Desenvolvimento de um método de análise de vitamina C em alimentos por cromatografa líquida de alta eficiência e exclusão iônica

Rosa, J. Santa; Godóy, Ronoel Luiz de Oliveira; Neto, J. Oiano; Campos, Rodrigo da Silveira; Freire, Cyntia Abreu; Silva, Aline Soares da; Souza, Rafael Santos de

doi:10.1590/s0101-20612007000400025

Cited by 51 publications

(51 citation statements)

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“…O ácido L-dehidroascórbico (DHA) também apresenta atividade vitamínica, pois é facilmente reduzido no organismo, e apresenta cerca de 80% da atividade vitamínica do AA. A atividade antioxidante da vitamina C envolve a doação de um elétron e a formação do radical livre ascorbato (ROSA et al, 2007).…”

Section: áCido Ascórbicounclassified

Fitoquímicos em amora-preta (Rubus spp)

Jacques

Zambiazi

2011

Sem. Ci. Agr.

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ResumoDentre as opções de espécies frutíferas com perspectivas de comercialização, a amoreira-preta (Rubus spp) se destaca como uma das mais promissoras. Esta é uma das espécies que tem apresentado um crescimento de área cultivada nos últimos anos no Rio Grande do Sul (principal produtor brasileiro). O consumo regular de frutas e hortaliças está associado com o baixo risco de incidência e mortalidade por câncer e doenças cardíacas, devido à presença de compostos oriundos do metabolismo secundário, especialmente flavonóides e antocianinas, os quais apresentam grande capacidade de reagir com radicais livres que causam estresse oxidativo, e portanto, contribuem na prevenção destas doenças. Na identificação dos compostos fenólicos na amora-preta foram encontrados os ácidos fenólicos e flavonóides. Dentre os flavonóides encontrados, destacam-se as antocianinas, que variam em sua concentração de acordo com o estágio de maturação das frutas. Tendo como base os valores encontrados na literatura sobre antocianinas e a grande variação entre os diferentes materiais genéticos, existe um grande potencial na produção de amora-preta visando a sua utilização como corante natural na indústria alimentícia e de medicamentos. Além dos compostos fenólicos, a amora-preta também apresenta outros fitoquímicos como às vitaminas C e E, além de carotenóides. Este trabalho tem como objetivo fazer uma revisão bibliográfica dos principais fitoquímicos presentes na amora-preta (Rubus spp). Palavras-chave: Amora-preta, fitoquímicos, antioxidantes AbstractAmong the options for fruit species with market prospects, the blackberry (Rubus spp) stands out as one of the most promising. This is a species that has shown an increase of cultivated area in recent years in Rio Grande do Sul (main Brazilian producer). Regular consumption of fruits and vegetables is associated with low risk of incidence and mortality from cancer and heart disease due to the presence of compounds derived from secondary metabolism, especially flavonoids and anthocyanins, which have great capacity to react with free radicals that cause oxidative stress, and therefore contribute to the prevention of these diseases. The phenolic acids and flavonoids were identificated in the group of phenolic compounds in blackberry. Among the flavonoids, stands out the anthocyanins, which vary in concentration according to the stage of maturation of fruits. Based on the antocyanin content related in literature and the great variation between different genetic materials, there is great potential in the production of blackberry and its utilization as a natural colorant in the food and pharmaceuticals industry. In addition to these compounds, the blackberry also has other phytochemicals such as vitamin C, vitamin E and carotenoids. This paper aims to review literature of the main phytochemicals in blackberry (Rubus spp).

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Section: áCido Ascórbicounclassified

Fitoquímicos em amora-preta (Rubus spp)

Jacques

Zambiazi

2011

Sem. Ci. Agr.

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“…Os humanos fazem parte do grupo de seres vivos que não são capazes de sintetizar vitamina C, a mesma possui importância para o organismo, atuando com função antiescorbútica, 13 síntese do colágeno, absorção do ferro e cura de resfriados. 2 Portanto, torna-se necessária uma ingestão adequada dessa vitamina, a maioria dos sucos frescos in natura é a melhor opção para ingerir a quantidade necessária de vitamina C diária.…”

