Synthesis and evaluation of collagen–chitosan–hydroxyapatite nanocomposites for bone grafting

Abstract

Incorporation of hydroxyapatite (HA) into the matrix of collagen (Col) and chitosan (Chi) by in situ synthesis was introduced to prepare nanocomposites. Structural investigations of the pure Col-Chi mixture validated the influence of Chi on Col assembly, but the molecular interactions between Col and Chi was partially depressed during the intervention of in situ HA synthesis, as revealed by FTIR and DSC analyses. A series of Col-Chi-HA (CCHA) nanocomposites with varying HA content were thereby prepared by a sequential method, involving in situ synthesis in the Col-Chi system, then gelling at 25 degrees C and subsequently washing the resultant elastic gel followed by dehydration consolidation. The structural characteristics and biological properties of the dehydrated CCHA nanocomposites were further evaluated by using XRD, FTIR, TG, and SEM analyses and the osteoblast culture experiment. Formation of a well integrated microstructure of organic fibers (ca. 90 nm in size) and dense matrix including inorganic aggregates (less than 30 nm in size) was found in these nanocomposites. Rat Ros 17/2.8 Osteoblasts proliferated and attached well on the surface of both CCHA nanocomposite and Col-Chi mixture. These results indicated that in situ HA synthesis in the Col-Chi system provided a feasible route for bone grafting nanocomposites.

Biological evaluation of human hair keratin scaffolds for skin wound repair and regeneration

“…Collagen as one of the most widely used biologically-derived biomaterials has low its immunogenicity and good affinity to cells. Having been safely used in medical fields for decades, collagen-based biomaterials are now well documented in developing various ECM analogues [8,[12][13][14][15][16]. The wide use of other biologically-derived proteins for this purpose also includes fibrin [12], silk fibroin [17] and elastin [18].…”

Preparation and evaluation of biomimetric nano-hydroxyapatite-based composite scaffolds for bone-tissue engineering

“…A composite of HA and COL, the main components of bone, would be expected to have good bone-tissue reactions. Many researchers have therefore prepared HA/COL composites [4][5][6][7][8]. However, phosphatidylserine (PS) is an important component of cell velum lipid, and plays a key role in tissue development, repair, and function.…”

Design and Synthesis of Hydroxyapatite with Organic Modifiers for Application to Environmental Remediation

“…The chemical structure of organic modifiers affects HAP particle size [45]. After modifying HAP with Tween 20, there appeared to be small-sized HAP particles.…”