Derivatization of phenolic acids for capillary gas chromatography with hydrogen flame ionization and electron-capture detection

A Simple Method for Safe Determination of the Activity of Palladium on Activated Carbon Catalysts in the Hydrogenation of Cinnamic Acid to Hydrocinnamic Acid

“…Both compounds have a carboxyl group and a benzene ring. Meanwhile, the presence of a double bond in the side chain in the CA creates a conjugated electron-rich system in which the electron-capture process represents resonance capture that produces a stable negative molecular ion. , Therefore, GC was used to evaluate the peak area of CA for each solution. In this method, the CA and acetonitrile peaks were observed in retention times of 9–9.6 min and 1.5–2 min, respectively.…”

Determination of cinnamic acid and 4‐coumaric acid in alfalfa (Medicago sativa L.) cell suspension cultures by gas chromatography

“…Furthermore, our attempts to resolve cinnamic acid (3-phenylpropenoic acid, CA) from interfering compounds present in alfalfa cell extracts using HPLC have been unsuccessful (Orr et al, 1993); in spite of the good resolution of CA derivatives, a major contaminant co-chromatographed with CA making quantitation of the low levels of CA impossible. Several gas chromatographic (GC) methods for the analysis of phenylpropenoic acids have been reported (Lehtonen and Ketola, 1986;Greenaway et a[., 1991); one method (Greenaway et al, 1991) was useful for the qualitative and semiquantitative analysis Author to whom correspondence should be addressed. Present address: Department of Biological Sciences, University of Durham, Science Laboratories, South Road, Durham, DH1 3HP, UK.…”

Selective sample preparation for determination of 11-nor-9-carboxy-.DELTA.9-tetrahydrocannabinol from human urine by gas chromatography with electron capture detection

“…Selective derivatization of carboxyl and phenolic functionalities on the same organic molecule has been recently reported (8). Under very stringent conditions of temperature (-15 °C), reagent concentrations (minimal excess), and reaction times (0.5 min) the carboxyl moiety of compounds with phenolic and carboxylic acid groups could be selectively esterified with diazomethane and the phenolic group(s) subsequently derivatized with electrophoric reagents preparatory to gas chromatography/electron capture detection (GC/ECD) analysis.…”