Dengue Seroprevalence of Healthy Adults in Singapore: Serosurvey Among Blood Donors, 2009

Low, Swee Ling; Lam, Sally; Wong, Wing-Yan; Teo, Diana; Ng, Lee Ching; Tan, Li-Kiang

doi:10.4269/ajtmh.14-0671

Cited by 77 publications

(92 citation statements)

References 27 publications

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“…Since 2009, the number of reported dengue cases declined in Bangladesh [23]; however, as discussed by Sharmin et al [22], this was likely an artifact of changes in reporting criteria (i.e., laboratory confirmation was required in order to confirm cases instead of using a clinical diagnosis). As a result, the ‘tip of the iceberg’ analogy of dengue reporting is likely in play in Bangladesh, as it has been well-documented that passive surveillance involving case notifications does not accurately reflect the burden of dengue in such a geographical location [24]. …”

Section: Introductionmentioning

confidence: 99%

Dengue seroprevalence, seroconversion and risk factors in Dhaka, Bangladesh

Dhar-Chowdhury¹,

Paul²,

Haque³

et al. 2017

PLoS Negl Trop Dis

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BackgroundDengue virus (DENV) activity has been reported in Dhaka, Bangladesh since the early 1960s with the greatest burden of dengue fever and dengue hemorrhagic fever cases observed in 2000. Since this time, the intensity of dengue activity has varied from year to year, and its determining factors remained relatively unknown. In light of such gaps in knowledge, the main objectives of this study were to determine the magnitude of seroprevalence and seroconversion among the surveyed population, and establish the individual/household level risk factors for the presence of DENV antibodies among all age groups of target populations in the city of Dhaka.Methodology/Principal findingsConsidering the lack of fine scale investigations on the factors driving dengue activity in Bangladesh, a prospective cohort study involving serological surveys was undertaken with participant interviews and blood donation across the city of Dhaka in 2012. Study participants were recruited from 12 of 90 wards and blood samples were collected during both the pre-monsoon (n = 1125) and post-monsoon (n = 600) seasons of 2012. The findings revealed that the seroprevalence in all pre-monsoon samples was 80.0% (900/1125) while the seropositivity in the pre-monsoon samples that had paired post-monsoon samples was 83.3% (503/600). Of the 97 paired samples that were negative at the pre-monsoon time point, 56 were positive at the post-monsoon time point. This resulted in a seroprevalence of 93.2% (559/600) among individuals tested during the post-monsoon period. Seroprevalence trended higher with age with children exhibiting a lower seropositivity as compared to adults. Results from this study also indicated that DENV strains were the only flaviviruses circulating in Dhaka in 2012. A multivariate analysis revealed that age, possession of indoor potted plants, and types of mosquito control measures were significant factors associated with DENV seroprevalence; while attendance in public/mass gatherings, and use of mosquito control measures were significantly associated with DENV seroconversion after adjusting for all other variables.Conclusions/SignificanceOur study suggests that there is a high level of endemic dengue virus circulation in the city of Dhaka which has resulted in significant DENV seroprevalence among its residents. Seropositivity increased with age, however, a substantial proportion of children are at risk for DENV infections. Our serological analysis also documents considerable DENV seroconversion among study participants which indicates that a large proportion of the population in the city of Dhaka were newly exposed to DENV during the study period (pre-and post-monsoon 2012). High levels of seroconversion suggest that there was an intense circulation of DENV in 2012 and this may have resulted in a significant risk for viral associated illness. Findings of our study further indicated that home-based interventions, such as removing indoor potted plants and increased bed net use, in addition to vector control measures in public parks...

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Section: Introductionmentioning

confidence: 99%

Dengue seroprevalence, seroconversion and risk factors in Dhaka, Bangladesh

Dhar-Chowdhury¹,

Paul²,

Haque³

et al. 2017

PLoS Negl Trop Dis

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“…6 It affects more than 2.5 billion people annually and 975 million belonging to tropical and subtropical countries in Southeast Asia, the Pacific and the Americas with Africa bearing the major brunt of the disease amounting to 900 million cases annually. [7][8][9] It is estimated that worldwide nearly 2.5 billion people continue to live at risk of contracting the infection while 50 million cases and 24,000 deaths tend to occur in 100 endemic countries. Risk of mortality in treated cases of DHF/DSS is 1% while mortality rate among untreated cases escalates to 20%.…”

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confidence: 99%

Socio demographic and clinical profile of dengue fever cases at a tertiary care hospital, Hyderabad, Telangana

