Azospirillum agricola sp. nov., a nitrogen-fixing species isolated from cultivated soil

Abstract

A polyphasic approach was used to characterize a novel nitrogen-fixing bacterial strain, designated CC-HIH038 T , isolated from cultivated soil in Taiwan. Cells of strain CC-HIH038 T were Gram-stain-negative, facultatively aerobic and spiral-shaped, with motility provided by a single polar flagellum. The 16S rRNA gene sequence analysis of strain CC-HIH038T showed highest sequence similarity to Azospirillum doebereinerae (98.0 %), Azospirillum thiophilum (97.5 %), Azospirillum rugosum (97.4 %) and Azospirillum zeae (97.2 %) and lower sequence similarity (,97.0 %) to all other species of the genus Azospirillum. According to DNA-DNA association, the relatedness values of strain CC-HIH038 T with A. doebereinerae, A. thiophilum, A. rugosum and A. zeae were 51.8 %, 41.2 %, 56.5 % and 37.5 %, respectively. Strain CC-HIH038 T was able to grow at 20-37 8C and pH 7.0-8.0. Strain CC-HIH038 T gave positive amplification for dinitrogen reductase (nifH gene); the activity was recorded as 8.4 nmol ethylene h 21. The predominant quinone system was ubiquinone Q-10 and the DNA G+C content was 68.8 mol%. The major fatty acids found in strain CC-HIH038 T were C 16 : 0 , iso-C 18 : 0 , C 16 : 0 3-OH, C 14 : 0 3-OH/iso-C 16 : 1 and C 18 : 1 v7c/C 18 : 1 v6c. Based on the distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence analysis, strain CC-HIH038

Ora-pro-nobis (Pereskia aculeata Mill.) Nutrition as Related to Soil Chemical and Physical Attributes and Plant Growth-Promoting Microorganisms

“…Azospirillum agricola, another species that occurred in this study, was also isolated in soil cultivated in Taiwan. All the samples studied registered this species, which showed greater similarity to Azospirillum doebereinerae (98.0%), Azospirillum thiophilum (97.5%), Azospirillum rugosum (97.4%), and Azospirillum zeae (97.2%) (Lin et al 2016). However, studies have not yet been developed regarding the efficiency of this species in plant growth promotion.…”

Ureide metabolism in plant-associated bacteria: purine plant-bacteria interactive scenarios under nitrogen deficiency

“…Curiously, the microbial purine pathways from allantoin to oxamate have not been a focus of research despite the use of oxamic acid thiohydrazides as precursors for the formation of hydrazones previously inaccessible from traditional hydrazones chemistry (Volkova et al 2017). Finally, the NH 4 + produced in routes 1 or 2 is broken down to NH 3 in either one-step reaction via urease as described in some rhizobiales bacteria (Neuvonen et al 2016) or in a two-step reaction catalyzed by the biotindependent enzyme urea amidolyase (UAL) complex comprised by the enzymes urea carboxylase and the upregulated gene msmeg_2189 encoded allophanate hydrolase in Acetobacter pasteurianus, Granulibacter bethesdensis, Komagataeibacter nataicola, M. extorquens, M. mesophilicum, M. nodulans, M. oryzae, M. radiotolerans, M. smegmatis, Oleomonas sagaranensis, and S. avermitilis (Lin et al 2016;Minami et al 2016;Petridis et al 2016;Zhang et al 2017a). Genes encoding the enzymes allantoinase, allantoate aminohydrolase, ureidoglycolate lyase, ureidoglycolate dehydrogenase, and urease as well as for the allantoin protoncoupled symporter (pucI, also known as ALLP) and uric acid transporter (pucJK) were described in H. seropedicae (Matiollo et al 2009), B. megaterium, B. guano, B. brevis, B. polymyxa, and B. fastidiosus (except the strain C.4) (Ma et al 2016).…”

Rhizosphere Microorganisms’ Collection of IBPPM RAS: Revision of Azospirillum Strains Based on 16S rRNA Gene Sequence Analysis

“…Их колонии на диагностической малатно-солевой среде мелкие ( 2-3 мм), сухие, полупрозрачные, белые или слегка розовые, похожие на таковые у A. lipoferum, A. brasilense и A. doebereinerae [19]. На среде с конго-красным -красителем, который, как известно, адсорбируется клетками таких видов, как A. brasilense, A. lipoferum, [3]. Для этой цели подходящим и достаточно надежным методом (хотя и имеющим некоторые ограничения) является филогенетический анализ, основанный на сравнении полноразмерных нуклеотидных последовательностей гена 16S рРНК [14,21].…”