Description of Niveispirillum fermenti gen. nov., sp. nov., isolated from a fermentor in Taiwan, transfer of Azospirillum irakense (1989) as Niveispirillum irakense comb. nov., and reclassification of Azospirillum amazonense (1983) as Nitrospirillum amazonense gen. nov

Abstract

A taxonomic study was carried out on a novel aerobic bacterial strain (designated CC-LY736(T)) isolated from a fermentor in Taiwan. Cells of strain CC-LY736(T) were Gram-stain negative, spiral-shaped and motile by means of a monopolar flagellum. Strain CC-LY736(T) shared the greatest degree of 16S rRNA gene sequence similarity to Azospirillum irakense DSM 11586(T) (97.2 %), Rhodocista centenaria JCM 21060(T) (96.3 %) and Rhodocista pekingensis JCM 11669(T) (96.1 %). The major fatty acids were C16:0, C16:1 ω5c, C19:0 cyclo ω8c, C18:1 ω7c/C18:1 ω6c, C16:0 3-OH and C18:1 2-OH. The predominant polar lipids included phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidyldimethylethanolamine and two unidentified glycolipids. The common major respiratory quinone was ubiquinone Q-10 and predominant polyamines were sym-homospermidine and putrescine. The DNA G+C content of strain CC-LY736(T) was 67.6 ± 0.1 mol %. During phylogenetic analysis, strain CC-LY736(T) formed a unique phyletic lineage associated with Rhodocista species. However, the combination of genetic, chemotaxonomic and physiological data clearly indicated that strain CC-LY736(T) was a novel representative of the family Rhodospirillaceae. Based on the polyphasic comparison, the name Niveispirillum fermenti gen. nov., sp. nov. is proposed; the type strain of the type species is CC-LY736(T) (= BCRC 80504(T) = LMG 27263(T)). In addition, the reclassifications of Azospirillum irakense as Niveispirillum irakense comb. nov. (type strain KBC1(T) = ATCC 51182(T) = BCRC 15764(T) = CIP 103311(T)), and Azospirillum amazonense as Nitrospirillum amazonense gen. nov., sp. nov. (type strain Am14(T) = ATCC 35119(T) = BCRC 14279(T) = DSM 3787(T)) are proposed based on the polyphasic taxonomic data obtained in this study.

Influência do uso de um mix de bactérias diaztróficas na biometria e no conteúdo de clorofila de plantas de cana-de-açúcar

“…Os fatores foram arranjados em esquema bifatorial (5x2), com o objetivo de testar cinco variedades de cana-de-açúcar e o uso de bactérias diazotróficas (com e sem uso de inoculante bacteriano), totalizando 10 tratamentos. As variedades de cana-de-açúcar utilizadas foram: RB867515; RB966928; RB016814; RB016818 e RB016819 e o inoculante bacteriano utilizado continha cinco estirpes de bactérias diazotróficas, sendo elas: BR11281T = PAL-5T de Gluconacetobacter diazotrophicus descrita por Cavalcante e Döbereiner (1988); BR11335 = HRC54 de Herbaspirillum seropedicae, descrita por Baldani et al (1986); BR11504 = HCC103 de Herbaspirillum rubrisubalbicans, descrita por Baldani et al (1996); BR11366T = PPe8T de Paraburkholderia tropica, anteriormente pertencente ao gênero Burkholderia, descrito por Reis et al (2004) e recentemente reclassificado por Oren e Garrity (2015); e BR11145 = CBAMc de Nitrospirillum amazonense, anteriormente pertencente ao gênero Azospirillum, descrito por Magalhães et al (1983) e reclassificado por Lin et al (2014). Essas bactérias foram previamente testadas e selecionadas por Oliveira et al (2002Oliveira et al ( , 2006 e foram fornecidas pela Coleção de Bactérias Diazotróficas da Embrapa Agrobiologia -CBR Johanna Döbereiner.…”

Reverse Biosynthesis: Generating Combinatorial Pools of Drug Leads from Enzyme‐Mediated Fragmentation of Natural Products

“…Niveispirillum irakense is able to catabolise 1 , an anabolic product of S. pilosus , in order to provide succinic acid as an alternative carbon source. N. irakense was discovered in the rhizosphere of rice crops in Iraq; the name of the species was recently transferred from the original Azospirillum irakense . Siderophore‐producing bacteria often cohabit in these environments and provide benefit to the community by restricting the growth of pathogens through iron deprivation.…”

Azospirillum agricola sp. nov., a nitrogen-fixing species isolated from cultivated soil

“…T (Lavrinenko et al, 2010), A. rugosum DSM 19657 T (Young et al, 2008) and Azospirillum zeae LMG 23989 T (Mehnaz et al, 2007b) were analysed under the conditions described by Lin et al (2011Lin et al ( , 2012Lin et al ( , 2013Lin et al ( , 2014Lin et al ( , 2015 and Young et al (2015). For direct comparative analysis, all these strains were grown on NA at 30 8C for 2 days, unless specified otherwise.…”