Design of functional guanidinium ionic liquid aqueous two-phase systems for the efficient purification of protein

Ding, Xueqin; Wang, Yuzhi; Zeng, Qun; Chen, Jing; Huang, Yanhua; Xu, Kaijia

doi:10.1016/j.aca.2014.01.030

Cited by 75 publications

(47 citation statements)

References 33 publications

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“…IL-based ABS have been used for the extraction of proteins and enzymes, while demonstrating to provide higher extraction efficiencies than the traditional PEG-based ABS [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34]. Alkylimidazolium-based ILs combined with phosphate-based salts were the most widely investigated phase-forming components of ABS [15].…”

Section: Introductionmentioning

confidence: 99%

Extraction and stability of bovine serum albumin (BSA) using cholinium-based Good's buffers ionic liquids

Taha

Quental

Correia

et al. 2015

Process Biochemistry

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Good's buffers ionic liquids (GB-ILs), composed of cholinium-based cations and Good's buffers anions, display self-buffering characteristics in the biological pH range, and their polarity and hydrophobicity can be easily tuned by a proper manipulation of their ions chemical structures. In this work, the extraction ability for bovine serum albumin (BSA) of aqueous biphasic systems (ABS) formed by polypropylene glycol 400 (PPG 400) and several GB-ILs was evaluated. ABS formed by PPG 400 and cholinium chloride ([Ch]Cl), GBs, and sucrose were also investigated for comparison purposes. It is shown that BSA preferentially migrates for the GB-IL-rich phase, with extraction efficiencies of 100%, achieved in a single-step. Dynamic light scattering, and circular dichroism (CD) and Fourier transform infrared (FTIR) spectroscopies were employed to evaluate the effect of the investigated cholinium-based GB-ILs on the BSA stability, and compared with results obtained for the respective GBs precursors, [Ch]Cl and sucrose, a well-known protein stabilizer. Molecular docking studies were also carried out to investigate on the binding sites of GB-IL ions to BSA. The experimental results confirm that BSA has a higher stability in GB-ILs than in any of the other compounds investigated.

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Section: Introductionmentioning

confidence: 99%

Extraction and stability of bovine serum albumin (BSA) using cholinium-based Good's buffers ionic liquids

Taha

Quental

Correia

et al. 2015

Process Biochemistry

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“…Given that, they have been used as functional molecules fixed with stationary phase or as additives in HPLC and CE for the separation of large bio-molecules [103][104][105]. In addition, TSILs served as the hydrophobic solvent in LLE and as the hydrophilic solvent in ATPS for the extraction of proteins were reported in the last ten years [106][107][108][109][110][111][112][113][114][115].…”

Section: Tsils For the Purification Of Proteins And Peptidesmentioning

confidence: 99%

“…Later, the same group prepared the magnetic chitosan graphene oxide composite coated with the functional F I G U R E 7 Schematic illustration of ATPS for purification of protein. Reprinted with permission from [112] tetraalkylguanidinium ILs as absorption material [113]. Unlike ATPS and traditional SPE for the extraction of proteins, SPE with magnetic material could avoid timeconsuming operation.…”

Section: Tsils For the Purification Of Proteins And Peptidesmentioning

confidence: 99%

Recent progress of task‐specific ionic liquids in chiral resolution and extraction of biological samples and metal ions

Cai

Zhao

et al. 2017

J of Separation Science

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Ionic liquids have been functionalized for modern applications. The functional ionic liquids are also called task-specific ionic liquids. Various task-specific ionic liquids with certain groups have been constructed and exploited widely in the field of separation. To take advantage of their properties in separation science, task-specific ionic liquids are generally used in techniques such as liquid-liquid extraction, solid-phase extraction, gas chromatography, high-performance liquid chromatography, and capillary electrophoresis. This review mainly covers original research papers published in the last five years, and we will focus on task-specific ionic liquids as the chiral selectors in chiral resolution and as extractant or sensor for biological samples and metal ion purification. K E Y W O R D Schiral ionic liquids, chiral resolution, metal extraction, protein purification, task-specific ionic liquids

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“…Liu et al [8] enhanced the selectivity and extraction efficiency of an SPME fiber for medium polar to polar compounds by using crown ether functionalized ILs. A series of cationic functional hexaalkylguanidinium ILs and anionic functional tetraalkylguanidinium ILs were synthesized and used to form IL aqueous two-phase systems for efficient purification of proteins [9]. ILs are efficient sorbent materials with excellent extraction and functionalization properties.…”

Section: Introductionmentioning

confidence: 99%

Development of a functionalized polymeric ionic liquid monolith for solid-phase microextraction of polar endocrine disrupting chemicals in aqueous samples coupled to high-performance liquid chromatography

Feng

Sun

et al. 2015

Anal Bioanal Chem

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Ionic liquids (ILs) have been efficiently used as a "designer sorbent" in sample preparation. A novel 1-(3-aminopropyl)-3-(4-vinylbenzyl)imidazolium 4-styrenesulfonate IL monomer was synthesized and copolymerized with 1,6-di(3-vinylimidazolium) hexane bishexafluorophosphate IL as cross-linking agent to prepare a cross-linked polymeric ionic liquids (PILs) monolith. Coupled to high-performance liquid chromatography (HPLC), the PILs monolith was used as a solid-phase microextraction (SPME) sorbent to extract some polar endocrine disrupting chemical (EDCs) such as estrogens, bisphenol A, and phthalate esters in aqueous samples. Preparation and extraction conditions were investigated and optimized to obtain satisfactory extraction efficiency. Limits of detection (LODs) of the proposed method for three steroid estrogens and bisphenol A were 0.25 and 0.2 μg L(-1), respectively, which were lower than or comparable to some other sample preparation methods. Intra- and inter-day repeatability for all the analytes was 2.2-12%. The monolith-to-monolith repeatability was 7.4-15%. The extraction performance of the method for analysis of target estrogens in treated domestic wastewater was investigated and compared with a dispersive liquid-liquid microextraction (DLLME) method. The proposed SPME method provided better sensitivity and higher resistance to matrix interferences.

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Design of functional guanidinium ionic liquid aqueous two-phase systems for the efficient purification of protein

Cited by 75 publications

References 33 publications

Extraction and stability of bovine serum albumin (BSA) using cholinium-based Good's buffers ionic liquids

Extraction and stability of bovine serum albumin (BSA) using cholinium-based Good's buffers ionic liquids

Recent progress of task‐specific ionic liquids in chiral resolution and extraction of biological samples and metal ions

Development of a functionalized polymeric ionic liquid monolith for solid-phase microextraction of polar endocrine disrupting chemicals in aqueous samples coupled to high-performance liquid chromatography

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