Dentinal tubule penetration of AH Plus, BC Sealer and a novel tricalcium silicate sealer: a confocal laser scanning microscopy study

Hachem, Roula El; Khalil, Issam; Brun, Guy Le; Pellen, Fabrice; Jeune, Bernard; Daou, Maha; Osta, Nada El; Naaman, Alfred; Abboud, Maher

doi:10.1007/s00784-018-2632-6

Cited by 63 publications

(96 citation statements)

References 36 publications

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“…Despite using human teeth extracted for different reasons (orthodontic or periodontal), the tubular penetration pattern followed the same trend in all teeth. Both for the depth of penetration inside the dentinal tubule and for the percentage of tubular penetration, the apical third showed the lowest results, followed by the middle and coronal thirds; coinciding with other authors [ 17 , 24 , 31 , 36 , 41 , 42 ]. This may be attributed to the differences in dentinal tubule distribution and caliber depending on the root third.…”

Section: Discussionsupporting

confidence: 89%

“…In relation to the results of the present study, ES showed a higher penetration compared to the other sealers although not significantly in all cases. This may be due, partially, to the size of the sealer particles (<1 μm) [ 34 ], which is considerably smaller than that of BR (5–30 μm) [ 35 ] and AHP (1.5–8 μm) [ 36 ]. In addition to its fluidity, this allows the sealer to reach greater depths within the dentinal tubules, with diameters between 2–3 μm [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

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Could the Calcium Silicate-Based Sealer Presentation Form Influence Dentinal Sealing? An In Vitro Confocal Laser Study on Tubular Penetration

et al. 2021

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Dentinal tubule penetration influences root canal treatment sealing. The aim of this study was to compare dentinal penetration of two clinical presentations of silicate-based sealers using confocal laser. Sixty single-rooted human teeth from 50–70 year-old patients extracted for orthodontic/periodontal reasons were used. Canals were prepared using Mtwo system up to 35/0.04, with 5.25% NaOCl irrigation and final irrigation using 17% EDTA. Teeth were randomly assigned into study groups (ni = 20): EndoSequence BC sealer (ES, group 1), BioRoot RCS (BR, group 2); and a control group (nc = 20) with AH Plus (AHP). Root canals were obturated with 35/0.04 gutta-percha (single-cone technique). The samples were obtained from apical, middle, and coronal thirds. Dentinal tubule penetration depth and percentage of penetration around the canal perimeter were measured. The statistical analysis was performed using Mann Whitney U test and Wilcoxon t-test (95% confidence interval). ES exhibited a significantly higher penetration than AHP in apical and middle thirds (p < 0.05), and in middle and coronal thirds relative to BR (p < 0.05). The percentage of penetration around the canal perimeter was significantly higher for ES compared to BR in all thirds, but only in the apical third for AHP (p < 0.05). The pre-mixed silicate-based sealer exhibited better penetration than the powder/liquid one.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Could the Calcium Silicate-Based Sealer Presentation Form Influence Dentinal Sealing? An In Vitro Confocal Laser Study on Tubular Penetration

et al. 2021

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“…Unlike scanning electron microscopy, confocal microscopy does not require vacuum or metallization, which is responsible for sample dehydration and the occurrence of technical artifacts 24 . Confocal microscopy has been widely used to observe and evaluate sealer penetration within the dentinal tubules 25 . In the present study, EDTA irrigation with Endovac significantly favored sealer penetration compared with other groups.…”

