Dental Implants Surface in vitro Decontamination Protocols

Batalha, Vanessa Coelho; Bueno, Raquel Abreu; Fronchetti, Edemar; Mariano, José Ricardo; Santin, Gabriela Cristina; Freitas, Karina Maria Salvatore de; Ortiz, Mariana Aparecida Lopes; Salmeron, Samira

doi:10.1055/s-0040-1721550

Cited by 11 publications

(13 citation statements)

References 36 publications

(70 reference statements)

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“…For this reason, in the last decades, the association of LLLT with photosensitizers has also been used to reduce or eliminate bacteria, in the called aPDT. 19,20,28 In this study, the aPDT protocol was the same used by Batalha et al 20 and similar results were obtained. These authors used the same sample of periodontal biofilm to contaminate dental implants in vitro and have a significant reduction in the number of CFUs compared with control group.…”

Section: Discussionsupporting

confidence: 83%

“…The preirradiation time of 1 minute was also defined based on literature. 19,20,28 Although the reduction in the number of CFUs for both cultures with both protocols was quite satisfactory, it has not yet been sufficient to eliminate microorganisms. This indicates that the association of physical and chemical methods, as suggested by Cai et al, 16 can be used for decontamination of dental implant surfaces.…”

Section: Discussionmentioning

confidence: 99%

“…The sample of periodontal biofilm was frozen and obtained from the sample of the Laboratory of Microbiology at Ingá University Center, Uningá. 20 It is a subgingival biofilm collected from a patient diagnosed with periodontitis. The S. aureus used was a standard strain (ATCC 25923) from the bacteria collection from the same laboratory at Ingá University Center, Uningá, which was frozen in Brain Heart Infusion (BHI) broth (Kasvi, São José dos Pinhais, Brazil) with 15% glycerol.…”

Section: Methodsmentioning

confidence: 99%

“…19 The use of lasers and antimicrobial photodynamic therapy (aPDT) for decontamination of the titanium implant surfaces has been studied with very promising results. 19,20 The great advantage of laser therapies is the absence of consequences seen in other methods such as local irritation and the development of bacterial resistance. 16 However, the effect of the laser alone as a decontaminant of implant surfaces is controversial topic in literature.…”

Section: Introductionmentioning

confidence: 99%

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Low-Level Laser and Antimicrobial Photodynamic Therapy Reduce Peri-implantitis–related Microorganisms Grown In Vitro

Tonin¹,

Brites²,

Mariano

et al. 2021

Eur J Dent

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Objective Currently, dental implants are a predictable treatment option for oral rehabilitation; however, complications such as peri-implant diseases are increasing every day. Thus, the aim of this study was to verify the efficacy, in vitro, of two protocols against cultures of periodontal biofilm and Staphylococcus aureus. Material and Methods Petri dishes for each of the following groups were used: control groups (C)—plates inoculated with periodontal biofilm (C.B; n = 4) or S. aureus (C.SA; n = 4) without any treatment; laser groups—plates inoculated with periodontal biofilm (low-level laser therapy [LLLT].B; n = 4) or S. aureus (LLLT.SA; n = 4) and treated with LLLT (660 nm, 30 mW, 50 J/cm2, and 47 seconds); antimicrobial photodynamic therapy groups (aPDT)—plates inoculated with periodontal biofilm (aPDT.B; n = 4) or S. aureus (aPDT.SA; n = 4) and treated with aPDT (red laser 660 nm, 30 mW, 50 J/cm2, 47 seconds + toluidine blue O (TBO) 100 µg/mL, and 1 minute). After treatments were performed, the contents of all plates were diluted and seeded for counting colony-forming units (CFUs). Statistical Analysis Results were analyzed with one-way analysis of variance (ANOVA), Tukey’s test, comparison of percentages, and independent t-tests with a 5% significance level. Results Both treatments, LLLT and aPDT, significantly reduced the number of CFUs for the two types of culture, LLLT.B (3.69 × 106 ± 0.20), aPDT.B (2.79 × 106 ± 0.13), LLLT.SA (4.10 × 106 ± 0.12), and aPDT.SA (3.23 × 106 ± 0.10) when compared with control groups C.B (5.18 × 106 ± 0.43) and C.SA (5.81 × 106 ± 0.16; p = 0.000). When treatment groups were compared separately, there was also a statistically significant difference (p = 0.000). None of the protocols were able to eliminate cultured microorganisms. Conclusion The LLLT and aPDT protocols effectively reduced cultures of periodontal biofilm and S. aureus in vitro, with the superiority of aPDT.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Low-Level Laser and Antimicrobial Photodynamic Therapy Reduce Peri-implantitis–related Microorganisms Grown In Vitro

