A solld support of potystyrene-dlvlnylbenzene cross-linked copolymerlc macroretlcular resln was used to simultaneously adsorb and oxlmate a carbonyl-contalnlng prostaglandln directly from plasma. The process was carrled out at room temperature and was complete M M n 20 mln. Thts technlqua slmpllfled one of the exactlng analytlcal derlvatlzatlons of current methods and substantlalty reduced the time required In sample preparatlon of thls analyte for gas chromatography/mass spectrometry wlth negatlvelon chemlcal Ionlzatbn detecth. The Inveetlgath Mentlfled oxhatlng reagents that produced derlvatlves wlth deslred negatlve-Ion chemical-lonlzatlon-lnduced fragmentatlon. The pH of the aqueous phase In the reactlon mlxture, the physlcal characterlstlcs of these reagents, and aqueous-based derlvatlzatlon of carbonyls on a llpophlllc sterold lndlcated that the site of the reaction was the resln surface.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.