Section: Discussionmentioning

confidence: 66%

Comparative effects of final canal irrigation with chitosan and EDTA

et al. 2020

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Comparative effects of final canal irrigation with chitosan and EDTA Chitosan is a natural, biocompatible chelating substance with potential for dental use. This study compared the effects of final canal irrigation with chitosan and EDTA on dentin microhardness, sealer dentin tubules penetration capacity, and push-out strength. Methodology: Fifty canine roots were distributed according to the final irrigation protocol (n=10): G1-15% EDTA with conventional irrigation; G2-15% EDTA with Endovac; G3-0.2% chitosan with conventional irrigation; G4-0.2% chitosan with Endovac; and G5-without irrigation. Specimens were obturated (AH Plus) and sectioned in 3 slices per root third. The first slice was used for microhardness and sealer penetration assessments under a laser confocal microscope. The second was utilized in a push-out strength test. The third slice was discarded. Data were analyzed using 2-way ANOVA and Tukey's post hoc test (α<0.05). Failure mode was determined at x40 magnification. Results: Microhardness reduction was more significant in groups G2 and G4 (p<0.05). Sealer penetration through dentin was significantly greater in group G2 (p<0.05). There was no significant difference between groups G1, G3, and G4 (p>0.05). In general, all experimental groups presented similar bond resistance (p>0.05) that significantly differed from the control (p<0.001). Mixed type failures were predominant. Conclusions: In general, 0.2% chitosan and 15% EDTA solutions act in a similar manner with regard to the variables studied. The use of Endovac potentiates the effect of these solutions.

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“…On the other hand, the 1 mm level is chosen to see whether the sealer can seep into apical area between the flutes of instruments and canal walls and can penetrate into the dentine tubules. Several techniques, such as scanning electron microscopy, are available for evaluating the interface between dental material and dentine (Cervino et al, ; Giudice, Cicciù, Cervino, Lizio, & Visco, ; Balguerie, van der Sluis, Vallaeys, Gurgel‐Georgelin, & Diemer, ; Lo Giudice et al, ), light microscopy (Weis, Parashos, & Messer, ), and CLSM (Piai et al, ; El Hachem et al, ). CLSM was used in the present study due its advantages over other detection methods.…”

Section: Discussionmentioning

confidence: 99%

The effect of different obturation methods on sealer penetration alongside apically separated rotary nickel–titanium instruments: A confocal laser scanning microscopy study

Türker

Ozyurek

Tek

2020

Microscopy Res & Technique

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The effects of different obturation techniques on calcium silicate-based sealer penetration in the presence of apically separated rotary files were evaluated. Forty-eight extracted mandibular incisors were used. ProTaper F2 rotary files were separated at the apical thirds. Samples were divided into four groups (n = 12) according to obturation technique used: (a) cold lateral compaction (CLC); (b) single cone; (c) bulk-fill (BF) without a core material; and (d) thermoplastic injection (TI). Specimens were sectioned horizontally at 1 and 3 mm from the apex and studied using a confocal scanning laser microscope. The maximum tubule penetration depth and percentage of penetration were measured. Data were statistically analyzed using parametric and nonparametric tests with a significance level of 5%. Regarding penetration depth, a significant difference was found at 1 mm (p < .05), while no significant difference was found at 3 mm (p > .05). At the 3 mm level, all of the obturation techniques showed similar penetration depths. Regarding penetration percentage, the values of the CLC and TI groups were statistically less when compared with the BF group at 1 and 3 mm levels, respectively (p < .05). Under the limitations of this in vitro study, results suggest that the obturation technique may present a significant effect on sealer penetration. K E Y W O R D S confocal laser scanning microscope, obturation technique, sealer penetration, separated instruments

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Dentinal tubule penetration of AH Plus, BC Sealer and a novel tricalcium silicate sealer: a confocal laser scanning microscopy study

Abstract: Although no study has confirmed a relationship between the penetration depth of root canal sealers and the prevention of apical periodontitis, dentinal tubule sealer penetration may improve obturation quality.

Cited by 63 publications

References 36 publications

Could the Calcium Silicate-Based Sealer Presentation Form Influence Dentinal Sealing? An In Vitro Confocal Laser Study on Tubular Penetration

Could the Calcium Silicate-Based Sealer Presentation Form Influence Dentinal Sealing? An In Vitro Confocal Laser Study on Tubular Penetration

Comparative effects of final canal irrigation with chitosan and EDTA

The effect of different obturation methods on sealer penetration alongside apically separated rotary nickel–titanium instruments: A confocal laser scanning microscopy study

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