Section: Discussionunclassified

Teor e estabilidade de vitamina C em sucos in natura e industrializados

et al. 2015

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ResumoBuscou-se quantificar e comparar o teor de vitamina C dos sucos frescos in natura e sucos industrializados prontos para beber, no momento do preparo, na abertura da embalagem e durante o armazenamento refrigerado. Assim como relacionar o teor encontrado com o custo para atingir a recomendação de ingestão diária desta vitamina, para adultos do sexo feminino e masculino. A amostragem foi composta de sucos de laranja, uva, manga, goiaba, tangerina e abacaxi, sendo utilizadas para os industrializados três marcas diferentes. As análises de vitamina C foram efetuadas no momento do preparo ou abertura da embalagem, no 4º, 8º e 12º dia, a partir do método de Tillmans. Para verificação dos custos, estes foram relacionados aos dados de: teor de vitamina C dos sucos, volume de um copo de 200 mL, recomendação de ingestão e preço gasto na aquisição. Teores superiores de vitamina C foram encontrados na maioria dos sucos frescos in natura, sendo os resultados dos industrializados superiores apenas nos sabores de tangerina (marca B, G) e manga (marca E). Em relação à estabilidade, não foi possível afirmar qual tipo de suco, natural ou industrializado, apresentou maior estabilidade. O maior custo-benefício foi obtido pelos sucos naturais, dependendo da época de safra da fruta. Desta forma, torna-se necessário ressaltar a importância do consumo de frutas frescas, tanto na forma de suco quanto na sua forma in natura, fator este, comprovado neste estudo.Palavras-chave: Ácido Ascórbico. Sucos. Frutas. AbstractIt was sought to compare the content of vitamin C between fresh in natura and industrialized juices, ready to drink, immediately after being prepared or in the opening package respectively, as well as, during the refrigerated storage. These results were also related to the daily recommended intake of the vitamin for female and male adults. The sampling was composed by orange, grape, mango, guava, tangerine and pineapple juices, three different industrialized brands were used one sample of each, but guava and tangerine were used just two brands of each. The vitamin C tests were performed, using Tillman's methodology, immediately after the preparation or packing opening and on the fourth, eighth and twelfth following days. To verify the costs, these data were related: juice vitamin C content, 200 ml cup volume, recommended intake and price paid on the purchase. Higher vitamin C content was found mostly on the fresh in natura juice samples, and the industrialized higher results were presented in only tangerine (brands B, G) and mango (brand E) flavors. Regarding the stability, it was not possible to define which kind of juice, fresh or industrialized showed de higher one. The most favorable cost benefit was presented on the fresh juices, depending on the fruits harvest. Thereby, is advisable the consumption of fresh fruits, as juices as well as on its in natura state, as it has been shown on the present study.

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“…The antioxidant activity of vitamin C involves the transfer of an electron to the free radical and the consequent formation of the radical ascorbate [109]. In addition, vitamin C acts synergistically with vitamin E, which regenerate the vitamin C has better antioxidant activity in hydrophilic media, and in aqueous phase of extracellular fluids, it is able to neutralize RO Si nt h ea q u e o u sp ha seb e fo r et h e yc a na t t a c k lipids.…”

Section: Nonenzymatic Antioxidant Systemsmentioning

confidence: 99%

Oxidative Stress: Noxious but Also Vital

Bagatini¹,

Jaques²,

Oliveira³

et al. 2018

Novel Prospects in Oxidative and Nitrosative Stress

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The imbalance between reactive oxygen species (ROS) production and antioxidant defenses determines the condition called oxidative stress. When there is an increase in ROS production or a decrease in the antioxidant defenses, this systemic antioxidant/pro-oxidant imbalance may lead to the accumulation of oxidative damage, which, in turn, may lead to a modification of biomolecules. These consist of reactions resulting in protein adducts, DNA oxidation, and formation of lipid peroxides, which, in turn, reduce the cellular functional capacity and increase the risk of disease development. The body has natural scavenging systems against free radicals and other reactive species. However, sometimes the endogenous antioxidant capacity is exceeded by the production of ROS. When this occurs, exogenous antioxidants exert important function for the human health. These bioactive compounds act preventing and neutralizing the formation of new reactive species and free radical s .I ns o m ec a s e s ,a n increase of ROS can help the host to resolve an infection or even to control the tumor growth. Finally, the levels of ROS can be perceived by signal transduction pathways involving known targets(i.e.,p53,Ras,andNF-κB) and regulate physiopathological events such as the cellular cycle, apoptosis, and inflammation.Keywords: reactive species, cellular oxidation, antioxidant system, health, disease © 2018 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Cellular respiration and generation of reactive species in the mitochondria: implications in cell viability and agingOxidative phosphorylation is the center of energy metabolism in plants, animals and several microbial life forms [1]. In eukaryotes, this process occurs in mitochondria. The mitochondria is a cytoplasmic organelle surrounded by two membranes, outer and inner membrane, which main function is the production of most of the phosphate compounds necessary for the energetic balance of the cell. In addition, other functions such as the regulation of the body'sheatgeneration [2][3][4] programmed cell death [5][6][7], reactive oxygen species (ROS) generation and cell signaling [8] is also associated with mitochondria. Cellular vitality is directly related to mitochondria, and mitochondrial dysfunctions are frequent causes of accidental cell death [5,[9][10][11], cancer [12, 13], diabetes [14][15][16] and neurodegenerative diseases [17][18][19], among others.The characterization of the respiratory electron chain could be performed in studies using the fractionation of its components by certain detergents that at low concentrations break the interactions between proteins and lipids in the membranes, leaving associations between proteins intact [20]. In electron transport chain, through this process, four protein complexes were found....

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Desenvolvimento de um método de análise de vitamina C em alimentos por cromatografa líquida de alta eficiência e exclusão iônica

Cited by 51 publications

References 18 publications

Fitoquímicos em amora-preta (Rubus spp)

Fitoquímicos em amora-preta (Rubus spp)

Teor e estabilidade de vitamina C em sucos in natura e industrializados

Oxidative Stress: Noxious but Also Vital

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