Chajhlana

Mahabhasyam

Varaprasada

et al. 2017

Int J Community Med Public Health

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Background: Dengue fever is caused by Dengue Virus, belonging to the family Flaviviridae, transmitted by Aedes aegypti and Aedes albopictus mosquito. There are four distinct dengue virus serotypes (DEN-1, DEN-2, DEN-3, and DEN-4). This viral disease of humans has become a major international public health concern in recent years. Differential diagnosis based on symptoms is challenging due to dengue’s non-specific symptoms such as fever, aches and fatigue that are often overlap with other endemic infections. Dengue-associated mortality can be reduced from 20–30% in severe cases to less than 1% with appropriate fluid replacement and supportive care, which is greatly facilitated by early diagnosis. Aim and objective: To study the demographic factors and clinical features of dengue fever cases in urban areas of Hyderabad.Methods: The present study was conducted by Department of Community Medicine in collaboration with Department of Microbiology, Kamineni academy of medical sciences and research center, Hyderabad. Study subjects: “Probable” dengue cases admitted in the various departments during July –December 2016. Data collection was done using a structured questionnaire. Serum samples were tested for NS1 antigen, IgM, IgG antibodies by rapid visual immunochromatography.Results: Out of 137 samples of clinically suspected dengue cases, 119 were found positive for dengue infection. Fever was the most common symptom found in all the patients Majority of cases, 56.3% were males. Thrombocytopenia, leucopenia and bleeding manifestation were found in 84.0 %, 84.8% and 58.8% patients respectively.Conclusions: Fever was the most common symptom found in all the patients. Majority of cases showed thrombocytopenia. Significant difference was found in the clinical signs and symptoms like fever, myalgia, hypotension, thrombocytopenia and leucopenia in dengue cases than controls.

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“…These sera contained neutralizing antibodies to DENV 1–4 (> 1:1000, as determined by an in-house cell-based plaque-reduction neutralization technique [PRNT]). ( 20 ) These samples also tested positive for the presence of dengue IgM and IgG antibodies using DENV commercial assays (Bioline Dengue Duo [Standard Diagnostics Inc.], Dengue Virus IgM Capture DxSelect [Focus Diagnostics, Cypress, CA, USA], and Panbio Dengue IgG Capture and IgG Indirect ELISA [Alere Inc.]). Samples B2015-S02 and B2015-S03 were designated as high IgM (> 72 Panbio units by IgM capture; positive is > 11) and high IgG (> 97 Panbio units by IgG capture; positive is > 22), while samples B2015-S04 and B2015-S05 were designated as low IgM (18 Panbio units by IgM capture; positive is > 11) and high IgG (> 87 Panbio units by IgG capture; positive is > 22).…”

Section: Methodsmentioning

confidence: 99%

External quality assessment of dengue and chikungunya diagnostics in the Asia Pacific region, 2015

Soh

Squires

Tan

et al. 2016

WPSAR

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ObjectiveTo conduct an external quality assessment (EQA) of dengue and chikungunya diagnostics among national-level public health laboratories in the Asia Pacific region following the first round of EQA for dengue diagnostics in 2013.MethodsTwenty-four national-level public health laboratories performed routine diagnostic assays on a proficiency testing panel consisting of two modules. Module A contained serum samples spiked with cultured dengue virus (DENV) or chikungunya virus (CHIKV) for the detection of nucleic acid and DENV non-structural protein 1 (NS1) antigen. Module B contained human serum samples for the detection of anti-DENV antibodies.ResultsAmong 20 laboratories testing Module A, 17 (85%) correctly detected DENV RNA by reverse transcription polymerase chain reaction (RT–PCR), 18 (90%) correctly determined serotype and 19 (95%) correctly identified CHIKV by RT–PCR. Ten of 15 (66.7%) laboratories performing NS1 antigen assays obtained the correct results. In Module B, 18/23 (78.3%) and 20/20 (100%) of laboratories correctly detected anti-DENV IgM and IgG, respectively. Detection of acute/recent DENV infection by both molecular (RT–PCR) and serological methods (IgM) was available in 19/24 (79.2%) participating laboratories.DiscussionAccurate laboratory testing is a critical component of dengue and chikungunya surveillance and control. This second round of EQA reveals good proficiency in molecular and serological diagnostics of these diseases in the Asia Pacific region. Further comprehensive diagnostic testing, including testing for Zika virus, should comprise future iterations of the EQA.

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Dengue Seroprevalence of Healthy Adults in Singapore: Serosurvey Among Blood Donors, 2009

Cited by 77 publications

References 27 publications

Dengue seroprevalence, seroconversion and risk factors in Dhaka, Bangladesh

Dengue seroprevalence, seroconversion and risk factors in Dhaka, Bangladesh

Socio demographic and clinical profile of dengue fever cases at a tertiary care hospital, Hyderabad, Telangana

External quality assessment of dengue and chikungunya diagnostics in the Asia Pacific region, 2015

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