Dental Location of Streptococcus mutans and Streptococcus sobrinus in Human: Harboring Both Species

Lindquist, Bertil; Emilson, Claes‐Göran

doi:10.1159/000261358

Cited by 34 publications

(27 citation statements)

References 17 publications

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“…In this context the increased percentage of S. sobrinus at sub-site B is in line with the results reported by Wennerholm and Emilson [1995]. Similarly, the positive association between S. mutans and S. sobrinus (table 3) was in agreement with the findings of Lindquist and Emilson [1991a].…”

Section: Discussionsupporting

confidence: 90%

Ecological Study of Streptococcus mutans, Streptococcus sobrinus and Lactobacillus spp. at Sub-Sites from Approximal Dental Plaque from Children

et al. 1997

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Previous immunofluorescence (IF) studies have indicated that Streptococcus mutans may preferentially colonise specific sub-sites within approximal plaque. The present study aimed to extend these observations to other mutans streptococci and lactobacilli in such gingival margin plaque. Two hundred and seventy approximal plaque samples were taken from 90 teeth (3 from each tooth) in 64 children; three gingival margin sub-sites in relation to the contact area: away from (A), to the side of (S) and below (B) the contact area. Samples were processed by indirect IF using high-titred polyclonal anti-S. mutans ‘c’, anti-S. sobrinus ‘d’, anti-L. casei and anti-L. acidophilus antisera. An overall positive association was found between S. mutans ‘c’ and S. sobrinus ‘d’ (p < 0.001). Significant differences (p < 0.1) were found between the proportional counts at each sub-site for S. mutans ‘c’: A = 39%, S = 51% and B = 70%, and for S. sobrinus ‘d’ 21, 33 and 49%. Mutans streptococci (MS) appeared to preferentially colonise the sub-site below the contact area (B = 81%), compared with sub-sites A and S (48 and 62%, respectively). S. mutans ‘c’ and S. sobrinus ‘d’ were detected together at sub-sites A = 12%, S = 22%, and B = 38%, with proportional counts at B sites being higher than those at A (B > A, p < 0.01, and B > S, p < 0.05). Lactobacillus spp. were isolated rarely, and were usually found together with MS. There was a positive relationship between the presence of lactobacilli or MS and caries (white spot lesions only), although these species could frequently be isolated from non-carious sites. The presence of both S. mutans ‘c’ and S. sobrinus ‘d’ were strongly correlated with early caries lesions. In addition, this study confirmed the variation in the microflora at different sub-sites within approximal dental plaque.

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Section: Discussionsupporting

confidence: 90%

Ecological Study of Streptococcus mutans, Streptococcus sobrinus and Lactobacillus spp. at Sub-Sites from Approximal Dental Plaque from Children

et al. 1997

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“…The beginning and development of caries are determined by a host-parasite relationship, which can be altered by several environmental factors as demonstrated by in vitro models simulating the in vivo phenomenon in the mouth (9,20). The in vitro studies of the bacterial relationship based on the action of these microorganisms in the plaque formation on tooth surfaces and their potential to induce caries are in agreement with several results reported in the literature (3,(8)(9)(10)14,15).…”

Section: Introductionsupporting

confidence: 85%

In vitro bacterial plaque suppression and recolonization by S. mutans and S. sobrinus

Pereira¹,

Gonçalves²,

Höfling³

2006

Braz. J. Microbiol.

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The in vitro study of the interactions between S. mutans and S. sobrinus is important to determine the role of these microorganisms in the formation of biofilms on dental structures and their potential to induce carious lesions. The objective of this research was to study the suppression of bacterial plaque formation and its recolonization by rifampycin-resistant S.mutans and streptomycin-resistant S. sobrinus. To study the competitive relationship between these species, previously standardized strains were incubated in media containing different fermentable carbohydrates. At determined time intervals, samples were collected from mixed cultures of S. mutans and S. sobrinus, diluted and plated on BHI-agar containing rifampycin or streptomycin to determine the number of viable cells of each species by counting colonyforming units. In order to study the bacterial colonization process and in vitro recolonization of bacterial plaque, three experiments were performed: I -co-cultivation of S. mutans and S. sobrinus; II -inoculation of bacterial plaque pre formed by S. sobrinus with S. mutans; and III -bacterial plaque pre formed by S. mutans dispersed and plated on BHI-agar containing streptomycin or rifampicin to determine the number of viable cells for each species. The results indicated a predominance of S. mutans in relation to S. sobrinus, demonstrating the capacity of S. mutans to inhibit plaque formation by S. sobrinus and recolonize the surfaces.

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“…Several studies have reported that children harboring both S. mutans and S. sobrinus had a significantly higher caries incidence than those with either S. mutans or S. sobrinus alone (16,17,19). Recently, a hypothesis stating that mixed colonization by both S. mutans and S. sobrinus is an important factor for the development of caries has been reported (20).…”

Section: Resultsmentioning

confidence: 99%

Detection of streptococcus mutans and streptococcus sobrinus in dental plaque samples from Brazilian preschool children by polymerase chain reaction

Franco¹,

Amoroso²,

Marin

et al. 2007

Braz. Dent. J.

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The purposes of this study were to detect S. mutans and S. sobrinus by polymerase chain reaction (PCR) amplification, and to relate their presence to the incidence of dental caries in 42 Brazilian preschool children. Dental plaque samples were collected from the cervical margin of all erupted teeth of 5-6 years old children with primary dentition, using a sterile explorer. Examination of the dmft (decayed, missing, filled teeth) index, performed following the World Health Organization (WHO) caries diagnostic criteria, showed a 2.71 score. Prevalence of S. mutans and S. sobrinus was respectively, of 85.7% and 14.3%; no dental plaque sample was either positive or negative for both bacterial species. Children harboring either S. mutans or S. sobrinus presented the same caries prevalence. PCR showed good discriminative ability for differentiation between these species, and suggested that it is a technique suitable for epidemiological studies on mutans streptococci.

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Dental Location of Streptococcus mutans and Streptococcus sobrinus in Human: Harboring Both Species

Cited by 34 publications

References 17 publications

Ecological Study of Streptococcus mutans, Streptococcus sobrinus and Lactobacillus spp. at Sub-Sites from Approximal Dental Plaque from Children

Ecological Study of Streptococcus mutans, Streptococcus sobrinus and Lactobacillus spp. at Sub-Sites from Approximal Dental Plaque from Children

In vitro bacterial plaque suppression and recolonization by S. mutans and S. sobrinus

Detection of streptococcus mutans and streptococcus sobrinus in dental plaque samples from Brazilian preschool children by polymerase chain reaction

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