Descriptive analysis of the prevalence and the molecular epidemiology of Mycobacterium avium complex-infected pigs that were slaughtered on the main island of Okinawa

Hibiya, Kenji; Kazumi, Yuko; Nishiuchi, Yukiko; Sugawara, Isamu; Miyagi, Kazufumi; Oda, Y.; Oda, Eiji; Fujita, Jiro

doi:10.1016/j.cimid.2009.03.002

Cited by 14 publications

(9 citation statements)

References 55 publications

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“…Sample collection was conducted by veterinary wildlife specialists to avoid contamination of samples during transportation and at the laboratory (Pate et al 2016). The fecal and tissue samples were immediately processed using 2% sodium hydroxide (Hibiya et al 2010;Ito et al 2018b). The inocula obtained from both tissues and feces were inoculated onto a 2% Ogawa slant culture tube (Kyokuto Pharmacy, Tokyo, Japan) and into BBLe (Difco Laboratories, Sparks, Maryland, USA) mycobacterium growth indicator tube liquid media supplemented with polymyxin B, amphotericin B, nalidixic acid, trimethoprim, and azlocillin (Becton Dickinson, Franklin Lakes, New Jersey, USA) and incubated at 37 C for 4 wk.…”

Section: Isolation Of Bacteriamentioning

confidence: 99%

Isolation and Antimicrobial Susceptibilities of Nontuberculous Mycobacteria From Wildlife in Japan

Odoi

Ohya

Moribe

et al. 2020

Journal of Wildlife Diseases

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Nontuberculous mycobacteria (NTM) are opportunistic pathogens of humans and animals and are transmitted among the environment, wildlife, livestock, and humans. The aim of this study was to investigate the rate of isolation and antimicrobial susceptibility of NTM in wildlife. In total, 178 samples of feces ( n=131) and tissues ( n=47) were collected from 11 wildlife species in Gifu Prefecture and Mie Prefecture, Japan, between June 2016 and October 2018. We isolated NTM from 15.3% (20/131) of fecal samples using Ogawa medium, and isolates were identified by sequencing the rpoB and hsp65 genes. The rpoB sequences were compared with those from other strains of human and environmental origin. The NTM isolates were obtained from sika deer ( Cervus nippon), wild boar ( Sus scrofa), Japanese monkey ( Macaca fuscata), raccoon dog ( Nyctereutes procyonoides), masked palm civet ( Paguma larvata), and Japanese weasel ( Mustela itatsi) and were classified as rapidly growing mycobacteria (RGM) and slowly growing mycobacteria (SGM). The 12 RGM identified were Mycolicibacterium peregrinum ( n=5), Mycolicibacterium fortuitum ( n=3), Mycolicibacterium septicum ( n=3), and Mycolicibacterium thermoresistibile ( n=1), and the eight SGM were Mycobacterium paraense ( n=4), Mycolicibacter arupensis ( n=2), Mycolicibacter virginiensis ( n=1), and Mycobacterium nebraskense ( n=1). The NTM from wildlife showed ≥99% similarity with strains from different sources including humans. The RGM were susceptible to the antimicrobial agents tested except for M. fortuitum, which was resistant to azithromycin and clarithromycin. The SGM showed multiple drug resistance qualities but were susceptible to amikacin, clarithromycin, and rifabutin. These results indicate that wildlife may be reservoir hosts of NTM in Japan. The presence of antimicrobial-resistant NTM in wildlife suggests that the trends of NTM antimicrobial susceptibility in wildlife should be monitored.

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Section: Isolation Of Bacteriamentioning

confidence: 99%

Isolation and Antimicrobial Susceptibilities of Nontuberculous Mycobacteria From Wildlife in Japan

Odoi

Ohya

Moribe

et al. 2020

Journal of Wildlife Diseases

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“…As micobacterioses, diferentes da tuberculose, são associadas a micobactérias não tuberculosas ou atípicas, pertencentes ao Complexo Mycobacterium avium (MAC) (Cousins et al 2004). O MAC é composto por patógenos oportunistas (Hibiya et al 2010), presentes em elevado número de espécies animais e em humanos (Ingen et al 2010). Nestes, a infecção por essas bactérias atípicas ocorre principalmente em indivíduos imunodeprimidos; M. avium subsp.…”

Section: Introductionunclassified

“…Trabalhos mostram frequência elevada de isolamento de M. avium em tecidos de suínos abatidos (Hibiya et al 2010, Ingen et al 2010. No Brasil o MAC é considerado o mais importante grupo de bactérias envolvido em infecções desse tipo em suínos.…”

