Detection of VEGF₁₆₅ in Whole Blood by Differential Pulse Voltammetry Based on a Centrifugal Microfluidic Chip

Abstract

For the rapid and sensitive detection of vascular endothelial growth factor 165 (VEGF165) in clinical blood samples, a microfluidic sensing chip that integrates a centrifugal separation pretreatment unit and a composite nanosensing film was proposed in this paper. An efficient sensing strategy and method was established. The blood sample was first separated and extracted by centrifugal force on the centrifugal microfluidic chip within 5 min after injection. The separated plasma can be automatically transferred through the designed microchannels to the detection area integrated electrodes for subsequent differential pulse voltammetric detection. The Au NPs/MCH/Apt2 sensing film was constructed on the surface of the Au working electrode. A sandwich sensing strategy based on “double aptamers” and “nanoprobe” for VEGF165 detection was established, by which the synthetic Apt1/PThi/Au NP nanoprobe was applied to capture VEGF165 in plasma and bind to the sensing film. By this method, the detection limit of VEGF165 in whole blood was 0.67 pg/mL and the linear range was between 1 pg and 10 ng, which met the needs of clinical VEGF165 detection. It was illustrated that the proposed methodology based on the centrifugal microfluidic chip had potential application prospects in the development of the point-of-care testing fields.

Latest advances and perspectives of liquid biopsy for cancer diagnostics driven by microfluidic on-chip assays

“…Based on these advantages, it has been utilized to fabricate a large portion of microfluidic chips or build composite chips by bonding with other materials, such as glass and PC. 34…”

“…This PDMS disc with a minimal 500 μm channel depth was fabricated by the molding method and covered by a glass at the bottom. 34 With regard to the processing of silicon materials, the photolithography and wet etching techniques are two classic approaches. Silicon materials can be used for processing flow driving and control structures such as micropumps and microvalves or applied as a positive mold in the hot pressing and molding fabrication of polymers.…”

Enrichment and detection of VEGF165 in blood samples on a microfluidic chip integrated with multifunctional units

“…Previously, centrifugal force was used to separate plasma on a microfluidic chip and VEGF 165 in the plasma was in situ detected by DPV by our group. 13 Yang et al 14 designed a PDMS-glass hybrid microchip with four straight rectangular channels. Functionalized magnetic beads (MBs) were used to recognize and separate analytes from complex sample matrices and concentrate analytes in the magnetic capture/ detection area of the chip.…”

An automatic whole blood analyzer for renal function analysis with a centrifugal microfluidic device

“…25 In 2022, He et al developed a centrifugal microfluidic chip which integrated plasma separation and electrochemical detection of vascular endothelial growth factor 165. 26…”

“…25 In 2022, He et al developed a centrifugal microfluidic chip which integrated plasma separation and electrochemical detection of vascular endothelial growth factor 165. 26 Currently, centrifugal microfluidics can be combined with clinical chemical detection, which includes measurements based on absorbance. 27,28 This combination can be used for the automatic detection of whole blood sample indices.…”