Depressed expression of FAE1 and FAD2 genes modifies fatty acid profiles and storage compounds accumulation in Brassica napus seeds

Shi, Jianxin; Lang, Chunxiu; Wang, Fulin; Wu, Xiao‐Tao; Liu, Renhu; Zheng, Tao; Zhang, Dongqing; Chen, Jinqing; Guan-ting, WU

doi:10.1016/j.plantsci.2017.07.014

Cited by 46 publications

(41 citation statements)

References 42 publications

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“…To date, most high oleic acid germplasms reported are based on the mutation of FAD2 genes in Brassica species (Long et al, 2018;Peng et al, 2010;Wells et al, 2014). Such a narrow gene resource may lead to decreased genetic variation and thus compromise the improvement of yield and stress resistance, as illustrated in previous studies (Bai et al, 2018;Dar et al, 2017;Kinney, 1994;Miquel, 1994;Shi et al, 2017). The problem has also been noted in other oil crops, such as peanut and maize (Belo et al, 2008;Janila et al, 2016;Mikkilineni and Rocheford, 2003;Wang et al, 2015Wang et al, , 2018.…”

Section: Discussionmentioning

confidence: 99%

A novel quantitative trait locus on chromosome A9 controlling oleic acid content in Brassica napus

Zhao

Cai

et al. 2019

Plant Biotechnology Journal

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Summary One of the most important goals in the breeding of oilseed crops, including Brassica napus, is to improve the quality of edible vegetable oil, which is mainly determined by the seed fatty acid composition, particularly the C18:1 content. Previous studies have indicated that the C18:1 content is a polygenic trait, and no stable quantitative trait loci (QTLs) except for FAD2 have been reported. By performing a GWAS using 375 low erucic acid B. napus accessions genotyped with the Brassica 60K SNP array and constructing a high‐density SNP‐based genetic map of a 150 DH population, we identified a novel QTL on the A9 chromosome. The novel locus could explain 11.25%, 5.72% and 6.29% of phenotypic variation during three consecutive seasons and increased the C18:1 content by approximately 3%–5%. By fine mapping and gene expression analysis, we found three potential candidate genes and verified the fatty acids in a homologous gene mutant of Arabidopsis. A metal ion‐binding protein was found to be the most likely candidate gene in the region. Thus, the C18:1 content can be further increased to about 80% with this novel locus together with FAD2 mutant allele without compromise of agronomic performance. A closely linked marker, BnA129, for this novel QTL (OLEA9) was developed so that we can effectively identify materials with high C18:1 content at an early growth stage by marker‐assisted selection. Our results may also provide new insight for understanding the complex genetic mechanism of fatty acid metabolism.

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Section: Discussionmentioning

confidence: 99%

A novel quantitative trait locus on chromosome A9 controlling oleic acid content in Brassica napus

Zhao

Cai

et al. 2019

Plant Biotechnology Journal

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“…FAD2 and FAD6 are expressed in seedlings of Arabidopsis ( Arabidopsis thaliana ) under salinity stress (Feng et al, 2017 ). Down-regulated expression of FATTY ACID ELONGATION 1 ( FAE1 ) and FAD2 modifies the fatty acid profile and accumulated storage compounds in Brassica napus seeds (Shi et al, 2017 ). The ω-3 desaturases comprise FAD3, FAD7, and FAD8, which synthesize 18:3 from 18:2 in the ER (FAD3) and plastids (FAD7 and FAD8), respectively (Gibson et al, 1994 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and Physiological Evidence for the Relationship between Unsaturated Fatty Acid and Salt Stress in Peanut

Sui¹,

Wang²,

Liu³

et al. 2018

Front. Plant Sci.

