Dentin-Pulp Tissue Regeneration Approaches in Dentistry: An Overview and Current Trends

Şişmanoğlu, Soner; Ercal, Pınar

doi:10.1007/5584_2020_578

Cited by 16 publications

(15 citation statements)

References 159 publications

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“…The main idea behind RETs is to eliminate the disadvantages of the conventional endodontic treatments by restoring the normal physiological properties of the tooth, including tertiary dentinogenesis, dental proprioception and innate immunity to retain the natural dentition for a longer period of time. In order to achieve success in RETs, it is crucial to ensure the survival of stem cells while providing optimum antisepsis (1). The irrigants used in conventional endodontic treatments should contribute to debridement and have an antimicrobial activity and the ability to dissolve the necrotic tissues (2).…”

Section: Introductionmentioning

confidence: 99%

The cytotoxic effects of various endodontic irrigants on the viability of dental mesenchymal stem cells

Şişmanoğlu

Ercal

2021

Aust Endodontic J

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This study aimed to evaluate cytotoxic effects of various irrigation solutions used in regenerative endodontic treatments (RETs) on mesenchymal stem cells, and further examine the long-term effect of hypochlorous acid (HOCl) on the cell viability and alkaline phosphatase (ALP) activity. Stem cells were exposed to various concentrations of NaOCl, EDTA, chlorhexidine (CHX), etidronic acid (HEDP)/NaOCl combination and HOCl. HOCl was tested for its effects on ALP activity up to 21 days. Additionally, cell viability was measured fluorescently using calcein AM. The most cytotoxic irrigant was CHX even with the lowest concentration. NaOCl and HEDP/NaOCl with 1:100 dilution decreased viability to around 40%. HOCl showed the lowest cytotoxicity among all tested irrigants. HOCl also showed no significant reduction in ALP activity compared with the controls. The cytotoxicity of endodontic irrigants was time and concentration dependent. HOCl demonstrated promising results regarding viability and ALP activity, since RETs require host stem cell survival.

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Section: Introductionmentioning

confidence: 99%

The cytotoxic effects of various endodontic irrigants on the viability of dental mesenchymal stem cells

Şişmanoğlu

Ercal

2021

Aust Endodontic J

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“…TGF-β1 promotes tertiary dentinogenesis and primary odontoblastic differentiation. 78 It has been demonstrated that TGF-β1 stimulates the odontoblastic differentiation of DPSCs. The effect of TGF-β1 was synergistically enhanced by fibroblast growth fatocr-2 (FGF-2), as evidenced by the expression of DSP and DMP-1, elevated ALP activity, and the generation of mineralized nodules.…”

Section: Growth Factors In Dentin Regenerationmentioning

confidence: 99%

Dentin regeneration based on tooth tissue engineering: A review

Azaryan

Razavi

Hanafi‐Bojd

et al. 2023

Biotechnology Progress

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Missing or damaged teeth due to caries, genetic disorders, oral cancer, or infection may contribute to physical and mental impairment that reduces the quality of life.Despite major progress in dental tissue repair and those replacing missing teeth with prostheses, clinical treatments are not yet entirely satisfactory, as they do not regenerate tissues with natural teeth features. Therefore, much of the focus has centered on tissue engineering (TE) based on dental stem/progenitor cells to create bioengineered dental tissues. Many in vitro and in vivo studies have shown the use of cells in regenerating sections of a tooth or a whole tooth. Tooth tissue engineering (TTE), as a promising method for dental tissue regeneration, can form durable biological substitutes for soft and mineralized dental tissues. The cell-based TE approach, which directly seeds cells and bioactive components onto the biodegradable scaffolds, is currently the most potential method. Three essential components of this strategy are cells, scaffolds, and growth factors (GFs). This study investigates dentin regeneration after an injury such as caries using TE and stem/progenitor cell-based strategies. We begin by discussing about the biological structure of a dentin and dentinogenesis.The engineering of teeth requires knowledge of the processes that underlie the growth of an organ or tissue. Then, the three fundamental requirements for dentin regeneration, namely cell sources, GFs, and scaffolds are covered in the current study, which may ultimately lead to new insights in this field.

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“…GFs activate multiple signalling pathways and mechanisms that regulate the behaviour of SCs by binding to cell surface receptors [ 47 ]. BMP, TGF-β1, FGF, PDGF-BB, and IGF-1 among others are key GFs involved in DPC regeneration [ 48 ]; given their presence in CGF, 10 studies have investigated the effect of CGF on SCs in vitro in order to evaluate its potential to induce DPC regeneration (Fig. 2 ).…”

Section: Effects Of Cgf On Scs In Dpc Regenerationmentioning

confidence: 99%

The effects and potential applications of concentrated growth factor in dentin–pulp complex regeneration

Liu

Wang

et al. 2021

Stem Cell Res Ther

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The dentin–pulp complex is essential for the long-term integrity and viability of teeth but it is susceptible to damage caused by external factors. Because traditional approaches for preserving the dentin–pulp complex have various limitations, there is a need for novel methods for dentin–pulp complex reconstruction. The development of stem cell-based tissue engineering has given rise to the possibility of combining dental stem cells with a tissue-reparative microenvironment to promote dentin–pulp complex regeneration. Concentrated growth factor, a platelet concentrate, is a promising scaffold for the treatment of dentin–pulp complex disorders. Given its characteristics of autogenesis, convenience, usability, and biodegradability, concentrated growth factor has gained popularity in medical and dental fields for repairing bone defects and promoting soft-tissue healing. Numerous in vitro studies have demonstrated that concentrated growth factor can promote the proliferation and migration of dental stem cells. Here, we review the current state of knowledge on the effects of concentrated growth factor on stem cells and its potential applications in dentin–pulp complex regeneration.

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Dentin-Pulp Tissue Regeneration Approaches in Dentistry: An Overview and Current Trends

Cited by 16 publications

References 159 publications

The cytotoxic effects of various endodontic irrigants on the viability of dental mesenchymal stem cells

The cytotoxic effects of various endodontic irrigants on the viability of dental mesenchymal stem cells

Dentin regeneration based on tooth tissue engineering: A review

The effects and potential applications of concentrated growth factor in dentin–pulp complex regeneration

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