Dereplication of Natural Products with Antimicrobial and Anticancer Activity from Brazilian Cyanobacteria

Shishido, Tânia Keiko; Popin, Rafael Vicentini; Jokela, Jouni; Wahlsten, Matti; Fiore, Marli Fátima; Fewer, David P.; Herfindal, Lars; Sivonen, Kaarina

doi:10.3390/toxins12010012

Cited by 29 publications

(25 citation statements)

References 96 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The run in the UPLC consisted of flow rate 0.3 mL min −1 at 40 °C in the following gradient mode: solvent A was 0.1% formic acid diluted in water, while solvent B was 0.1% formic acid in an even mixture of acetonitrile and isopropanol (1:1). The run started from a linear gradient of 95% solvent A and 5% of solvent B and in 5 min the gradient finished to 100% solvent B (0% solvent A) [ 62 ]. A UV–VIS diode-array detector identified the separation of the compounds and a Waters Synapt G2- Si mass spectrometer (Waters, Milford, MA, USA) recorded the mass spectra information.…”

Section: Methodsmentioning

confidence: 99%

“…A UV–VIS diode-array detector identified the separation of the compounds and a Waters Synapt G2- Si mass spectrometer (Waters, Milford, MA, USA) recorded the mass spectra information. Positive and negative resolution modes in the electrospray ionisation (ESI) were used and precursor ions from m / z 100 to 2000 (scan time of 0.1 s) and product ions from m / z 50 to 2000 (scan time of 0.2 s) were recorded [ 62 ]. Other setup conditions consisted of capillary voltage of 1.5 kV in positive and 2.0 kV in negative ionisations; source temperature 120 °C while desolvation occurred at 600 °C; sampling cone 40.0; source offset 80.0; desolvation gas flow 1000 L h −1 ; and nebuliser gas pressure 6.5 bar [ 62 ].…”

Section: Methodsmentioning

confidence: 99%

“…Positive and negative resolution modes in the electrospray ionisation (ESI) were used and precursor ions from m / z 100 to 2000 (scan time of 0.1 s) and product ions from m / z 50 to 2000 (scan time of 0.2 s) were recorded [ 62 ]. Other setup conditions consisted of capillary voltage of 1.5 kV in positive and 2.0 kV in negative ionisations; source temperature 120 °C while desolvation occurred at 600 °C; sampling cone 40.0; source offset 80.0; desolvation gas flow 1000 L h −1 ; and nebuliser gas pressure 6.5 bar [ 62 ]. Leucine–encephalin was used as an internal reference while sodium formate and Ultramark 1621 were used for calibration of the mass detection [ 62 ].…”

Section: Methodsmentioning

confidence: 99%

“…Other setup conditions consisted of capillary voltage of 1.5 kV in positive and 2.0 kV in negative ionisations; source temperature 120 °C while desolvation occurred at 600 °C; sampling cone 40.0; source offset 80.0; desolvation gas flow 1000 L h −1 ; and nebuliser gas pressure 6.5 bar [ 62 ]. Leucine–encephalin was used as an internal reference while sodium formate and Ultramark 1621 were used for calibration of the mass detection [ 62 ].…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Microbial Communities of Cladonia Lichens and Their Biosynthetic Gene Clusters Potentially Encoding Natural Products

et al. 2021

Self Cite

View full text Add to dashboard Cite

Lichens have been widely used in traditional medicine, especially by indigenous communities worldwide. However, their slow growth and difficulties in the isolation of lichen symbionts and associated microbes have hindered the pharmaceutical utilisation of lichen-produced compounds. Advances in high-throughput sequencing techniques now permit detailed investigations of the complex microbial communities formed by fungi, green algae, cyanobacteria, and other bacteria within the lichen thalli. Here, we used amplicon sequencing, shotgun metagenomics, and in silico metabolomics together with compound extractions to study reindeer lichens collected from Southern Finland. Our aim was to evaluate the potential of Cladonia species as sources of novel natural products. We compared the predicted biosynthetic pathways of lichen compounds from isolated genome-sequenced lichen fungi and our environmental samples. Potential biosynthetic genes could then be further used to produce secondary metabolites in more tractable hosts. Furthermore, we detected multiple compounds by metabolite analyses, which revealed connections between the identified biosynthetic gene clusters and their products. Taken together, our results contribute to metagenomic data studies from complex lichen-symbiotic communities and provide valuable new information for use in further biochemical and pharmacological studies.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Microbial Communities of Cladonia Lichens and Their Biosynthetic Gene Clusters Potentially Encoding Natural Products

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…CENA135 contra a bactéria entérica Salmonella typhimurium (SILVA et al, 2014) e o potencial para inibir células do câncer de cólon (CT26), além de atividade moderada contra células cancerígenas do pulmão (3LL) (SILVA-STENICO et al, 2013). Recentemente, a linhagem CENA135 foi detectada induzindo apoptoses à linha celular MOLM-13 da leucemia mieloide aguda (LMA) (SHISHIDO et al, 2019). Em vista que metabólitos secundários de origem ribossômico estariam envolvidos a atividades relacionadas, os agrupamentos gênicos preditos no estudo poderiam estar sintetizando metabólitos responsáveis da atividade antimicrobiana e/ou anticancerígena da linhagem.…”

