Design and fabrication of a microfluidic chip to detect tumor markers

Sun, Cuimin; You, Hui; Gao, Nailong; Chang, Junbiao; Gao, Qing; Xie, Yang; Xie, Yao; Xu, Ronald X.

doi:10.1039/d0ra06693a

Cited by 7 publications

(10 citation statements)

References 25 publications

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“…78 It combined the lateral displacement separation technique from Gao et al (Figure 3A) with an immobilized antibody to create an immunoassay embedded within the device. 63,78 The assay was performed within 10 min and demonstrated a serum extraction efficiency of 70% and 99.8% purity, similar to the parameters observed by Gao et al 63,78 Other devices also integrated plasma separation membranes and capillary action to filter undiluted whole blood and drive serum into detection zones without using external forces. 79,80 Biomarker detection was performed in-chip and validated with spiked samples.…”

Section: Microfluidic Devices For Capillary Samples (Plasma/ Serum)mentioning

confidence: 99%

Emerging Microfluidic Devices for Sample Preparation of Undiluted Whole Blood to Enable the Detection of Biomarkers

et al. 2023

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Blood testing allows for diagnosis and monitoring of numerous conditions and illnesses; it forms an essential pillar of the health industry that continues to grow in market value. Due to the complex physical and biological nature of blood, samples must be carefully collected and prepared to obtain accurate and reliable analysis results with minimal background signal. Examples of common sample preparation steps include dilutions, plasma separation, cell lysis, and nucleic acid extraction and isolation, which are time-consuming and can introduce risks of sample cross-contamination or pathogen exposure to laboratory staff. Moreover, the reagents and equipment needed can be costly and difficult to obtain in point-of-care or resource-limited settings. Microfluidic devices can perform sample preparation steps in a simpler, faster, and more affordable manner. Devices can be carried to areas that are difficult to access or that do not have the resources necessary. Although many microfluidic devices have been developed in the last 5 years, few were designed for the use of undiluted whole blood as a starting point, which eliminates the need for blood dilution and minimizes blood sample preparation. This review will first provide a short summary on blood properties and blood samples typically used for analysis, before delving into innovative advances in microfluidic devices over the last 5 years that address the hurdles of blood sample preparation. The devices will be categorized by application and the type of blood sample used. The final section focuses on devices for the detection of intracellular nucleic acids, because these require more extensive sample preparation steps, and the challenges involved in adapting this technology and potential improvements are discussed.

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Section: Microfluidic Devices For Capillary Samples (Plasma/ Serum)mentioning

confidence: 99%

Emerging Microfluidic Devices for Sample Preparation of Undiluted Whole Blood to Enable the Detection of Biomarkers

et al. 2023

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“…Also, for counting, detecting, and sorting of microparticles, the sheath flow focusing and sheathless focusing theory was applied in microfluidic devices [12]. Jian et al designed a microfluidic device that can successfully detect 6 out of 8 non-small cell lung cancer (NSCLC), and Hongmei et al explored with micro-ellipse filters for particles and there are small tunnels which gives less chance to escape [13][14][15][16][17][18][19][20][21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

“…Recent works on microfluidic devices are performed to detect the tumor markers including functions like blood plasma separation, microvalve operation, and antibody immobilization [16]. Those authors used 3D printing technology to fabricate the micromixers with different shapes of barriers like cylindrical, semi-cylindrical, conical, and semi-conical.…”

Section: Introductionmentioning

confidence: 99%

Design and Numerical Simulation of Biomimetic Structures to Capture Particles in a Microchannel

Yang

Joseph

Unnam

et al. 2022

Fluids

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The study of separating different sizes of particles through a microchannel has been an interest in recent years and the primary attention of this study is to isolate the particles to the specific outlets. The present work highly focuses on the design and numerical analysis of a microchip and the microparticles capture using special structures like corrugated dragonfly wing structure and cilia walls. The special biomimetic structured corrugated wing is taken from the cross-sectional area of the dragonfly wing and cilia structure is obtained from the epithelium terminal bronchioles to the larynx from the human body. Parametric studies were conducted on different sizes of microchip scaled and tested up in the range between 2–6 mm and the thickness was assigned as 80 µm in both dragonfly wing structure and cilia walls. The microflow channel is a low Reynolds number regime and with the help of the special structures, the flow inside the microchannel is pinched and a sinusoidal waveform pattern is observed. The pinched flow with sinusoidal waveform carries the particles downstream and induces the particles trapped in desired outlets. Fluid particle interaction (FPI) with a time-dependent solver in COMSOL Multiphysics was used to carry out the numerical study. Two particle sizes of 5 µm and 20 µm were applied, the inlet velocity of 0.52 m/s with an inflow angle of 50° was used throughout the study and it suggested that: the microchannel length of 3 mm with corrugated dragonfly wing structure had the maximum particle capture rate of 20 µm at the mainstream outlet. 80% capture rate for the microchannel length of 3 mm with corrugated dragonfly wing structure and 98% capture rate for the microchannel length of 2 mm with cilia wall structure were observed. Numerical simulation results showed that the cilia walled microchip is superior to the corrugated wing structure as the mainstream outlet can conduct most of the 20 µm particles. At the same time, the secondary outlet can laterally capture most of the 5 µm particles. This biomimetic microchip design is expected to be implemented using the PDMS MEMS process in the future.

