Depolymerization reactions of hyaluronic acid in solution

Reháková, Milena; Bakoš, Dušan; Soldán, Maroš; Vizárová, Katarína

doi:10.1016/0141-8130(94)90037-x

Cited by 65 publications

(42 citation statements)

References 8 publications

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“…In this study, to prepare collagen modified by HA, two synergetic methods were selected: dehydrothermal pretreatment and the reaction with water soluble 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide with the addition of N-hydroxysulfosuccinimide. These two ways are preferable for collagen [20,21] and collagen-HA [13][14][15][16] cross-linking since the products exhibit no toxicity towards cells. Besides, DHT cross-linking stabilizes collagen macromolecule and prevents matrix collapse [22].…”

Section: Resultsmentioning

confidence: 98%

“…This seems obvious to indicate that the rheological behavior and thermal stability of scaffold will determine its resistance to contraction. Although a number of investigations on collagen-HA scaffold materials have been done to characterize their biochemical and biological properties [13][14][15][16], the effect of temperature on the conformational and mechanical properties are not clearly elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Changes in denaturation and rheological properties of collagen–hyaluronic acid scaffolds as a result of temperature dependencies

Pietrucha¹

2005

International Journal of Biological Macromolecules

133

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Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Changes in denaturation and rheological properties of collagen–hyaluronic acid scaffolds as a result of temperature dependencies

Pietrucha¹

2005

International Journal of Biological Macromolecules

133

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“…Previous studies have demonstrated that in addition to hematopoietic ablation, whole body irradiation sharply decreases the concentration of glycosaminoglycans, including HA, in mouse spleen and bone marrow (61). During exposure to irradiation, HA undergoes chemical degradation and depolymerization (62). Irradiation induces HA chain fragmentation, and dose-dependently affects the three-dimensional polymeric structure of HA (63,64).…”

Section: Discussionmentioning

confidence: 99%

Hyaluronan Expressed by the Hematopoietic Microenvironment Is Required for Bone Marrow Hematopoiesis

Goncharova

Serobyan

Iizuka

et al. 2012

Journal of Biological Chemistry

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Background: Hyaluronan (HA) contributes to the extracellular matrix in bone marrow. Results: HA expressed by the hematopoietic microenvironment supports hematopoiesis and is involved in hematopoietic stem/progenitor cell migration by regulating the production of soluble factors. Conclusion: Endogenous HA is an important regulatory element of the hematopoietic microenvironment. Significance: Understanding the biology of HA may help to develop strategies for improving the quality of the stem cell microenvironment.

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“…thermal, 2,4) mechanical 2,5) and chemical actions. 6,7) Previously, we described HA depolymerization by phenothiazines and sulfacetamide with UV irradiation.…”

mentioning

confidence: 99%

Hyaluronate Depolymerization Following Thermal Decomposition of Oxytetracycline.

Miyazaki¹,

Yomota²,

Okada³

2001

CHEMICAL & PHARMACEUTICAL BULLETIN

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Hyaluronate (HA) is a glycosaminoglycan ubiquitously distributed in organisms. It has a linear structure without side chains, and a molecular weight of 10 4 to 10 7 . The huge molecular mass characterizes the physiologic and physicochemical properties of HA. High-molecular-weight HA forms entangled networks, having unique viscoelasticity and waterholding ability.1) The characteristics are molecular-weightsensitive, and yet, high molecular weight HA is easily depolymerized by various physical and chemical factors. There are many reports about HA depolymerization by photo, 2,3) thermal, 2,4) mechanical 2,5) and chemical actions. 6,7) Previously, we described HA depolymerization by phenothiazines and sulfacetamide with UV irradiation. 7) These drugs are known to cause dermatological or ophthalmic phototoxicity in patients receiving them in the long term. We have revealed the hydroxyl radicals and hydrated electrons generated during photodegradation of the drugs to be the active chemicals for HA depolymerization.Tetracyclines have also been reported to exhibit phototoxic and photoallergic reactions, with certain radicals proposed to account for in vivo and in vitro toxicity. 8,9) In this paper, the depolymerization of HA by tetracyclines, expressly oxytetracycline (OTC), was investigated. From depolymerization profiles under various conditions, the probable causative derivative was identified. ExperimentalMaterials Two sodium HA samples were used: one was extracted from culture medium of Streptococcus equi, with a weight average molecular weight (M w ) of 2.6ϫ10 6 , and the other was isolated from rooster comb, with a M w of 1.1ϫ106 . Sodium alginate of medium viscosity from kelp was a commercial product of Sigma Chemical Co. Tetracycline hydrochlorides were purchased from Sigma Chemical Co. or Wako Chemical Co. They were used without further purification. Their chemical structures are listed in Table 1. Other reagents were of analytical grade. All the water used was distilled once and then Millipore filtered.Sample Preparation Polymer solutions were mixed with tetracycline solutions to make at final concentrations of 0.1% in 0.2 M NaCl. They were incubated in test tubes in a thermostated water bath. At the desired time, a portion was removed, appropriately diluted and after drugs were eliminated by solid-phase extraction on Sep-Pak C 18 cartridges, the molecular weight was measured. For studies under anaerobic conditions, glass ampules were used in place of test tubes. Solutions in ampules were de-aerated by 10-min nitrogen bubbling, immediately before the necks of the ampules were sealed with a burner. For UV irradiation, lamps (100 V, 5 W) with 254 nm or 365 nm for TLC spot detection were employed. The distance of the vessels from the light source was set at 5 cm. In the experiments with 254 nm irradiation, the vessels were squared quartz cells.Molecular Weight Measurement M w of HA was measured using a size exclusion chromatography (SEC) equipped with a low angle laser light scattering (LALLS). SEC-LALLS (Tosoh ...

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Depolymerization reactions of hyaluronic acid in solution

Cited by 65 publications

References 8 publications

Changes in denaturation and rheological properties of collagen–hyaluronic acid scaffolds as a result of temperature dependencies

Changes in denaturation and rheological properties of collagen–hyaluronic acid scaffolds as a result of temperature dependencies

Hyaluronan Expressed by the Hematopoietic Microenvironment Is Required for Bone Marrow Hematopoiesis

Hyaluronate Depolymerization Following Thermal Decomposition of Oxytetracycline.

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