Surface functionalization dependent subcellular localization of Superparamagnetic nanoparticle in plasma membrane and endosome

Abstract

In this article, we elaborate the application of thermal decomposition based synthesis of Fe3O4 superparamagnetic nanoparticle (SPMNP) in subcellular fractionation context. Here, we performed surface functionalization of SPMNP with phospholipids and dimercaptosuccinic acid. Surprisingly, we observed surface functionalization dependent SPMNP localization in subcellular compartments such as plasma membrane, endosomes and lysosomes. By using SPMNP based subcellular localization with pulse–chase methodology, we could use SPMNP for high pure-high yield organelle (plasma membrane, endosomes and lysosome) fractionation. Further, SPMNP that are distinctly localized in subcellular compartments can be used as technology for subcellular fractionation that can complement existing tools for cell biology research. As a future perspective, isolated magnetic organelles can be extended to protein/protein complex purification for biochemical and structural biology studies.

Dynamic intracellular exchange of nanomaterials’ protein corona perturbs proteostasis and remodels cell metabolism

“…Most of the current biomolecular corona studies have focused on the blood plasma/serum, where immunoglobulins, apolipoproteins, complements, and coagulation cascade biomolecules are enriched on the nanoparticle surface and critically affect the nanoparticle-entailed pathophysiology and toxicity downstream ( 5 – 7 , 20 ). However, most targets of NMs for biomedical applications are at the subcellular level ( 21 – 25 ), and thus, understanding NMs involvement in intracellular events is important to ensure their therapeutic efficacy ( 4 ). To date, limited efforts have been devoted to delineating the uptake routes, and very few studies have focused on the molecular cell biology once the corona-coated NMs become resident in cells and/or tissues.…”

Chiral Magneto-Optical Properties of Supra-Assembled Fe3O4 Nanoparticles

“…The assembly of inorganic nanoparticles into well-defined suprastructures is an important and state-of-the-art approach for the fabrication of advanced functional materials. , The specific multiparticle interactions in different suprastructures, manifested as collective properties, often result in unique and interesting optical phenomena compared to those observed in their individual nanoparticle counterparts. A few examples are plasmonic hybridization modes such as dark mode and fano resonances in assembled plasmonic molecules, , quantum coupling in assembled colloidal quantum dots, , and collective chiral optical activity of the ensembles of chiral assembled inorganic nanostructures. , In addition, the supra-nanostructures assembled by metal and metal-oxide materials can exhibit chiral magneto-optical properties, and owing to their potential applications in advanced optoelectronic devices , and nanomedicine, , they have been attracting increased attention. However, the chiral magneto-optical responses related to these assembled structures have not yet been fully understood.…”

Surface modification of solid-state nanopore by plasma-polymerized chemical vapor deposition of poly(ethylene glycol) for stable device operation

“…One reasonable and representative approach was coating antifouling materials such as poly(ethylene glycol) (PEG) on the nanopore to prevent adsorption of biomolecules on the membrane surface [33,34,38]. PEG has been widely applied as an effective biorepelling agent, preventing nonspecific adherence of biomolecules on inorganic surfaces by passivating hydrophobic interaction sites on the surface or by steric repulsion of biomolecules [39][40][41][42]. The self-assembled polymeric layer successfully repelled single-stranded DNA (ssDNA) [33,34] or proteins [38] from adhering on silicon nitride (SiN x ) [33,38] or graphene surfaces [34], while a strong electric field in the nanopore region drove the biomolecules to move to the opposite chamber.…”