Dermatophytosis in an adult cattle due to Trichophyton verrucosum

Pal, Mahendra

doi:10.15761/ahdvs.1000106

Cited by 13 publications

(12 citation statements)

References 14 publications

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“…As previously reported [19,20], terbinafine and miconazole were effective antifungal drugs for dermatophytes followed by itraconazole and griseofulvin whereas; fluconazole was the least active antifungal agent. Recently, Pal [21] form to the ferrous (Fe +2 ) form because of their reductive capabilities [25]. According to our results, ferric reducing power and TPC content are related to one another.…”

Section: Discussionsupporting

confidence: 56%

Ringworm in calves: Risk factors, improved molecular diagnosis, and efficacy of Aloe vera gel extract for treatment

Tartor¹,

El-Neshwy²,

Merwad³

et al. 2019

Preprint

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Background: Calves dermatophytosis is a major public and veterinary health problem worldwide due to its zoonotic potential and economic losses in cattle farms. However, it has lacked adequate attention; thereby for effective control measures it is worth determining ringworm prevalence, risk factors and direct sample nested-PCR diagnostic indices as compared to conventional methods for dermatophytes identification. Moreover, Aloe vera gel extract (AGE) phenolic composition and its in-vitro and in-vivo anti-dermatophytic activity in comparison to antifungal drugs were evaluated. Results: Of 760 examined calves, 55.79 % showed ringworm lesions. 84.91% were positive for fungal elements in direct microscopy, and 79.72% were culture positive. Trichophyton verrucosum was the most frequently identified dermatophytes (90.24%). Risk of dermatophytosis is high in 4-6 month than 1-month aged calves (60% versus 41%), in summer and winter compared to spring and autumn seasons (66% and 54% versus 48%). Poor hygienic conditions, intensive breeding system, animals raised for meat production, parasitic infestation, crossbreed, and newly purchased animals were statistically significant risk factors correlated with dermatophytosis. One-step PCR targeting conserved regions in the 18S and 28S genes achieved unequivocal identification of T. verrucosum and T. mentagrophytes in hair samples. Nested-PCR achieved an excellent performance in all tested diagnostic indices and increased the species-specific detection of dermatophytes by 20 % as compared to culture. Terbinafine and miconazole were the most active antifungal agents for dermatophytes. Gallic acid, caffeic acid, chlorogenic acid, cinnamic acid, aloe-Emodin, quercetin, and rutin are the major phenolic compounds of AGE identified by High-performance liquid chromatography (HPLC). These compounds increased and synergized the anti-dermatophytic activity of AGE. The treated groups showed significantly lower clinical scores than the control group ( P < 0.05). The calves were successfully treated with topical AGE (500 ppm) resulting in clinical and mycological cure within 14-28 day of the experiment. Conclusions: Implementation of nested-PCR assay providing a rapid diagnostic tool for dermatophytosis augments and complement the conventional methods for initiating targeted treatments of calves ringworm. The recognized anti-dermatophytic potential of AGE is advantageous countenance to commercial drugs to go used in therapeutics.

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Section: Discussionsupporting

confidence: 56%

Ringworm in calves: Risk factors, improved molecular diagnosis, and efficacy of Aloe vera gel extract for treatment

Tartor¹,

El-Neshwy²,

Merwad³

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…As reported previously [ 21 , 22 ], terbinafine and miconazole were effective antifungal drugs for dermatophytes followed by itraconazole and griseofulvin; in contrast, fluconazole was the least-active antifungal agent. Recently, Pal [ 23 ] recommended the performance of further research for the development of cheap, safe, and potent chemotherapeutic agents for cattle dermatophytosis management. AGE is a cheap, easily obtainable, and safe natural product.…”

