Densified Electrochemical Sensors Based on Local Redox Cycling between Vertically Separated Electrodes in Substrate Generation/Chip Collection and Extended Feedback Modes

Ino, Kosuke; Kanno, Yusuke; Nishijo, Taku; Komaki, Hirokazu; Yamada, Yuta; Yoshida, Shinya; Takahashi, Yasufumi; Shiku, Hitoshi; Matsue, Tomokazu

doi:10.1021/ac500435d

Cited by 32 publications

(28 citation statements)

References 37 publications

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“…Finally, cell activity can be electrochemically detected by using marker proteins, such as endogenous alkaline phosphatase (ALP). For example, cell differentiation of embryonic stem cells and early‐stage bone differentiation have been electrochemically detected. Several types of integrated electrochemical devices have been developed for bioanalysis including cell analysis and they are discussed in detail elsewhere .…”

Section: Electrochemical Intracellular Sensing In Situmentioning

confidence: 99%

Intracellular Electrochemical Sensing

et al. 2018

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Observing biochemical processes within living cell is imperative for biological and medical research. Fluoresce imaging is widely used for intracellular sensing of cell membranes, nuclei, lysosomes, and pH. Electrochemical assays have been proposed as an alternative to fluorescence‐based assays because of excellent analytical features of electrochemical devices. Notably, thanks to the rapid progress of micro/nanotechnologies and electrochemical techniques, intracellular electrochemical sensing is making rapid progress, leading to a successful detection of intracellular components. Such insight can provide a deep understanding of cellular biological processes and, ultimately, define the human healthy and diseased states. In this review, we present an overview of recent research progress in intracellular electrochemical sensing. We focus on two main topics, electrochemical extraction of cytosolic contents from cells and intracellular electrochemical sensing in situ.

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Section: Electrochemical Intracellular Sensing In Situmentioning

confidence: 99%

Intracellular Electrochemical Sensing

et al. 2018

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“…The development of several types of electrochemical devices for bioassay applications, including analyses of yeast and mammalian cells, has been reported. [29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44] IDA electrodes were incorporated to amplify signals from cells and provide highly sensitive assays. Yeast cellbased IDA electrodes were utilized for the detection of hormone active chemicals.…”

Section: Chip-based Devices For Electrochemical Bioanalysismentioning

confidence: 99%

“…To overcome this difficulty, we proposed a novel electrochemical approach based on redox cycling, the local redox cycling-based electrochemical (LRC-EC) system. [33][34][35][36][37][38][39][40][41][42][43][44] In the LRC-EC system, two arrays of electrodes (n rows and n columns of electrodes) are orthogonally placed to produce n 2 crossing points (Fig. 4).…”

Section: Electrochemical Imaging and High-throughput Analysismentioning

confidence: 99%

“…In addition, vertically separated electrodes were incorporated into the LRC-EC device to further increase the sensor density. 33 These LRC-EC devices have been used in enzyme activity analyses, DNA arrays, and imaging of droplet-containing biosamples. Furthermore, the LRC-EC system has been used to detect enzymes secreted from single cells.…”

Section: Electrochemical Imaging and High-throughput Analysismentioning

confidence: 99%

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Microchemistry- and MEMS-based Integrated Electrochemical Devices for Bioassay Applications

Ino

2015

Electrochemistry

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Here, we present an overview of our recent research progress in development of electrochemical devices/systems for bioassays. These devices/systems are based on microchemistry and micro-electromechanical systems (MEMS) for sophisticated analytical and electrochemical measurements. In particular, we exploit the unique chemical reactions occurring in micrometer-size spaces and/or involving micrometer-size structures for bioassays, including cell analyses. This paper addresses five topics: probe-, chip-, large-scale-integration chip-, and dielectrophoresisbased devices, and electrodeposition of hydrogels for bioassays.

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“…Addressable electrochemical electrode arrays on a glass substrate have been reported where row and column electrodes are patterned in an interdigitated array to allow redox cycling to be activated at individually addressed sensing elements 22, 23 . This device appears to be limited to applications using redox cycling.…”

Section: Introductionmentioning

confidence: 99%

Two approaches for addressing electrochemical electrode arrays with reduced external connections

2015

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Although patterning hundreds or thousands of electrochemical electrodes on lab-on-a-chip devices is straightforward and cost-effective using photolithography, easily making connections between hundreds of electrodes and external amplifiers remains a bottleneck. Here we describe two electrode addressing approaches using multiple fluid compartments that can potentially reduce the number of external connections by ~100-fold. The first approach enables all compartments on the device to be filled with solution at the same time, and then each fluid compartment is sequentially electrically activated to make the measurements. The second approach achieves lower measurement noise by sequentially filling recording chambers with solution. We propose an equivalent circuit to explain measurement noise in these recording configurations and demonstrate application of the approaches to measure quantal exocytosis from individual cells. A principle advantage of using these approaches is that they reduce the fraction of the microchip area that needs to be dedicated to making external connections and therefore reduces the cost per working electrode.

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Densified Electrochemical Sensors Based on Local Redox Cycling between Vertically Separated Electrodes in Substrate Generation/Chip Collection and Extended Feedback Modes

Cited by 32 publications

References 37 publications

Intracellular Electrochemical Sensing

Intracellular Electrochemical Sensing

Microchemistry- and MEMS-based Integrated Electrochemical Devices for Bioassay Applications

Two approaches for addressing electrochemical electrode arrays with reduced external connections

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