Derivatization of alcohols using acridone-9-N-acetyl-benzene-disulfonate as a condensation agent and its application for the determination of volatile alcohols in human plasma by liquid chromatography with fluorescence detection

You, Jing; Sun, Xuejun; Lao, Wenjian; Ou, Qingyu; Jiang, Daozhong

doi:10.1016/s0003-2670(99)00202-0

Cited by 3 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A method for quantification of amino compounds has also been published based on 9-(2-hydroxyethyl)acridone as derivatization reagent after formation of its activated amide intermediate 9-(2-acridone)-oxyethylcarbonylimidazole [21]. Acridone-9-ethylp-toluenesulfonate was applied for determination of free fatty acids and acridone-9-N-acetyl-benzene-disulfonate for determination of alcohols [22]. N-(9-acridinyl)maleimide was originally developed as thiol reagent [23] but utilized more frequently as reagent for sulfite determination [24].…”

Section: Introductionmentioning

confidence: 99%

Determination of plasma aminothiols by high performance liquid chromatography after precolumn derivatization with N-(2-acridonyl)maleimide

Benkova

Lozanov

Ivanov

et al. 2008

Journal of Chromatography B

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Determination of plasma aminothiols by high performance liquid chromatography after precolumn derivatization with N-(2-acridonyl)maleimide

Benkova

Lozanov

Ivanov

et al. 2008

Journal of Chromatography B

View full text Add to dashboard Cite

“…Several studies [14,16,17] have reported that the solvent of the reaction can influence the derivatization of the alcohol. Acetonitrile, acetone, chloroform, dichloromethane, dimethyl sulfoxide, DMF, and toluene were tested as derivatization solvents in this study.…”

Section: Derivatization Solventmentioning

confidence: 99%

New derivatizing reagent for analysis of diethylene glycol by HPLC with fluorescence detection

Jie

Liu

et al. 2008

J of Separation Science

View full text Add to dashboard Cite

N-(2-Phenyl-indolyl)-acetic acid (PIAA), a new fluorescent derivatizing reagent, was used for the determination of diethylene glycol (DEG) by high-performance liquid chromatography with fluorescence detection. DEG was derivatized to ester by using PIAA in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodimide hydrochloride (as dehydrating agent) and 4-(dimethylamino)pyridine (as base catalyst) in acetonitrile at 60 degrees C for 75 min. The influence of solvent, temperature, catalyst base, concentration of labeling reagent, and couple reagent on the derivatization was investigated. The fluorescence detection was performed with excitation at 340 nm and emission at 377 nm. Baseline separation was obtained on an Ultimate XB-C18 analytical column with water/acetonitrile gradient elution, good linearity was obtained within 0.5-50 microg/mL with a correlation coefficient of 0.9997. The limit of detection was 0.01 microg/mL (signal-to-noise ratio = 3). The method has been successfully applied to determine DEG in toothpaste samples with satisfactory recoveries ranging from 89.0 to 94.9%. The proposed method was shown to be a promising technique for the determination of DEG with high sensitivity.

show abstract

“…To improve the detection sensitivity and the selectivity, fluorescence agents, such as p-N,N-dialkylaminotrifluoroacetylstilbenes, 7 4-nitrobenzoyl chloride, 8 acridone-9-Nacetylbenzenedisulfonate, 9 5-(4-aminophneyl)-2-(2-pyrazinyl)-1,3-oxazole, 10 have been applied to the quantification of alcohols in fluorometry as well as in liquid chromatography (LC) with precolumn or postcolumn derivatization techniques. Although these methods are sensitive, they involve rather complicated processes, especially in the derivatization.…”

Section: Introductionmentioning

confidence: 99%

Liquid Chromatographic Determination of Alcohols in Food and Beverages with Indirect Polarimetric Detection Using a β-Cyclodextrin Mobile Phase

et al. 2002

View full text Add to dashboard Cite

An indirect polarimetric detection method for the determination of alcohols has been proposed in liquid chromatography (LC). Optically active mobile-phase additives, such as β-cyclodextrin (β-CD), could be used to visualize optically inactive alcohols in reversed-phase LC. The visualization of alcohols is based on a perturbation of the partition of β-CD caused by the alcohols. The detection limits of the present system at a signal-to-noise ratio of 3 were 0.031, 0.019, 0.018, 0.013, 0.011, 0.008 and 0.008% (v/v) for ethanol, 2-propanol, 1-propanol, 2-methyl-2-propanol, 2-butanol, 2-methyl-1-propanol and 1-butanol, respectively. The method was successfully applied to the determination of ethanol present in food and beverage samples.

show abstract

Derivatization of alcohols using acridone-9-N-acetyl-benzene-disulfonate as a condensation agent and its application for the determination of volatile alcohols in human plasma by liquid chromatography with fluorescence detection

Cited by 3 publications

References 17 publications

Determination of plasma aminothiols by high performance liquid chromatography after precolumn derivatization with N-(2-acridonyl)maleimide

Determination of plasma aminothiols by high performance liquid chromatography after precolumn derivatization with N-(2-acridonyl)maleimide

New derivatizing reagent for analysis of diethylene glycol by HPLC with fluorescence detection

Liquid Chromatographic Determination of Alcohols in Food and Beverages with Indirect Polarimetric Detection Using a β-Cyclodextrin Mobile Phase

Contact Info

Product

Resources

About