The cytotoxic effects of various endodontic irrigants on the viability of dental mesenchymal stem cells

Şişmanoğlu, Soner; Ercal, Pınar

doi:10.1111/aej.12570

Cited by 12 publications

(13 citation statements)

References 30 publications

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“…But this should be also related to other antiseptics commonly used in dental therapy. Low concentrated NaOCl (0.05%) did not affect dental mesenchymal stem cell survival after 10 min, whereas low concentrated CHX (0.02%) caused a strong cytotoxicity [ 32 ]. Further, computational prediction did not find any mutagenic, tumorigenic irritant and reproductive toxicity [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of sodium hypochlorite gel on bacteria associated with periodontal disease

Irani,

Jungbauer,

Sculean

et al. 2024

Clin Oral Invest

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Objectives An adjunct in non-surgical periodontal therapy might be sodium hypochlorite (NaOCl)–based agents. The purpose of the present in vitro study was to get deeper knowledge on the influence of different parameters as time after mixing, pH, and chemical composition of an amino acid 0.475% NaOCl (AA-NaOCl) gel consisting of two components on its anti-biofilm activity. Materials and methods Six-species biofilms were cultured for 5 days, before AA-NaOCl gel was applied. In the different series, the influence of the time after mixing of the two components before application, of the concentration of NaOCl in the gel mixture, of the pH of the gel mixture, and of an exchange of the amino acid component by hyaluronic acid (HA), was analyzed. Results Mixing time point experiments showed that the AA-NaOCl gel is capable of statistically significantly reducing colony-forming unit (cfu) counts up to 30 min after mixing, but only up to 20 min after mixing the reduction was more than 2 log10 cfu. The pH experiments indicate that a reduced pH results in a reduced activity of the NaOCl formulation. NaOCl concentrations in the formulation in the range from 0.475 to 0.2% provide adequate activity on biofilms. A HA/NaOCl gel was equally active against the biofilm as the AA-NaOCl gel. Conclusion Mixing of the components should be made in a timeframe of 20 min before applications. An optimization of the composition of the NaOCl formulation might be possible and should be a topic in further in vitro studies. Clinical relevance The AA-NaOCl gel formulation can be mixed up to 20 min before application. Further, the study indicates that the composition of the NaOCl gel formulation can be optimized.

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Section: Discussionmentioning

confidence: 99%

Effect of sodium hypochlorite gel on bacteria associated with periodontal disease

Irani,

Jungbauer,

Sculean

et al. 2024

Clin Oral Invest

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“…As a strong oxidizing agent, NaOCl is cytotoxic to the periapical tissue and stem cells, especially in case of regenerative endodontic treatment [199]. The cytotoxic effect to dental stem cells was reported to be concentration dependent [115,200]. Furthermore, Martin et al [201] found that dentin conditioning with 1.5% NaOCl resulted in greater survival and differentiation of stem cells of apical papilla compared to the 3% counterpart, whereas 6% NaOCl leads to lack of survival and differentiation.…”

Section: Side Effectmentioning

confidence: 99%

Advances in the Role of Sodium Hypochlorite Irrigant in Chemical Preparation of Root Canal Treatment

Cai

Chen

et al. 2023

BioMed Research International

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Irrigation of root canal system is of great significance to the success of endodontic treatment, where sodium hypochlorite (NaOCl) is the most widely used irrigant in chemical preparation. NaOCl functions by eliminating bacterial biofilms and dissolving organic tissue, which may vary according to several factors such as the microbiology of root canal infection and the concentration of the irrigant. It has been proposed that the effectiveness of NaOCl could be enhanced via several methods, including heating the irrigant, applying in conjunction with certain reagents, or activating by agitation techniques. Despite its antibacterial and tissue-dissolving capacities, NaOCl should be used with caution to avoid detrimental effect due to its cytotoxicity and negative effect on dentin properties. In this narrative review, we discussed the factors that affect the properties of NaOCl, the methods to improve its efficacy, and the side effects that might occur in clinical practice.

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“…Moreover, natural molecules are used in oral environment such as polyphenols, which have biological benefits as they are natural antibacterial and antioxidant molecules ( 95 ). These different materials are made commercially available in dental market and used worldwide even though they sometimes display cytotoxicity ( 96 ).…”

Section: The Involvement Of the Amps Released From The Adrenal Medull...mentioning

confidence: 99%

The antimicrobial peptides secreted by the chromaffin cells of the adrenal medulla link the neuroendocrine and immune systems: From basic to clinical studies

et al. 2022

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The increasing resistance to antibiotic treatments highlights the need for the development of new antimicrobial agents. Antimicrobial peptides (AMPs) have been studied to be used in clinical settings for the treatment of infections. Endogenous AMPs represent the first line defense of the innate immune system against pathogens; they also positively interfere with infection-associated inflammation. Interestingly, AMPs influence numerous biological processes, such as the regulation of the microbiota, wound healing, the induction of adaptive immunity, the regulation of inflammation, and finally express anti-cancer and cytotoxic properties. Numerous peptides identified in chromaffin secretory granules from the adrenal medulla possess antimicrobial activity: they are released by chromaffin cells during stress situations by exocytosis via the activation of the hypothalamo-pituitary axis. The objective of the present review is to develop complete informations including (i) the biological characteristics of the AMPs produced after the natural processing of chromogranins A and B, proenkephalin-A and free ubiquitin, (ii) the design of innovative materials and (iii) the involvement of these AMPs in human diseases. Some peptides are elective biomarkers for critical care medicine, may play an important role in the protection of infections (alone, or in combination with others or antibiotics), in the prevention of nosocomial infections, in the regulation of intestinal mucosal dynamics and of inflammation. They could play an important role for medical implant functionalization, such as catheters, tracheal tubes or oral surgical devices, in order to prevent infections after implantation and to promote the healing of tissues.

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The cytotoxic effects of various endodontic irrigants on the viability of dental mesenchymal stem cells

Cited by 12 publications

References 30 publications

Effect of sodium hypochlorite gel on bacteria associated with periodontal disease

Effect of sodium hypochlorite gel on bacteria associated with periodontal disease

Advances in the Role of Sodium Hypochlorite Irrigant in Chemical Preparation of Root Canal Treatment

The antimicrobial peptides secreted by the chromaffin cells of the adrenal medulla link the neuroendocrine and immune systems: From basic to clinical studies

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