Section: Discussionmentioning

confidence: 99%

Ringworm in calves: risk factors, improved molecular diagnosis, and therapeutic efficacy of an Aloe vera gel extract

et al. 2020

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Background Dermatophytosis in calves is a major public and veterinary health concern worldwide because of its zoonotic potential and associated economic losses in cattle farms. However, this condition has lacked adequate attention; thus, to develop effective control measures, we determined ringworm prevalence, risk factors, and the direct-sample nested PCR diagnostic indices compared with the conventional methods of dermatophytes identification. Moreover, the phenolic composition of an Aloe vera gel extract (AGE) and its in vitro and in vivo antidermatophytic activity were evaluated and compared with those of antifungal drugs. Results Of the 760 calves examined, 55.79% (424/760) showed ringworm lesions; 84.91% (360/424) were positive for fungal elements in direct-microscopy, and 79.72% (338/424) were positive in culture. Trichophyton verrucosum was the most frequently identified dermatophyte (90.24%). The risk of dermatophytosis was higher in 4–6-month-old vs. 1-month-old calves (60% vs. 41%), and in summer and winter compared with spring and autumn seasons (66 and 54% vs. 48%). Poor hygienic conditions, intensive breeding systems, animal raising for meat production, parasitic infestation, crossbreeding, and newly purchased animals were statistically significant risk factors for dermatophytosis. One-step PCR targeting the conserved regions of the 18S and 28S genes achieved unequivocal identification of T. verrucosum and T. mentagrophytes in hair samples. Nested-PCR exhibited an excellent performance in all tested diagnostic indices and increased the species-specific detection of dermatophytes by 20% compared with culture. Terbinafine and miconazole were the most active antifungal agents for dermatophytes. Gallic acid, caffeic acid, chlorogenic acid, cinnamic acid, aloe-Emodin, quercetin, and rutin were the major phenolic compounds of AGE, as assessed using high-performance liquid chromatography (HPLC). These compounds increased and synergized the antidermatophytic activity of AGE. The treated groups showed significantly lower clinical scores vs. the control group (P < 0.05). The calves were successfully treated with topical AGE (500 ppm), resulting in clinical and mycological cure within 14–28 days of the experiment; however, the recovery was achieved earlier in the topical miconazole 2% and AGE plus oral terbinafine groups. Conclusions The nested PCR assay provided a rapid diagnostic tool for dermatophytosis and complemented the conventional methods for initiating targeted treatments for ringworm in calves. The recognized antidermatophytic potential of AGE is an advantageous addition to the therapeutic outcomes of commercial drugs.

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“…Dermatofitosis adalah mikosis superfisialis yang menyerang kulit, rambut dan kuku manusia dan hewan (Alshawa -et al, 2012). Dermatofitosis sering terjadi pada hewan piara, hewan ternak dan terkadang juga pada satwa liar (Moriello 2014;Pal 2017;Melo et al, 2018;Pereira et al, 2018). Dermatofitosis jarang terjadi pada burung (Chermette et al, 2008).…”

Section: Pendahuluanunclassified

Molecular Technique for Dermatophyte Identification Isolated From Pets in Jakarta and Bogor

et al. 2021

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Dermatophytosis is one of the superficial mycoses which causes skin health problems in pet animals. This study conducted molecular characterization using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) on specimens obtained from patients suspected of dermatophytosis in several clinics in DKI Jakarta Province and Bogor City. Fifty samples of skin scrapings from patients suspected of clinically dermatophytosis were collected and analyzed by conventional and molecular techniques. The Research aimed to identify dermatophyte that were isolated from pet animals using PCR-RFLP technique. The primers of ITS 1, ITS 4, Chytin Synthase, and cutting enzymes of Dde1 were used in this Research. Four specimens off 50 spesimens were tested positive using direct and culture PCR examination techniques. Based on the sequencing results, Microsporum canis was identified in four spesimens. Specimens that were positive for dermatophytes followed by RFLP using the Dde 1 enzyme. The results of the study showed that molecular techniques were a reliable way to determine the high-precision dermatophytes in diagnosing dermatophytosis. The Results also showed that molecular arrangement of B1 isolate was different from three other isolates.

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Dermatophytosis in an adult cattle due to Trichophyton verrucosum

Cited by 13 publications

References 14 publications

Ringworm in calves: Risk factors, improved molecular diagnosis, and efficacy of Aloe vera gel extract for treatment

Ringworm in calves: Risk factors, improved molecular diagnosis, and efficacy of Aloe vera gel extract for treatment

Ringworm in calves: risk factors, improved molecular diagnosis, and therapeutic efficacy of an Aloe vera gel extract

Molecular Technique for Dermatophyte Identification Isolated From Pets in Jakarta and Bogor

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