Background: The Greulich & Pyle (G&P) and Tanner & Whitehouse (TW) methods are frequently used to determine bone age. The question to be raised is, "Are these standards applicable to children of different ethnicity to those on which they are based?" Methods: Bone age was assessed using the G&P and TW3 methods, firstly by independent manual rating of 2 observers, followed by a single observer using the BoneXpert software programme. In total, 420 hand trauma radiographs for Saudi Arabians (220 males, 329 left, age range 1 to 18 years) performed in the period January 2012-September 2016 were assessed. Paired sample t test was used to compare the difference between mean bone age (BA) and mean chronological age (CA) and to compare the difference between manual and BoneXpert ratings. Statistical analysis was undertaken using SPSS v.25. Findings: We found a statistically significant difference between BA and CA in males when using G&P (mean difference-0.36 ± 1 years, p <0.01) and TW3 (mean difference-0.22 ±0.9 years, p=0.03) methods but not in females for either G&P (mean difference 0.13 ± 1.2 years) or TW3 (mean difference 0.08 ± 1.1 years). In males, BoneXpert results conformed to the manual ratings for TW3 but not for G&P, for which the mean difference between manual and BoneXpert ratings was-0.27 ±0.5 years (p<0.01). Interpretation: Our results indicate that manual and BoneXpert-derived G&P and TW3 bone age assessment can be applied with no modification to Saudi Arabian females. However, only TW3 BoneXpert-derived BA can be applied without caution to Saudi Arabian males.