Derivatization of peptides for their determination by chromatographic methods

Koller, Marianne; Eckert, H.

doi:10.1016/s0003-2670(97)00321-8

Cited by 43 publications

(35 citation statements)

References 165 publications

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“…Amino acid analysis by HPLC presents limitations that are specifically related to sensitive and selective detection, in addition to a wide range of wavelengths varying from 205 to 230 nm (32). For this reason, studies related to the development of an adequate HPLC method, aimed to analyze glutamine in different systems, were widely found in the literature.…”

Section: Determination Of Glutamine Content Into Liposomesmentioning

confidence: 99%

Glutamine-Loaded Liposomes: Preliminary Investigation, Characterization, and Evaluation of Neutrophil Viability

et al. 2015

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Abstract. Glutamine has received attention due to its ability to ameliorate the immune system response. Once conventional liposomes are readily recognized and captured by immune system cells, the encapsulation of glutamine into those nanosystems could be an alternative to reduce glutamine dosage and target then to neutrophils. Our goals were to nanoencapsulate glutamine into conventional liposomes (Gln-L), develop an analytical high-performance liquid chromatography (HPLC) method for its quantification, and evaluate the viability of neutrophils treated with Gln-L. Liposomes were prepared using the thin-film hydration technique followed by sonication and characterized according to pH, mean size, zeta potential, and drug encapsulation efficiency (EE%). We also aimed to study the effect of liposomal constituent concentrations on liposomal characteristics. The viability of neutrophils was assessed using flow cytometry after intraperitoneal administration of free glutamine (Gln), Gln-L, unloaded-liposome (UL), and saline solution as control (C) in healthy Wistar rats. The selected liposomal formulation had a mean vesicle size of 114.65±1.82 nm with a polydispersity index of 0.30±0.00, a positive surface charge of 36.30±1.38 mV, and an EE% of 39.49±0.74%. The developed chromatographic method was efficient for the quantification of encapsulated glutamine, with a retention time at 3.8 min. A greater viability was observed in the group treated with glutamine encapsulated compared to the control group (17%), although neutrophils remain viable in all groups. Thus, glutamine encapsulated into liposomes was able to increase the number of viable neutrophils at low doses, thereby representing a promising strategy for the treatment of immunodeficiency conditions.

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Section: Determination Of Glutamine Content Into Liposomesmentioning

confidence: 99%

Glutamine-Loaded Liposomes: Preliminary Investigation, Characterization, and Evaluation of Neutrophil Viability

et al. 2015

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“…These substances often lack from a suitable chromophore, fluorophore or electrophore and have to be detected by the UV-absorption within the wavelength range from 205 to 230 nm. This range, however, is very unspecific because a lot of substances show UV-absorption therein [9,10]. This detection problem could be solved by derivatization procedures which allow transforming amino acids onto detectable forms.…”

Section: Introductionmentioning

confidence: 99%

“…In the post-column derivatization, the analytes are reacted with derivatizing reagent after their HPLC separation and before they reach the detector [9]. The advantage of this process is the previous separation of reaction interfering analytes.…”

Section: Introductionmentioning

confidence: 99%

Validation of a HPLC Method for Determination of Glutamine in Food Additives Using Post-Column Derivatization

Chorilli¹,

Salgado²,

Santos³

et al. 2012

AJAC

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An ion-exchange chromatography (IEC) followed by post-column derivatization method was validated for the determination of glutamine in food additive products. The chromatographic method was carried out on a sodium cation-exchange column and using detection at 570 nm. The mobile phase consisted of sodium eluant, pH 3.15, with 5% sulfolane, sodium eluant, pH 7.40 and sodium column regenerant. The method was linear in the range of 4 -50 µg/mL (r 2 = 0.9999). The accuracy was 99.78%. Moreover, method validation demonstrated acceptable results for precision and robustness. The method was found to be able to use for routine analysis of the glutamine.

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“…Furthermore, current methods have a limited sensitivity because they are based on absorption measurements (4,5). To overcome these limitations, fluorimetric methods using covalent and non-covalent probes have been widely used for the investigation and determination of proteins because they possess high sensitivity and selectivity and provide more information, and their advances for the determination of protein are summarized in some reviews (6)(7)(8). The covalent fluorescent probes suitable for labelling the N-terminus or another functioned group of proteins have been successfully used because of their high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence enhancement effect of the morin–Al³⁺–sodium dodecyl benzene sulphonate–protein system and the determination of proteins

Wang

Yang

et al. 2005

Luminescence

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The fluorescence intensity of the morin-Al(3+) complex was greatly enhanced by proteins in the presence of sodium dodecyl benzene sulphonate (SDBS). Based on this, a new fluorimetric method for the determination of protein was developed. Under optimum conditions, the enhanced intensity of fluorescence was in proportion to the concentration of proteins in the range 1.0 x 10(-8)-1.3 x 10(-5) g/mL for bovine serum albumin (BSA), 4.0 x 10(-8)-1.2 x 10(-5) g/mL for egg albumin (EA) and 5.0 x 10(-8)-1.2 x 10(-5) g/mL for human serum albumin (HSA). Their detection limits (S:N = 3) were 5.0 x 10(-9), 1.8 x 10(-8) and 1.6 x 10(-8) g/mL, respectively. The interaction mechanism was also studied.

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Derivatization of peptides for their determination by chromatographic methods

Cited by 43 publications

References 165 publications

Glutamine-Loaded Liposomes: Preliminary Investigation, Characterization, and Evaluation of Neutrophil Viability

Glutamine-Loaded Liposomes: Preliminary Investigation, Characterization, and Evaluation of Neutrophil Viability

Validation of a HPLC Method for Determination of Glutamine in Food Additives Using Post-Column Derivatization

Fluorescence enhancement effect of the morin–Al³⁺–sodium dodecyl benzene sulphonate–protein system and the determination of proteins

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Derivatization of peptides for their determination by chromatographic methods

Cited by 43 publications

References 165 publications

Glutamine-Loaded Liposomes: Preliminary Investigation, Characterization, and Evaluation of Neutrophil Viability

Glutamine-Loaded Liposomes: Preliminary Investigation, Characterization, and Evaluation of Neutrophil Viability

Validation of a HPLC Method for Determination of Glutamine in Food Additives Using Post-Column Derivatization

Fluorescence enhancement effect of the morin–Al3+–sodium dodecyl benzene sulphonate–protein system and the determination of proteins

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Fluorescence enhancement effect of the morin–Al³⁺–sodium dodecyl benzene sulphonate–protein system and the determination of proteins