Liquid Chromatographic Determination of Alcohols in Food and Beverages with Indirect Polarimetric Detection Using a β-Cyclodextrin Mobile Phase

Abstract

An indirect polarimetric detection method for the determination of alcohols has been proposed in liquid chromatography (LC). Optically active mobile-phase additives, such as β-cyclodextrin (β-CD), could be used to visualize optically inactive alcohols in reversed-phase LC. The visualization of alcohols is based on a perturbation of the partition of β-CD caused by the alcohols. The detection limits of the present system at a signal-to-noise ratio of 3 were 0.031, 0.019, 0.018, 0.013, 0.011, 0.008 and 0.008% (v/v) for ethanol, 2-propanol, 1-propanol, 2-methyl-2-propanol, 2-butanol, 2-methyl-1-propanol and 1-butanol, respectively. The method was successfully applied to the determination of ethanol present in food and beverage samples.

Improvement of Qualitative Analyses of Aliphatic Alcohols Using Direct Catalytic Fuel Cell and Chemometric Analysis Format

“…As is well known, there are many methods for the quantitative and qualitative analysis of alcoholic substances. Leaving aside the oldest chemical assays [24] or densitometric ones used, above all, for ethanol [25], there are quite a few instrumental methods for the determination of alcoholic substances, which are based on gas chromatography [26,27], liquid chromatography, HPLC and ion chromatography [28][29][30], GC-MS [31], and also on colorimetric [32] and spectrophotometric methods (UV-Vis) [33,34], or NIR [35][36][37], and so on. There is no shortage of sensorial methods either [38][39][40].…”

Colorimetric Detection of Aliphatic Alcohols in β-Cyclodextrin Solutions

“…Previous reports on the detection of aliphatic alcohols by colorimetric methods include the use of a single ionic liquid, containing a modified pH indicator, to distinguish between eight aliphatic alcohols 27 as well as copper-containing metal–organic frameworks 28 and iron complexes 29 to accomplish effective detection. Cyclodextrins have also been reported in isolated instances to act as sensors for aliphatic alcohols through the use of cyclodextrin-based stationary phases in chiral chromatography 30 as well as through the use of a quartz crystal microbalance coated with cyclodextrin-derived compounds. 31 To the best of our knowledge, the combination of cyclodextrin-based complexation and colorimetric detection has not been reported to date.…”