Section: Introductionunclassified

Caracterização histológica e imuno-histoquímica das lesões de tuberculose em bovinos e de linfadenite granulomatosa em suínos

et al. 2015

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RESUMO.-Mycobacterium sp. induz inflamação granulomatosa em diferentes espécies animais. Mycobacterium bovis e o complexo Mycobacterium avium são importantes pató-genos de bovinos e suínos e podem causar infecção em humanos, principalmente imunossuprimidos. Perdas na produção, barreiras comerciais e prejuízos por condenação de carcaças em abatedouro/frigorífico estão atrelados à ocorrência dessas infecções, com prejuízos econômicos signifi- Mycobacterium bovis and the Mycobacterium avium complex are important cattle and swine pathogens that can also infect humans, especially those immunosuppressed. Losses in production, commercial barriers and carcasses condemnations in slaughtering are related to this infection, which implies in large economic losses. It was carried out a study on bovine tuberculosis lesions and granulomatous lymphadenitis in pigs, diagnosed by the Setor de Patologia Veterinária from the Universidade Federal do Rio Grande do Sul (SPV-UFRGS), Brazil, between January 2007 and December 2011. Data concerning breed, sex, age and clinical history were retrieved from the files and analyzed. Histological features of the lesions in lymph nodes and lungs were evaluated in Hematoxylin-Eosin staining. Multinucleated giant cells were predominant in bovine tuberculosis lesions and epithelioid macrophages were abundant in swine. Ziehl-Neelsen and Masson's trichrome techniques were used respectively to demonstrate the alcohol-acid resistant bacillus and fibrous connective tissue in the lesions. The immunohistochemistry technique was performed to characterize the lymphocytic infiltrate. Anti-CD3 antibodies were utilized to immunolabeling lymphocytes T; and anti-CD79αcy to lymphocytes B. Lymphocytes T were predominant in both species lesions; confirmed statistically by paired t test, which showed significantly differ means of T and B lymphocytes, with t=5,501 (p<0.001) for the bovines tuberculosis lesions, and t=5.826 (p<0.001), for the cases of pigs lymphadenitis. In addition, macrophages were immunolabeled by antibody anti-CD68 to bovine, and lysozyme to swine; and polyclonal anti-Mycobacterium tuberculosis antibody were used successfully to detect Mycobacterium sp. in bovine and swine lesions, apart from in the swine samples there was also anti-Mycobacterium avium immunolabelling.INDEX TERMS: Mycobacterium spp., infectious diseases, cattle, swine, immunohistochemistry.

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“…In pigs, the pathogen has been found in the granulomatous lesion in the mesenteric lymph nodes, liver, spleen and jejunum [ 15 , 30 ]. Most Mah -infected pigs usually have no clinical signs, and lesions are often detected at meat inspection [ 6 ].…”

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confidence: 99%

“…Most MAC are associated with subclinical granulomas in the lymph nodes of pigs [ 6 ] and cattle [ 3 ] detected in slaughterhouses. Diarrhea can occur in immunocompromised children [ 7 ] and HIV infected patients [ 13 ].…”

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Systemic mycobacteriosis caused by ‘<i>Mycobacterium avium</i> subspecies <i>hominissuis</i>’ in a 14-month-old Japanese black beef steer

Komatsu

Inaba

Kondo

et al. 2017

The Journal of Veterinary Medical Science

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A 14-month-old Japanese black beef steer presented with severe chronic diarrhea and emaciation and was euthanized. Postmortem examination showed thickened and corrugated intestinal mucosa and enlarged granulomatous mesenteric lymph nodes with caseating necrosis. Numerous epithelioid cells and multinucleated giant cells infiltrated in the lamina propria and the submucosal tissue of the intestines. These cells were also observed in the systemic organs. Many acid-fast bacilli were detected in the cytoplasm of these cells and were identified as ‘Mycobacterium avium subsp. hominissuis’ (Mah) on the basis of the results of molecular examinations and immunohistochemistry. These findings indicate that Mah can cause systemic mycobacteriosis, and this unique infection needs to be distinguished from Johne’s disease and tuberculosis in cattle.

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Descriptive analysis of the prevalence and the molecular epidemiology of Mycobacterium avium complex-infected pigs that were slaughtered on the main island of Okinawa

Cited by 14 publications

References 55 publications

Isolation and Antimicrobial Susceptibilities of Nontuberculous Mycobacteria From Wildlife in Japan

Isolation and Antimicrobial Susceptibilities of Nontuberculous Mycobacteria From Wildlife in Japan

Caracterização histológica e imuno-histoquímica das lesões de tuberculose em bovinos e de linfadenite granulomatosa em suínos

Systemic mycobacteriosis caused by ‘<i>Mycobacterium avium</i> subspecies <i>hominissuis</i>’ in a 14-month-old Japanese black beef steer

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