Section: Orf Função Predita Motivounclassified

Taxonomia, genômica e potencial funcional da cianobactéria >i<Oxynema>/i< sp. CENA135

Castillo¹,

Angélica²

View full text Add to dashboard Cite

e em especial a Fernanda Rios Jacinavicius pela guia, ensinamentos e disponibilidade na realização de análises. Às funcionarias do laboratório Ana Paula Dini Andreote e Renata Beatriz Cruz, pelas distintas atividades que realizam no laboratório e ainda assim me forneceram apoio. Aos colegas do laboratório, amigos, com todo carinho e gratidão para Bruno Evangelista de Souza, Taiane Fernanda Barradas, Endrews Delbaje, Caroline Rosa de Carvalho e Rafael Barty Dextro. Em especial para Juliana dos Santos Costa e Thierry Alexandre Pellegrinetti pelo companherismo e o suporte em momentos cinzentos. A nossa Pós-doutoranda Simone Cotta e às estagiárias Julia Polezi Formagio e Lara Losovoi pela juda recebida. Ao pesquisador Danillo Alvarenga pelos conselhos fornecidos A Guilherme K. Hosaka, pelo amor, amizade, companhia, ensinamentos, apoio inestimável durante minha formação, e pelo Pisco. A sua familia pelas atenções recebidas e as sempre interessantes conversas culturais. A Rodolfo Silva e Fernando Matias, pela amizade, apoio e aventuras neste lindo país. Ao Programa de Pós-Graduação em Microbiologia Agrícola e ao Serviço da Pós-Graduação, em especial a Maria Solizete Granziol Silva e Alexandre Joviniano dos Santos, por todo o auxílio e orientação fornecida. O presente trabalho foi realizado com apoio da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES)-Código de Financiamento 001 ABSTRACT Taxonomy, genomics and functional potential of the cyanobacteria Oxynema sp. CENA135 Oxynema is a derivated genus from the polyphyletic Phormidium and with few registered species, which were found in mangroves, marine or high salinity environments. The taxonomic and genomic informations are almost nonexisting for this genus, with a few exceptions of 16S rRNA and 16S-23S ITS sequences available at databases. Previous studies performed with Oxynema sp. CENA135 isolated from a Brazilian mangrove showed its potential to synthesize siderophores, aliphatic hydrocarbons, anticancer and antibiotic substances, besides its effective degradation of some types of textile dyes. In this work, the strain Oxynema sp. CENA135 was analyzed under a polyphasic approach (morphology, habitat, phylogenomics, phylogeny of the 16S rRNA gene and secondary structure of 16S-23S ITS), including the search for genes or gene clusters related to the biosynthesis of natural products and the detection of associated bacteria. After morphological characterization using an optic and a scanning microscopes, the genomic sequencing was performed from a mate pair library at the HiSeq (Illumina) platform. This is the first genome of the genus Oxynema and, therefore, for its assembly it was used a de novo method resulting in 11 scaffolds in a total size of 6.2 Mbp, with N50 of 3,591,108 pb, GC content of 51.6% and average coverage of 190x. The checkM software estimated a high completeness of the genome (99.29%) and few contaminations (1.22%). Genome mining revealed a biorremediation potential due to the presence of genes related to resistance of copper,...

show abstract

In vivo and in silico screening for antimicrobial compounds from cyanobacteria

2022

View full text Add to dashboard Cite

Due to the emerging rise of multi‐drug resistant bacteria, the discovery of novel antibiotics is of high scientific interest. Through their high chemodiversity of bioactive secondary metabolites, cyanobacteria have proven to be promising microorganisms for the discovery of antibacterial compounds. These aspects make appropriate antibacterial screening approaches for cyanobacteria crucial. Up to date, screenings are mostly carried out using a phenotypic methodology, consisting of cyanobacterial cultivation, extraction, and inhibitory assays. However, the parameters of these methods highly vary within the literature. Therefore, the common choices of parameters and inhibitory assays are summarized in this review. Nevertheless, less frequently used method variants are highlighted, which lead to hits from antimicrobial compounds. In addition to the considerations of phenotypic methods, this study provides an overview of developments in the genome‐based screening area, be it in vivo using PCR technique or in silico using the recent genome‐mining method. Though, up to date, these techniques are not applied as much as phenotypic screening.

show abstract

Dereplication of Natural Products with Antimicrobial and Anticancer Activity from Brazilian Cyanobacteria

Cited by 29 publications

References 96 publications

Microbial Communities of Cladonia Lichens and Their Biosynthetic Gene Clusters Potentially Encoding Natural Products

Microbial Communities of Cladonia Lichens and Their Biosynthetic Gene Clusters Potentially Encoding Natural Products

Taxonomia, genômica e potencial funcional da cianobactéria >i<Oxynema>/i< sp. CENA135

In vivo and in silico screening for antimicrobial compounds from cyanobacteria

Contact Info

Product

Resources

About