Design, preparation, and characterization of silk fibroin/carboxymethyl cellulose wound dressing for skin tissue regeneration applications

Abstract

Silk fibroin (SF) has been broadly applied in wound dressing fabrication because of its proper features for wound healing. In this work, we developed a carboxymethyl cellulose (CMC)/gelatin blend film with different concentrations of glycerol, and modified the optimized film with an SF layer through electrospinning process. Tensile strength and cell viability evaluation of blend films demonstrated that the glycerol content of 3% could be suitable as the substrate layer for the two‐layer wound dressing. The morphology of the blend film and electrospun nanofibers was obtained from scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). It concluded that structural changes had been occurred in both layers after cross‐linking with glutaraldehyde vapor. Further, it was shown that the mechanical properties of the two‐layer enhanced with the addition of SF. Moreover, the swelling ratio was higher than those of SF itself, due to the hydrophilic property of CMC/gelatin blend film. The biocompatibility of fibroblasts was investigated by MTT assay, and the coating showed an improvement in cell proliferation because of the cytocompatibility nature of SF. All results suggest that the prepared wound dressing could be a desirable candidate for wound healing applications.

Design of moxifloxacin encapsulated network hydrogel wound dressings: Evaluation of polymer‐drug, polymer‐blood, and polymer‐bio membrane interactions

“…[ 57 ] The incorporation of silk fibroin has improved the mechanical strength properties of the CMC/gelatin blend film and these films indicated the interection of the structure with the skin for better wound care applications. [ 58 ] The literature reports [ 59–64 ] and present research work indicated that the present material (AIG‐cl‐carbopol‐co‐poly(AAm) polymers) formed in the form of hydrogel films can act as potential wund dressing materials.…”

Nanomaterials supported by polymers for tissue engineering applications: A review

“…In addition, the rate of skin regeneration and angiogenesis in the animal model could be accelerated ( Ragothaman et al., 2021 ). In 2022, Farshi and coworkers have developed blends based on carboxymethyl cellulose and gelatin with different concentrations of glycerol and modified the film using silk fibroin layer using electrospinning technique ( Farshi et al., 2022 ). Various polymer based nanomaterials for skin tissue engineering applications are tabulated in Table 7 .…”

Emerging applications of nanotechnology in context to immunology: A comprehensive review

“…Apart from their significance in regenerative medicine, antimicrobials, drug delivery, and stem biology, Nanobiomaterials can be used for the treatment of diabetes mellitus 1 by generating supplemental oxygen required by islets until the formation of blood vessels ( Gholipourmalekabadi et al, 2016 ). Electrospinning uses the principles of nanobiotechnology for the preparation of biocompatible scaffolds which mimic collagen nanofibers providing protection and mechanical support at the injury site ( Farshi et al, 2022 ).…”