Design of a Tribocorrosion Bioreactor for the Analysis of Immune Cell Response to in Situ Generated Wear Products

Pourzal, Robin; Cichon, Robert; Mathew, Mathew T.; Pacione, Carol; Fischer, Alfons; Hallab, Nadim J.; Wimmer, Markus A.

doi:10.1615/jlongtermeffmedimplants.2014010305

Cited by 9 publications

(4 citation statements)

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“…As reported previously by Catelas et al (Catelas et al 2004) and Pourzal et al (Pourzal et al 2014), the wear particles generated from hip simulator using the serum as lubricant will have a characteristic particle size below 100 nm. Most of the particles generated in a protein-rich medium will have a tendency to form protein corona around the particles, which may stabilize the particles in solution.…”

Section: Discussionsupporting

confidence: 54%

Differential toxicity of processed and non-processed states of CoCrMo degradation products generated from a hip simulator on neural cells

et al. 2018

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Physico-chemical characteristics of the CoCrMo degradation products have played an important role in cytotoxicity and clinical complications on the orthopedic patients who have metal implants. Previous studies have limited reflection on the physicochemical characteristics of the degradation products generated in vivo, which are very different from individual metal particles and/or ions obtained from different commercial sources. In this study, we aimed to understand the differences in toxicity induced by the degradation products in as-synthesized form as well as those obtained after post-processing. The degradation products were generated using a hip-simulator by maintaining physiological conditions closer to in vivo and separated into two batches, one with processing by washing and drying called processed degradation products (PDP) and another batch as ‘as-synthesized’ degradation product (DP). We studied the dose-dependent toxicity response by neural cells derived from induced pluripotent stem cells. The results of the study show that as-synthesized DPs are more toxic to neural cells even at lower concentrations studied with evident low TC50 (1-5 μg/ml) concentrations compared to PDP (25 μg/ml). Flow cytometric analysis showed a significant (p<0.01) increase in uptake of the particles after 24h and corresponding ROS production in DP treated cells. RT-PCR analysis of oxidative specific gene expression showed, elevated mRNA levels of NADPH oxidase 1, nuclear transcription factor, Superoxide dismutase-2 and Glutaredoxin-2 in DP treated cells after 6h. The results of the study provided a clear evidence of the differential response of neural cells on the degradation products as a function of concentrations and their chemical nature.

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Section: Discussionsupporting

confidence: 54%

Differential toxicity of processed and non-processed states of CoCrMo degradation products generated from a hip simulator on neural cells

et al. 2018

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“…Also, it is important to note that all the electrochemical experiments were conducted under atmospheric conditions, not in CO 2 ‐enriched atmosphere. Therefore, a slight variation in pH can occur as reported in a previous study, but this pH change is inconsequential for the corrosion behavior of CoCrMo, and all tests were conducted under the same conditions.…”

Section: Discussionmentioning

confidence: 96%

In Vitro Evidence for Cell‐Accelerated Corrosion Within Modular Junctions of Total Hip Replacements

Bijukumar

Salunkhe

Morris

et al. 2019

Journal Orthopaedic Research

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Corrosion at modular junctions of total hip replacement (THR) remains a major concern today. Multiple types of damage modes have been identified at modular junctions, correlated with different corrosion characteristics that may eventually lead to implant failure. Recently, within the head-taper region of the CoCrMo retrieval implants, cell-like features and trails of etching patterns were observed that could potentially be linked to the involvement of cells of the periprosthetic region. However, there is no experimental evidence to corroborate this phenomenon. Therefore, we aimed to study the potential role of periprosthetic cell types on corrosion of CoCrMo alloy under different culture conditions, including the presence of CoCrMo wear debris. Cells were incubated with and without CoCrMo wear debris (obtained from a hip simulator) with an average particle size of 119 ± 138 nm. Electrochemical impedance spectroscopy (EIS) was used to evaluate the corrosion tendency, corrosion rate, and corrosion kinetics using the media after 24 h of cell culture as the electrolyte. Results of the study showed that there was lower corrosion resistance (p < 0.02) and higher capacitance (p < 0.05) within cell media from macrophages challenged with particles when compared with the other media conditions studied. The potentiodynamic results were also in agreement with the EIS values, showing significantly higher corrosion tendency (low E corr ) (p < 0.0001) and high I corr (p < 0.05) in media from challenged macrophages compared with media with H 2 O 2 solution. Overall, the study provides in vitro experimental evidence for the possible role of macrophages in altering the chemical environment within the crevice and thereby accelerating corrosion of CoCrMo alloy.

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“…Continuous passive motion on whole joints stimulated lubricin synthesis by chondrocytes 18 Macrophage cultures next to metal-on-metal prosthesis Tribocorrosion model Macrophages exposed to wear and corrosion products as they are generated 104 Cartilage-on-cartilage Shear Shear-induced damage affects chondrocyte viability and extracellular matrix integrity 16 Bovine osteochondral plug Free-swelling Osteochondral plugs are maintained viable for four weeks 19,20 12 PEROGLIO ET AL.…”

Section: Flexionmentioning

confidence: 99%

Relevance of bioreactors and whole tissue cultures for the translation of new therapies to humans

Peroglio

Gaspar

Zeugolis

et al. 2017

Journal Orthopaedic Research

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The purpose of this review is to provide a brief overview of bioreactor-based culture systems as alternatives to conventional two- and three-dimensional counterparts. The role, challenges, and future aspirations of bioreactors in the musculoskeletal field (e.g., cartilage, intervertebral disc, tendon, and bone) are discussed. Bioreactors, by recapitulating physiological processes, can be used effectively as part of the initial in vitro screening, reducing that way the number of animal required for preclinical assessment, complying with the 3R principles and, in most cases, allowing working with human tissues. The clinical significance of bioreactors is that, by providing more physiologically relevant conditions to customarily used two- and three-dimensional cultures, they hold the potential to provide a testing platform that is more predictable of a whole tissue response, thereby facilitating the screening of treatments before the initiation of clinical trials. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:10-21, 2018.

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Design of a Tribocorrosion Bioreactor for the Analysis of Immune Cell Response to in Situ Generated Wear Products

Cited by 9 publications

References 0 publications

Differential toxicity of processed and non-processed states of CoCrMo degradation products generated from a hip simulator on neural cells

Differential toxicity of processed and non-processed states of CoCrMo degradation products generated from a hip simulator on neural cells

In Vitro Evidence for Cell‐Accelerated Corrosion Within Modular Junctions of Total Hip Replacements

Relevance of bioreactors and whole tissue cultures for the translation of new therapies to humans

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