Design and development of a miniaturised total chemical analysis system for on-line lactate and glucose monitoring in biological samples

Dempsey, Eithne; Diamond, Dermot; Smyth, Malcolm R.; Urban, G.; Jobst, G.; Moser, I.; Verpoorte, Elisabeth; Manz, Andreas; Widmer, Hans; Rabenstein, Kai; Freaney, R.

doi:10.1016/s0003-2670(97)90075-1

Cited by 109 publications

(49 citation statements)

References 13 publications

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“…The used of dry film resists (DFR) were originally developed for printed circuit board (PCB) fabrication (Dempsey et al 1997). It is also available as etching resists and solder mask (Svasek et al 1996).…”

Section: Epoxy Laminatementioning

confidence: 99%

Fabrication of microfluidic device channel using a photopolymer for colloidal particle separation

Yunus

Green

2010

Microsyst Technol

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We have developed a method of fabricating microfluidic device channels for bio-nanoelectronics system by using high performance epoxy based dry photopolymer films or dry film resists (DFRs). The DFR used was with a trademark name Ordyl SY355 from Elga Europe. The developing and exposing processes as well as the time taken in making the channels are recorded. Finally from those recorded methods, the accurate procedures and time taken for DFR development and exposure have been found and ultimately been consistently used in fabricating our channels. These channels were patterned and sandwiched in between two glass substrates. In our advance, the channel was formed for the colloidal particle separation system. They can be used for handling continuous fluid flow and particle repositioning maneuver using dielectrophoresis that have showed successful results in the separation.

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Section: Epoxy Laminatementioning

confidence: 99%

Fabrication of microfluidic device channel using a photopolymer for colloidal particle separation

Yunus

Green

2010

Microsyst Technol

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“…Nowadays, a wide array of bead particles (predominantly with a core of Sephadex type copolymers) with various surface characteristics are commercially available, and in the following section will be described procedures for real-time monitoring of cellular activities via immobilized live cells (15,72), isolation and on-column fluorimetric detection of DNA (15), optimization of immobilization protocols for proteins (74), as well as investigation of biomolecular association and dissociation processes as exploited in enzyme-linked immunosorbent assays (75) and affinity chromatographic methods (59,60,76). Yet before that, it might be of interest to compare the LOV with the socalled micro-total analysis systems (μTAS) (77), or as they lately have been termed Lab-on-a-Chips (LOC), which are exploited for bionanotechnological applications (78,79).…”

Section: X6 Microfluidic Devices: Lab-on-valvementioning

confidence: 99%

Flow Injection Analysis in Industrial Biotechnology

Hansen

Miró

2009

Encyclopedia of Industrial Biotechnology

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Flow injection analysis (FIA) is an analytical chemical continuous flow (CF) method which, in contrast to traditional CF procedures, does not rely on complete physical mixing (homogenization) of the sample and the reagent(s) or on attaining chemical equilibria of the chemical reactions involved. Exploiting controllable dispersion of the injected sample within the reagent‐containing carrier stream and strictly reproducible timing of all events taking place, it is based on measuring transient signals, which not only implies very high sampling rates but also, and most importantly, permits implementation of a number of novel methodologies that are not feasible when performed under batch conditions or by conventional CF procedures. Demonstrated for selected bioanalytical and technological applications encompassing cellular and enzymatic assays as well as monitoring of culture media, the principles and operational characteristics of FIA are first outlined, and then its downscaled/miniaturized sequels, that is, sequential injection analysis (SIA) and lab‐on‐valve (LOV), are detailed. Thus, in SIA the sample and reagents are, via the use of a multi‐position valve and an attached syringe pump operated under full programmable control, aspirated sequentially and then propelled forward allowing the sample and reagent(s) to be intermixed and, if called for, subjected to appropriate treatments before analyte detection. This infers that, merely minute sample/reagent volumes are consumed, hence leading to generation of small amounts of waste. In LOV this downscaling is taken further by using a small monolithic structure within which all sample manipulations and ultimate analyte detection under programmable control can be effected. Even bead materials with different surface groups/characteristics, including live cells, can be handled and utilized as demonstrated. Because the syringe pump in SIA and LOV can be used for accurately aspirating, propelling or even stopping the flow, these modi operandi allow fully to exploit the interplay between the kinetics and the thermodynamics of the chemical reactions involved, so that there are no restrictions whatsoever as to the chemistries that can be implemented, even if they entail multistep reactions. Representative bioanalytical examples of this interplay are presented.

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“…The biosensor chip with four individually addressable Pt electrodes with immobilized microbial strains was used in a flow system to measure oxygen consumption in the presence of assimilable microorganisms [126]. A miniaturized flow analytical system with an array of glucose and lactate biosensors was developed for on-line lactate and glucose monitoring in biological samples [127]. The complete system incorporates a microdialysis probe for intravascular monitoring and a silicon micromachined stack with an incorporated miniaturized flow cell/ sensor array.…”

Section: Miniaturization In Flow Electroanalysismentioning

confidence: 99%

Electroanalytical Flow Measurements–Recent Advances

2003

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As general trends in current development of chemical analysis, including electroanalysis, one can indicate a search for methods fast and multianalyte, for miniaturized measuring devices, mechanization and automation of analytical processes. In all these trends a significant role is played by measurements in flow conditions. The major advantage of flow electroanalysis is a possibility of utilizing a kinetic discrimination in potentiometric measurements and enhancement of mass transport in voltammetric techniques. Flow injection techniques provide shortening of time of a single analytical determination due to reproducible use of transient signal from the detector without need of obtaining a steady-state equilibrium signal. Electrochemical detection in HPLC gives often improved selectivity and detection limit for electroactive solutes, whereas in capillary electrophoresis it allows a convenient design of portable, integrated chips for field application. This review presents state-of-the -art of flow electroanalysis based on 226 cited literature references

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Design and development of a miniaturised total chemical analysis system for on-line lactate and glucose monitoring in biological samples

Cited by 109 publications

References 13 publications

Fabrication of microfluidic device channel using a photopolymer for colloidal particle separation

Fabrication of microfluidic device channel using a photopolymer for colloidal particle separation

Flow Injection Analysis in Industrial Biotechnology

Electroanalytical Flow Measurements–Recent Advances

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