Changes in denaturation and rheological properties of collagen–hyaluronic acid scaffolds as a result of temperature dependencies

Pietrucha, Krystyna

doi:10.1016/j.ijbiomac.2005.07.004

Cited by 131 publications

(82 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The endothermic peak between 50 and 1608C was due to the evaporation of loosely bonded water. 16,28,29 The sharp maximum at 1128C also indicated that the mixture was of the homogeneous nature despite of the presence of the competition of Col gelation and complexation with Chi in the present study. The exothermic peak at higher temperature (around 3008C) was related to the thermal decomposition of Col and Chi.…”

supporting

confidence: 55%

Synthesis and evaluation of collagen–chitosan–hydroxyapatite nanocomposites for bone grafting

Wang

Tan

et al. 2008

J Biomedical Materials Res

View full text Add to dashboard Cite

Incorporation of hydroxyapatite (HA) into the matrix of collagen (Col) and chitosan (Chi) by in situ synthesis was introduced to prepare nanocomposites. Structural investigations of the pure Col-Chi mixture validated the influence of Chi on Col assembly, but the molecular interactions between Col and Chi was partially depressed during the intervention of in situ HA synthesis, as revealed by FTIR and DSC analyses. A series of Col-Chi-HA (CCHA) nanocomposites with varying HA content were thereby prepared by a sequential method, involving in situ synthesis in the Col-Chi system, then gelling at 25 degrees C and subsequently washing the resultant elastic gel followed by dehydration consolidation. The structural characteristics and biological properties of the dehydrated CCHA nanocomposites were further evaluated by using XRD, FTIR, TG, and SEM analyses and the osteoblast culture experiment. Formation of a well integrated microstructure of organic fibers (ca. 90 nm in size) and dense matrix including inorganic aggregates (less than 30 nm in size) was found in these nanocomposites. Rat Ros 17/2.8 Osteoblasts proliferated and attached well on the surface of both CCHA nanocomposite and Col-Chi mixture. These results indicated that in situ HA synthesis in the Col-Chi system provided a feasible route for bone grafting nanocomposites.

show abstract

supporting

confidence: 55%

Synthesis and evaluation of collagen–chitosan–hydroxyapatite nanocomposites for bone grafting

Wang

Tan

et al. 2008

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…Figure 5 shows the DSC thermographs of collagen and collagen/DAS cryogels with different weight ratios. The broad endothermal peak of pure collagen from %30 to 160 8C, associated with the transformation from crystalline triple helix to amorphous random coil, [37] indicates that collagen is somewhat hydrated. It can be seen that the addition of DAS causes denaturation temperature (T d ) to increase 10-15 8C.…”

Section: Formation and Characterization Of The Cryogelmentioning

confidence: 99%

Collagen Cryogel Cross‐Linked by Dialdehyde Starch

Liu

Cheng

et al. 2010

Macro Materials & Eng

116

View full text Add to dashboard Cite

A 3D spongy collagen cryogel was prepared using DAS as the cross‐linker. FTIR and CD studies demonstrate that crosslinking is achieved through the reaction of the DAS aldehyde groups with the free amino groups in collagen without affecting the triple helix of collagen. SEM demonstrates that the cryogel has a heteroporous structure with interconnecting pores. DSC measurements reveal that the cryogels have improved thermal stability in comparison with pure collagen. Moreover, the ESR shows that the water uptake of the cryogel decreases with DAS content. Evaporation tests indicate that the cryogel can hold moisture for a long time. Since both collagen and DAS are nontoxic and the resultant cryogel is blood‐compatible, the cryogel is expected to be useful, e.g., as wound dressing.magnified image

show abstract

“…4 HA, a component of the glycosaminoglycans (GAGs) in the extracellular matrix (ECMs), is biocompatible, biodegradable, and performs important biological functions such as stabilizing and organizing the ECM, regulating cell adhesion and mobility, and mediating cell proliferation and differentiation. [8][9][10]27,28 Recent biomedical applications of HA included scaffolds for wound healing and tissue engineering, as well as ophthalmic surgery, arthritis treatment, and components for implant materials. [8][9][10]27,28 The objective of this study is to prepare the comb-type PNIPAAm-grafted HA copolymers and to then investigate the feasibility of temperature-sensitive injectable drug carriers.…”

Section: Introductionmentioning

confidence: 99%

Preparation of thermo-responsive and injectable hydrogels based on hyaluronic acid and poly(N-isopropylacrylamide) and their drug release behaviors

et al. 2006

View full text Add to dashboard Cite

Copolymers composed of hyaluronic acid (HA) and poly(N-isopropylacrylamide) (PNIPAAm) were prepared to create temperature-sensitive injectable gels for use in controlled drug delivery applications. Semi-telechelic PNIPAAm, with amino groups at the end of each main chain, was synthesized by radical polymerization using 2-aminoethanethiol hydrochloride (AESH) as the chain transfer agent, and was then grafted onto the carboxyl groups of HA using carbodiimide chemistry. The result of the thermo-optical analysis revealed that the phase transition of the PNIPAAm-grafted HA solution occurred at around 30~33 o C. As the graft yield of PNIPAAm onto the HA backbone increased, the HA-g-PNIPAAm copolymer solution exhibited sharper phase transition. The short chain PNIPAAm-grafted HA (M w = 6,100) showed a narrower temperature range for optical turbidity changes than the long chain PNIPAAm-grafted HA (M w = 13,100). PNIPAAm-grafted HA exhibited an increase in viscosity above 35 o C, thus allowing the gels to maintain their shape for 24 h after in vivo administration. From the in vitro riboflavin release study, the HA-g-PNIPAAm gel showed a more sustained release behavior when the grafting yield of PNIPAAm onto the HA backbone was increased. In addition, BSA released from the PNIPAAm-g-HA gels showed a maximum concentration in the blood 12 h after being injected into the dorsal surface of a rabbit, followed by a sustained release profile after 60 h.

show abstract

Changes in denaturation and rheological properties of collagen–hyaluronic acid scaffolds as a result of temperature dependencies

Cited by 131 publications

References 28 publications

Synthesis and evaluation of collagen–chitosan–hydroxyapatite nanocomposites for bone grafting

Synthesis and evaluation of collagen–chitosan–hydroxyapatite nanocomposites for bone grafting

Collagen Cryogel Cross‐Linked by Dialdehyde Starch

Preparation of thermo-responsive and injectable hydrogels based on hyaluronic acid and poly(N-isopropylacrylamide) and their drug release behaviors

Contact Info

Product

Resources

About