Tonin¹,

Brites²,

Mariano

et al. 2021

Eur J Dent

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“…Depending on the type of peri-implant defect and the location of the implant, surgical treatment of peri-implantitis includes access surgery, resective surgery, peri-implant bone reconstruction (regenerative or reconstructive surgery), or a combination of these techniques [ 13 ]. Additionally, it is crucial to decontaminate the dental implant surface during nonsurgical or surgical procedures to stop the progression of the disease [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Mechanical Properties and Corrosion Behavior of Ti6Al4V Particles Obtained by Implantoplasty: An In Vitro Study. Part II

et al. 2021

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In the field of implant dentistry there are several mechanisms by which metal particles can be released into the peri-implant tissues, such as implant insertion, corrosion, wear, or surface decontamination techniques. The aim of this study was to evaluate the corrosion behavior of Ti6Al4V particles released during implantoplasty of dental implants treated due to periimplantitis. A standardized protocol was used to obtain metal particles produced during polishing the surface of Ti6Al4V dental implants. Physicochemical and biological characterization of the particles were described in Part I, while the mechanical properties and corrosion behavior have been studied in this study. Mechanical properties were determined by means of nanoindentation and X-ray diffraction. Corrosion resistance was evaluated by electrochemical testing in an artificial saliva medium. Corrosion parameters such as critical current density (icr), corrosion potential (ECORR), and passive current density (iCORR) have been determined. The samples for electrochemical behavior were discs of Ti6Al4V as-received and discs with the same mechanical properties and internal stresses than the particles from implantoplasty. The discs were cold-worked at 12.5% in order to achieve the same properties (hardness, strength, plastic strain, and residual stresses). The implantoplasty particles showed a higher hardness, strength, elastic modulus, and lower strain to fracture and a compressive residual stress. Resistance to corrosion of the implantoplasty particles decreased, and surface pitting was observed. This fact is due to the increase of the residual stress on the surfaces which favor the electrochemical reactions. The values of corrosion potential can be achieved in normal conditions and produce corroded debris which could be cytotoxic and cause tattooing in the soft tissues.

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Enhancing Titanium Disk Performance through In-Pack Cold Atmospheric Plasma Treatment

Martins,

da Silva Braz,

de Macedo

et al. 2024

ACS Biomater. Sci. Eng.

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While titanium dental implants have already been clinically established, ongoing research is continuously being conducted to advance the fields of osseointegration and bacterial resistance, seeking further improvements in these areas. In this study, we introduce an innovative method for treating titanium surfaces within tightly sealed packaging. Specifically, titanium discs, enclosed in surgical-grade packaging, underwent treatment using cold atmospheric plasma (CAP). The surfaces were thoroughly characterized in terms of wettability, crystalline structure, and chemical composition. Hemocompatibility analyses were conducted using blood diluted in sodium citrate (1:9) exposed to titanium discs for 30 min inside a CO 2 incubator at 37 °C. Subsequently, various blood parameters were evaluated, including prothrombin time (PT), activated partial thromboplastin time (APTT), and platelet adhesion. Microbiological analyses were also performed using Pseudomonas aeruginosa (ATCC 27853) for 4 h at 37 °C. The treatment with CAP Jet resulted in a reduction in contact angle without causing any changes in the crystalline structure. No statistically significant differences were observed in the blood parameters. The plasma-treated samples exhibited lower PT and APTT values compared to those of the control group. The surfaces treated with CAP Jet showed increased platelet activation, platelet density, and thrombus formation when compared with the untreated samples. Moreover, the treated surfaces demonstrated lower bacterial colony formation compared with other surfaces.

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Dental Implants Surface in vitro Decontamination Protocols

Cited by 11 publications

References 36 publications

Low-Level Laser and Antimicrobial Photodynamic Therapy Reduce Peri-implantitis–related Microorganisms Grown In Vitro

Low-Level Laser and Antimicrobial Photodynamic Therapy Reduce Peri-implantitis–related Microorganisms Grown In Vitro

Mechanical Properties and Corrosion Behavior of Ti6Al4V Particles Obtained by Implantoplasty: An In Vitro Study. Part II

Enhancing Titanium Disk Performance through In-Pack Cold Atmospheric Plasma Treatment

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