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“…Detection tumor markers in human bodily fluids have been providing significant value for screening, diagnosis, and prognosis of cancer . Microfluidic chip technology has the potential to be a new way and platform for the detection of tumor markers due to its unique advantages, such as low consumption, high throughput, and good integration capabilities. , At the same time, fully integrated chip laboratory technology is also an important foundation in the point-of-care testing (POCT) field, by which all analysis steps can be integrated into one device …”

mentioning

confidence: 99%

“…1 Microfluidic chip technology has the potential to be a new way and platform for the detection of tumor markers due to its unique advantages, such as low consumption, high throughput, and good integration capabilities. 2,3 At the same time, fully integrated chip laboratory technology is also an important foundation in the point-of-care testing (POCT) field, by which all analysis steps can be integrated into one device. 4 When tumor markers in the blood are detected, a large number of blood cells will greatly interfere with the detection and reduce sensitivity and specificity.…”

mentioning

confidence: 99%

Detection of VEGF₁₆₅ in Whole Blood by Differential Pulse Voltammetry Based on a Centrifugal Microfluidic Chip

Wang

et al. 2022

ACS Sens.

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For the rapid and sensitive detection of vascular endothelial growth factor 165 (VEGF165) in clinical blood samples, a microfluidic sensing chip that integrates a centrifugal separation pretreatment unit and a composite nanosensing film was proposed in this paper. An efficient sensing strategy and method was established. The blood sample was first separated and extracted by centrifugal force on the centrifugal microfluidic chip within 5 min after injection. The separated plasma can be automatically transferred through the designed microchannels to the detection area integrated electrodes for subsequent differential pulse voltammetric detection. The Au NPs/MCH/Apt2 sensing film was constructed on the surface of the Au working electrode. A sandwich sensing strategy based on “double aptamers” and “nanoprobe” for VEGF165 detection was established, by which the synthetic Apt1/PThi/Au NP nanoprobe was applied to capture VEGF165 in plasma and bind to the sensing film. By this method, the detection limit of VEGF165 in whole blood was 0.67 pg/mL and the linear range was between 1 pg and 10 ng, which met the needs of clinical VEGF165 detection. It was illustrated that the proposed methodology based on the centrifugal microfluidic chip had potential application prospects in the development of the point-of-care testing fields.

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Design and fabrication of a microfluidic chip to detect tumor markers

Abstract: A microfluidic chip for detecting tumor markers integrated functions including blood plasma separation, microvalve operation, and antibody immobilization.

Cited by 7 publications

References 25 publications

Emerging Microfluidic Devices for Sample Preparation of Undiluted Whole Blood to Enable the Detection of Biomarkers

Emerging Microfluidic Devices for Sample Preparation of Undiluted Whole Blood to Enable the Detection of Biomarkers

Design and Numerical Simulation of Biomimetic Structures to Capture Particles in a Microchannel

Detection of VEGF₁₆₅ in Whole Blood by Differential Pulse Voltammetry Based on a Centrifugal Microfluidic Chip

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Design and fabrication of a microfluidic chip to detect tumor markers

Abstract: A microfluidic chip for detecting tumor markers integrated functions including blood plasma separation, microvalve operation, and antibody immobilization.

Cited by 7 publications

References 25 publications

Emerging Microfluidic Devices for Sample Preparation of Undiluted Whole Blood to Enable the Detection of Biomarkers

Emerging Microfluidic Devices for Sample Preparation of Undiluted Whole Blood to Enable the Detection of Biomarkers

Design and Numerical Simulation of Biomimetic Structures to Capture Particles in a Microchannel

Detection of VEGF165 in Whole Blood by Differential Pulse Voltammetry Based on a Centrifugal Microfluidic Chip

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Detection of VEGF₁₆₅ in Whole Blood by Differential Pulse Voltammetry Based on a Centrifugal Microfluidic Chip