In vivo and in silico screening for antimicrobial compounds from cyanobacteria

Abstract

Due to the emerging rise of multi‐drug resistant bacteria, the discovery of novel antibiotics is of high scientific interest. Through their high chemodiversity of bioactive secondary metabolites, cyanobacteria have proven to be promising microorganisms for the discovery of antibacterial compounds. These aspects make appropriate antibacterial screening approaches for cyanobacteria crucial. Up to date, screenings are mostly carried out using a phenotypic methodology, consisting of cyanobacterial cultivation, extraction, and inhibitory assays. However, the parameters of these methods highly vary within the literature. Therefore, the common choices of parameters and inhibitory assays are summarized in this review. Nevertheless, less frequently used method variants are highlighted, which lead to hits from antimicrobial compounds. In addition to the considerations of phenotypic methods, this study provides an overview of developments in the genome‐based screening area, be it in vivo using PCR technique or in silico using the recent genome‐mining method. Though, up to date, these techniques are not applied as much as phenotypic screening.

A Novel Application of Laser in Biocontrol of Plant Pathogenic Bacteria

“…With regard to the molecular basis of bioactive compound production from cyanobacteria in general and Syenchococcus sp. in particular, Strieth et al [47] recommended the use of in silico alongside in vivo screening for antimicrobial compounds from cyanobacteria. Indeed, Konstantinou et al [48] screened marine cyanobacteria associated with sponge and found that three marine Syechococcus sp.…”

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“…This halogenase was investigated to uncover its natural substrates [76]. Other studies resulted in advances in the screening of cyanobacterial genomes, whether in vivo through PCR or in silico using genome-mining techniques [77].…”

Nachhaltigkeit in der Bioverfahrenstechnik

“…Die Biomasse an sich kann dabei als Nahrungs(erga ¨nzungs)mittel [3], als Tierfutter oder Du ¨ngemittel dienen [4,5]. Weiterhin produzieren viele Cyanobakterien antimikrobielle Verbindungen, die potenziell neue Wirkmechanismen aufweisen und so zur Beka ¨mpfung von multiresistenten Keimen beitragen ko ¨nnten [6]. Ich bescha ¨ftige mich vor allem mit dem Potenzial terrestrischer Cyanobakterien, die neben CO 2 auch Stickstoff und Feinstaub binden und unter Rauchgas kultiviert werden ko ¨nnen [7].…”