RESUMO.-Mycobacterium sp. induz inflamação granulomatosa em diferentes espécies animais. Mycobacterium bovis e o complexo Mycobacterium avium são importantes pató-genos de bovinos e suínos e podem causar infecção em humanos, principalmente imunossuprimidos. Perdas na produção, barreiras comerciais e prejuízos por condenação de carcaças em abatedouro/frigorífico estão atrelados à ocorrência dessas infecções, com prejuízos econômicos signifi- Mycobacterium bovis and the Mycobacterium avium complex are important cattle and swine pathogens that can also infect humans, especially those immunosuppressed. Losses in production, commercial barriers and carcasses condemnations in slaughtering are related to this infection, which implies in large economic losses. It was carried out a study on bovine tuberculosis lesions and granulomatous lymphadenitis in pigs, diagnosed by the Setor de Patologia Veterinária from the Universidade Federal do Rio Grande do Sul (SPV-UFRGS), Brazil, between January 2007 and December 2011. Data concerning breed, sex, age and clinical history were retrieved from the files and analyzed. Histological features of the lesions in lymph nodes and lungs were evaluated in Hematoxylin-Eosin staining. Multinucleated giant cells were predominant in bovine tuberculosis lesions and epithelioid macrophages were abundant in swine. Ziehl-Neelsen and Masson's trichrome techniques were used respectively to demonstrate the alcohol-acid resistant bacillus and fibrous connective tissue in the lesions. The immunohistochemistry technique was performed to characterize the lymphocytic infiltrate. Anti-CD3 antibodies were utilized to immunolabeling lymphocytes T; and anti-CD79αcy to lymphocytes B. Lymphocytes T were predominant in both species lesions; confirmed statistically by paired t test, which showed significantly differ means of T and B lymphocytes, with t=5,501 (p<0.001) for the bovines tuberculosis lesions, and t=5.826 (p<0.001), for the cases of pigs lymphadenitis. In addition, macrophages were immunolabeled by antibody anti-CD68 to bovine, and lysozyme to swine; and polyclonal anti-Mycobacterium tuberculosis antibody were used successfully to detect Mycobacterium sp. in bovine and swine lesions, apart from in the swine samples there was also anti-Mycobacterium avium immunolabelling.INDEX TERMS: Mycobacterium spp., infectious diseases, cattle, swine, immunohistochemistry.
Pesq. Vet. Bras. 33(3):305-309, março 2013 305 RESUMO.-Actinobacilose é uma doença infecciosa, não contagiosa, geralmente crônica, caracterizada por reação inflamatória piogranulomatosa que ocorre em bovinos e, menos comumente, em ovinos, suínos e equinos. Tecidos moles de cabeça, pescoço e linfonodos regionais são afetados. Neste estudo foram compilados e avaliados 18 casos de bovinos dos livros de registro de janeiro de 1997 a maio de 2011 com diagnóstico de actinobacilose. As lesões foram caracterizadas histologicamente, avaliadas por técnicas de histoquímica e imuno-histoquímica. No exame histológico foram evidenciados piogranulomas típicos de actinobacilose contendo
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