Description of Aphelenchoides xui sp. n. (Nematoda: Aphelenchoididae) in packaging wood from South Africa

Abstract

Aphelenchoides xui sp. n. is described and illustrated from imported packaging wood from South Africa intercepted at Ningbo Port, China. The new species has a body length of 564-820 ßm (males) and 549-882 /xm (females). The cuticle is weakly annulated with four lines in the lateral field. The stylet is 11-13 /xm long and has small basal swellings. The excretory pore is located ca one body diam. posterior to the median bulb, or 75-100 /xm from the head. Spicules are relatively large (18-23 /xm) with apex and rostrum rounded and well developed and the end of the dorsal limb clearly curved ventrad like a hook. The male tail bears six (2 -I-2-1-2) caudal papillae. The spermatheca is axial and oblong and contains disc-like sperm. The female tail is conical, terminating in a complicated step-hke projection, usually with many üny nodular protuberances. The new species is morphologically similar to A. arcticus, A. haguei and A. parasaprophilus in Group 2 sensu Shahina, but is distinguished by spicule shape and form of the female tail terminus. Phylogenetic analysis based on SSU and partial LSU sequences revealed that A. xui sp. n. was closest to A. varicaudatus, although some species of Laimaphelenchus and Schistonchus were also included in the same branch.

Description of Aphelenchoides macrospica n. sp. (Nematoda: Aphelenchoididae) from Northwestern Iran

“…is grouped with other genera of Aphelenchoidinae, i.e., Ficophagus Davies, Ye, Kanzaki, Bartholomaeus, Zeng, and Giblin-Davis, 2015, Martininema Davies, Ye, Kanzaki, Bartholomaeus, Zeng, and Giblin-Davis, 2015, Laimaphelenchus Fuchs, 1937, and Schistonchus s.s. Davies, Ye, Kanzaki, Bartholomaeus, Zeng, and Giblin-Davis, 2015, in clade 2. According to our molecular analysis, the members of the genus Aphelenchoides appears to be paraphyletic, a finding that agrees with previous studies (Zhao et al, 2008;Rybarczyk-Mydl À owska et al, 2012;Wang et al, 2013;Fang et al, 2014aFang et al, , 2014bMiraeiz et al, 2015;Esmaeili et al, 2016aEsmaeili et al, , 2016bGolhasan et al, 2016). Furthermore Aphelenchoides, Laimaphelenchus, and Schistonchus s. l cannot be clearly separated, possibly implying that all the genera share a recent common ancestor (Zeng et al, 2007).…”

Description of Aphelenchoides stellatus n. sp. (Nematoda: Aphelenchoididae) found in packaging wood from Japan

“…and kept as cultures or permanent slides for further identification. One isolate from South Africa was identified as Aphelenchoides xui (Wang et al, 2013). The description of the new species enriched our knowledge on Aphelenchoides species having a star-like tail terminus, especially for those wood-dwelling species.…”

“…All exported package materials should be properly heat-or methyl bromide-treated and officially marked in line with ISPM 15 (Wang et al, 2013). However, in recent years, quite a number of living nematodes have been repeatedly detected in Ningbo port from imported packaging wood bearing ISPM marks.…”

“…have been detected (Gu et al, 2006). However, some species belonging to other genera of Aphelenchoi didae have also been detected from imported wood pack aging materials (Gu et al, 2010(Gu et al, , 2013aWang et al, 2013). Some Aphelenchoides species also have been re ported from pine woods in China (Zhuo et al, 2010), in cluding A. besseyi Christie, 1942, which is listed as a reg ulated pest and characterised by a star-like female tail tip (EPPO, 2004).…”

PM 7/39 (2) Aphelenchoides besseyi

“… Aphelenchoides species: (A) female; (B) male; (C) female anterior end; (D) lateral field; (E) female tail; (F) male tail; (G) female tail terminal mucro; and (H) male tail terminal mucro.Source: (A, B, E) Wang et al . (); (D, G) Deimi et al . (); (H) Yu and Tsay (); (C, F) photos courtesy Z. F. Yang and H. Xie, South China Agricultural University, Guangzhou, China).…”