Deposition of superparamagnetic nanohydroxyapatite on iron–fibrin substrates: Preparation, characterization, cytocompatibility and bioactivity studies

Vedakumari, Weslen S.; Priya, Vishnu M.; Sastry, T. P.

doi:10.1016/j.colsurfb.2014.04.021

Cited by 19 publications

(14 citation statements)

References 34 publications

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“…All samples showed no haemolytic activity, since the percentage of haemolysis was less than 2%. These results suggested that CB and CBCa samples can be used as biomaterials [30]. These results are in agreement with literature, as HAp-based materials have already been reported to be haemocompatible [26].…”

Section: Samplesupporting

confidence: 88%

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Hydroxyapatite-based materials of marine origin: A bioactivity and sintering study

Piccirillo

Pullar

Costa

et al. 2015

Materials Science and Engineering: C

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Single phase hydroxyapatite (HAp) and biphasic material hydroxyapatite/β-tricalcium phosphate (HAp/β-TCP) were obtained from a marine source (Atlantic cod fish bones). Here we report a study on the biological properties of these materials, including cytotoxicity, bioactivity and haemocompatibility. Results showed that the materials are not cytotoxic, neither in their powder nor in pellet form; indeed growth of Saos-2 cells was comparable to that of commercial. The haemolysis rate was lower than 2%; hence the materials can be classified as non-haemolytic. Moreover, when immersed in Simulated Body Fluid (SBF), crystal formation was observed on the surface of both materials. The sintering behaviour of the samples was also studied; both powders showed very high sinterability (density higher than 95% of the theoretical value). Overall, these results confirm the suitability of these materials for biomedical applications.

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Section: Samplesupporting

confidence: 88%

“…Other calcium phosphates are also employed in this field, often combined with HAp; tricalcium phosphate (Ca 3 (PO 4 ) 2 , TCP), for instance, shows good biocompatibility and resorbability in both its α and β forms [9]. HAp and other phosphate-based materials are also bioactive, promoting bone growth and formation [30].…”

Section: Introductionmentioning

confidence: 99%

Hydroxyapatite-based materials of marine origin: A bioactivity and sintering study

Piccirillo

Pullar

Costa

et al. 2015

Materials Science and Engineering: C

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“…A high expression of the OPN gene occurs in the stage of bone mineralization [ 37 ]. Several studies point out that CNTs aid in cell adhesion, which is directly related to the expression of the OPN gene [ 30 , 38 , 39 ]. The groups containing the HAp/CNT 1%, HAp/CNT 3% samples showed a significant increase in the expression of the alkaline phosphatase (ALP) gene when compared to the control group * p > 0.05 ( Figure 3 c).…”

Section: Discussionmentioning

confidence: 99%

In Vitro Osteogenesis Stimulation via Nano-Hydroxyapatite/Carbon Nanotube Thin Films on Biomedical Stainless Steel

et al. 2018

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We evaluated the electrophoretic deposition of nanohydroxyapatite/superhydrop hilic multiwalled carbon nanotube composites (nHAp/MWCNT) onto stainless steel biomedical alloys for applications in bone tissue engineering. First, nHAp/MWCNT composites were dispersed into 0.042 mol·L−1 of Ca(NO3)2·4H2O + 0.025 mol·L−1 NH4H2PO4 electrolytes (pH = 4.8) at two different concentrations. Next, a voltage of −2 V was applied using 316L stainless steel as a working electrode (0.27 cm2), a high-purity platinum coil wire was used as the auxiliary electrode, and an Ag/AgCl (3 M) electrode was used as the reference electrode. The nHAp/MWCNT composites were characterized by transmission electron microscopy. The deposited nHAp and nHAp/MWCNT films were characterized by profilometry, scanning electron microscopy, X-ray diffractometry and Raman spectroscopy. Human osteoblast cells were cultivated with the different materials and in vitro cytotoxicity was evaluated using lactate dehydrogenase (LDH) assay. The osteogenesis process was evaluated by mRNA levels of the three genes that are directly related to bone repair: Alkaline Phosphatase, Osteopontin and Osteocalcin. We showed that rough, crystalline apatite thin films containing phases of nHAp were successfully deposited onto 316L stainless steel alloys. Also, we noticed that nHAp/MWCNT thin films deposited onto 316L stainless steel alloys upregulated the expression of important genes related to bone mineralization and maturation. Our results strongly support the possibility of this new alternative to modify the surface of metallic biomedical alloys to promote bone tissue regeneration.

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“…When an MG-63 cell line was cultured in the presence of this composite, its osteogenic potential improved as noted by the upregulated expression of osteogenic genes and enhanced calcium deposition and mineralization. 195 In this manner, as one can easily determine, the efficacy of fibrin was improved through its incorporation with nanoscaffolds and nanomaterials. It has been speculated that such nanomaterials improve the bioactivity of fibrin and availability of fibrin to interact with cells.…”

Section: Application Of Nanotechnology In Fibrin-based Bone Tissue Enmentioning

confidence: 99%

A review of fibrin and fibrin composites for bone tissue engineering

Noori

Ashrafi

Vaez-Ghaemi

et al. 2017

IJN

365

256

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Tissue engineering has emerged as a new treatment approach for bone repair and regeneration seeking to address limitations associated with current therapies, such as autologous bone grafting. While many bone tissue engineering approaches have traditionally focused on synthetic materials (such as polymers or hydrogels), there has been a lot of excitement surrounding the use of natural materials due to their biologically inspired properties. Fibrin is a natural scaffold formed following tissue injury that initiates hemostasis and provides the initial matrix useful for cell adhesion, migration, proliferation, and differentiation. Fibrin has captured the interest of bone tissue engineers due to its excellent biocompatibility, controllable biodegradability, and ability to deliver cells and biomolecules. Fibrin is particularly appealing because its precursors, fibrinogen, and thrombin, which can be derived from the patient’s own blood, enable the fabrication of completely autologous scaffolds. In this article, we highlight the unique properties of fibrin as a scaffolding material to treat bone defects. Moreover, we emphasize its role in bone tissue engineering nanocomposites where approaches further emulate the natural nanostructured features of bone when using fibrin and other nanomaterials. We also review the preparation methods of fibrin glue and then discuss a wide range of fibrin applications in bone tissue engineering. These include the delivery of cells and/or biomolecules to a defect site, distributing cells, and/or growth factors throughout other pre-formed scaffolds and enhancing the physical as well as biological properties of other biomaterials. Thoughts on the future direction of fibrin research for bone tissue engineering are also presented. In the future, the development of fibrin precursors as recombinant proteins will solve problems associated with using multiple or single-donor fibrin glue, and the combination of nanomaterials that allow for the incorporation of biomolecules with fibrin will significantly improve the efficacy of fibrin for numerous bone tissue engineering applications.

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Deposition of superparamagnetic nanohydroxyapatite on iron–fibrin substrates: Preparation, characterization, cytocompatibility and bioactivity studies

Cited by 19 publications

References 34 publications

Hydroxyapatite-based materials of marine origin: A bioactivity and sintering study

Hydroxyapatite-based materials of marine origin: A bioactivity and sintering study

In Vitro Osteogenesis Stimulation via Nano-Hydroxyapatite/Carbon Nanotube Thin Films on Biomedical Stainless Steel

A review of fibrin and fibrin composites for bone tissue engineering

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