention, detection, diagnosis, imaging and treatment.…”

Section: Introductionmentioning

confidence: 99%

Development and characterisation of glucosamine sulphate magnetic nanoparticles for rheumatoid arthritis chemotherapy

Latha¹,

Selvamani²,

Raj³

2020

Micro & Nano Letters

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“…Such an approach will facilitate the analysis of the composition and dynamics of subcellular compartments upon drug exposure. Major limiting factor in determining the biomolecular composition of these dynamic organelles are the difficulties in isolating these organelles with high purity and high yield [ 17 ]. There are several existing methodologies such as density gradient centrifugation [ 18 ], antibody based immuno-precipitation [ 19 ], cationic silica based fractionation [ 20 , 21 ] and streptavidin based magnetic fractionation [ 22 , 23 ] that are used to isolate organelles such as plasma membrane with varied purity and yield.…”

Section: Introductionmentioning

confidence: 99%

Surface functionalization dependent subcellular localization of Superparamagnetic nanoparticle in plasma membrane and endosome

Raj

Khan²

2018

Nano Convergence

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In this article, we elaborate the application of thermal decomposition based synthesis of Fe3O4 superparamagnetic nanoparticle (SPMNP) in subcellular fractionation context. Here, we performed surface functionalization of SPMNP with phospholipids and dimercaptosuccinic acid. Surprisingly, we observed surface functionalization dependent SPMNP localization in subcellular compartments such as plasma membrane, endosomes and lysosomes. By using SPMNP based subcellular localization with pulse–chase methodology, we could use SPMNP for high pure-high yield organelle (plasma membrane, endosomes and lysosome) fractionation. Further, SPMNP that are distinctly localized in subcellular compartments can be used as technology for subcellular fractionation that can complement existing tools for cell biology research. As a future perspective, isolated magnetic organelles can be extended to protein/protein complex purification for biochemical and structural biology studies.

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Designer nanoparticle: nanobiotechnology tool for cell biology

Cited by 25 publications

References 59 publications

Biomolecular engineering for nanobio/bionanotechnology

Biomolecular engineering for nanobio/bionanotechnology

Development and characterisation of glucosamine sulphate magnetic nanoparticles for rheumatoid arthritis chemotherapy

Surface functionalization dependent subcellular localization of Superparamagnetic nanoparticle in plasma membrane and endosome

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