125

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Peanut (Arachis hypogaea L.) is one of the five major oilseed crops cultivated worldwide. Salt stress is a common adverse condition for the growth of this crop in many countries and regions. In this study, physiological parameters and transcriptome profiles of peanut seedlings exposed to salt stress (250 mM NaCl for 4 days, S4) and recovery for 3 days (when transferred to standard conditions for 3 days, R3) were analyzed to detect genes associated with salt stress and recovery in peanut. We observed that the quantum yield of PSII electron transport (ΦPSII) and the maximal photochemical efficiency of PSII (Fv/Fm) decreased in S4 compared with the control, and increased in R3 compared with those in S4. Seedling fresh weight, dry weight and PSI oxidoreductive activity (ΔI/Io) were inhibited in S4 and did not recover in R3. Superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities decreased in S4 and increased in R3, whereas superoxide anion (O2-•) and hydrogen peroxide (H2O2) contents increased in S4 and decreased in R3. Transcriptome analysis revealed 1,742 differentially expressed genes (DEGs) under salt stress and 390 DEGs under recovery. Among these DEGs, two DEGs encoding ω-3 fatty acid desaturase that synthesized linolenic acid (18:3) from linoleic acid (18:2) were down-regulated in S4 and up-regulated in R3. Furthermore, ω-3 fatty acid desaturase activity decreased under salt stress and increased under recovery. Consistent with this result, 18:3 content decreased under salt stress and increased under recovery compared with that under salt treatment. In conclusion, salt stress markedly changed the activity of ω-3 fatty acid desaturase and fatty acid composition. The findings provide novel insights for the improvement of salt tolerance in peanut.

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“…The improvement of seed quality is one of the most important targets in Brassica breeding for satisfying future edible oil requirements. The functional and nutritional values of different vegetable oils are dependent on the nature of the different fatty acids, which are incorporated into the lipids in lipid phase (triacylglycerols) Oilseed rape represents an excellent cash crop, notably for developing countries where the by‐product of oils processing, a high quality meal, is used as animal feed …”

Section: Summary and Future Issues To Be Resolvedmentioning

confidence: 99%

Current Research Developments on the Processing and Improvement of the Nutritional Quality of Rapeseed (Brassica napus L.)

Beszterda

Nogala‐Kałucka

2019

Euro J Lipid Sci & Tech

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Brassica napus is cultivated largely as a winter or semi‐winter form in Europe and Asia, respectively, whereas spring‐sown rape types are more adapted to the climatic conditions in Canada, northern Europe, and Australia. This plant, the third most important oil crop in the world after maize and oil palm, has had a unique history among those crops that have been targets of breeding and genetic modification. Although agronomy and genetic manipulations of B. napus plants to create seed with substantially no erucic acid content in the seed oil and meal largely free from aliphatic glucosinolates were major breakthroughs, opportunities for further advances in the reduction of antinutritive compounds/enhancement of bioactive compounds, in oil and meal health safety and quality, as well as in agronomic performance and pest resistance, are still greater today than they have ever been. Understanding and application of genetic control for key agronomic traits in B. napus, such as seed oil composition and concentration as well as their environmental interactions, are the main targets, which will underpin the development of the crop for cultivation in an extensive range of environments. It is also needed to improve phytochemical content through traditional breeding programs or through bioengineering of the phenylpropanoid pathway, leading to desirable secondary metabolite accumulation. This paper is a review of modifications (in the field of biotechnology, agronomy, and processing) that have been applied to improve the nutritional value of B. napus L. Practical Applications: This paper has value for people starting their scientific work in the field related to the B. napus biotechnology and nutritional value of vegetable oils. In addition, it is a current compedium of knowledge for people working professionally in the oil industry. Moreover, this article is a cross‐section of the current achievements of genetic engineering in improving the composition of rapeseed and indicates key publications on this subject. Brassica napus L., the third most important oil crop in the world after maize and oil palm, has a unique history among those crops that are targets of breeding and genetic modification. Effective tools of processing, biotechnology, and agronomy are essential to produce rapeseed oil with a high amount of bioactive compounds and continue to be employed in strengthening the product versatility.

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Depressed expression of FAE1 and FAD2 genes modifies fatty acid profiles and storage compounds accumulation in Brassica napus seeds

Cited by 46 publications

References 42 publications

A novel quantitative trait locus on chromosome A9 controlling oleic acid content in Brassica napus

A novel quantitative trait locus on chromosome A9 controlling oleic acid content in Brassica napus

Transcriptomic and Physiological Evidence for the Relationship between Unsaturated Fatty Acid and Salt Stress in Peanut

Current Research Developments on the Processing and Improvement of the Nutritional Quality of Rapeseed (Brassica napus L.)

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