Demonstration of a plasmonic thermocycler for the amplification of human androgen receptor DNA

Roche, Philip J. R.; Beitel, Lenore K.; Khan, Rifat; Lumbroso, Rose; Najih, Mohamed; Cheung, M.; Thiemann, Joachim; Veerasubramanian, Venkat; Trifiro, Mark; Chodavarapu, Vamsy P.; Kirk, Andrew G.

doi:10.1039/c2an35692a

Cited by 41 publications

(17 citation statements)

References 32 publications

(39 reference statements)

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“…More recently, plasmonics-generated localized heat, a process known as photothermal conversion, has been utilized to speed up the rate of PCR assay. The concept of using plasmonic heating for PCR amplification was first demonstrated in 2012 by Roche et al [120]. In their study, 60 nm citrate-capped Au NPs were added directly to the PCR reaction mixture to a final concentration of 6.6 pM ( Fig.…”

Section: Plasmonics-enhanced Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Plasmonic molecular assays: Recent advances and applications for mobile health

Wei

2018

Nano Res.

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Plasmonics-based biosensing assays have been extensively employed for biomedical applications. Significant advancements in use of plasmonic assays for the construction of point-of-care (POC) diagnostic methods have been made to provide effective and urgent health care of patients, especially in resourcelimited settings. This rapidly progressive research area, centered on the unique surface plasmon resonance (SPR) properties of metallic nanostructures with exceptional absorption and scattering abilities, has greatly facilitated the development of cost-effective, sensitive, and rapid strategies for disease diagnostics and improving patient healthcare in both developed and developing worlds. This review highlights the recent advances and applications of plasmonic technologies for highly sensitive protein and nucleic acid biomarker detection. In particular, we focus on the implementation and penetration of various plasmonic technologies in conventional molecular diagnostic assays, and discuss how such modification has resulted in simpler, faster, and more sensitive alternatives that are suited for point-of-use. Finally, integration of plasmonic molecular assays with various portable POC platforms for mobile health applications are highlighted.

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Section: Plasmonics-enhanced Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Plasmonic molecular assays: Recent advances and applications for mobile health

Wei

2018

Nano Res.

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“…The plasmonic amplification is carried out by photothermal heating of evenly dispersed gold nanorods (AuNRs) in PCR reaction by an 808nm vertical-cavity surface emitting laser (VCSEL) irradiation [13][14][15][16]. The real time detection is based on measuring transmitted 260nm power from a UV LED through PCR solution by a photodetector [17,18].…”

Section: Introductionmentioning

confidence: 99%

Model-based Analysis for Real-time Label-free Ultraviolet Quantification of Ultrafast Plasmonic Polymerase Chain Reaction

Mohammadyousef

Paliouras

Trifiro

et al. 2020

2020 42nd Annual International Conference of the IEEE Engineering in Medicine &Amp; Biology Society (EMBC)

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A mathematical model for DNA quantification was calibrated using experimental results from real-time 260nm absorption measurements of plasmonic PCR thermocycling. The effect of different PCR parameters on template amplification was investigated using the calibrated model. Clinical Relevance-The results will aid researchers to optimize PCR reagent concentrations and system conditions to achieve faster and more efficient amplification as well as more sensitive amplicon detection.

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“… 12 - 14 In addition, it has been shown that gold-based materials such as GNPs and gold thin films can be used as photothermal sources to achieve rapid thermal cycling for DNA amplification upon laser irradiation. 15 - 17 However, such approaches still require a separate cell lysis step to obtain genomic DNA prior to PCR thermocycling. 18 , 19 A potential solution to this limitation is provided by recently reported cell lysis methods based on the laser-induced photothermal effect of GNPs or gold nanorods (GNRs), which is capable of lysing bacterial cells through heat shock, thereby releasing genomic DNA for subsequent conventional PCR.…”

Section: Introductionmentioning

confidence: 99%

Gold Nanorod-based Photo-PCR System for One-Step, Rapid Detection of Bacteria

Kim

Park

et al. 2017

Nanotheranostics

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The polymerase chain reaction (PCR) has been an essential tool for diagnosis of infectious diseases, but conventional PCR still has some limitations with respect to applications to point-of-care (POC) diagnostic systems that require rapid detection and miniaturization. Here we report a light-based PCR method, termed as photo-PCR, which enables rapid detection of bacteria in a single step. In the photo-PCR system, poly(enthylene glycol)-modified gold nanorods (PEG-GNRs), used as a heat generator, are added into the PCR mixture, which is subsequently periodically irradiated with a 808-nm laser to create thermal cycling. Photo-PCR was able to significantly reduce overall thermal cycling time by integrating bacterial cell lysis and DNA amplification into a single step. Furthermore, when combined with KAPA2G fast polymerase and cooling system, the entire process of bacterial genomic DNA extraction and amplification was further shortened, highlighting the potential of photo-PCR for use in a portable, POC diagnostic system.

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Demonstration of a plasmonic thermocycler for the amplification of human androgen receptor DNA

Cited by 41 publications

References 32 publications

Plasmonic molecular assays: Recent advances and applications for mobile health

Plasmonic molecular assays: Recent advances and applications for mobile health

Model-based Analysis for Real-time Label-free Ultraviolet Quantification of Ultrafast Plasmonic Polymerase Chain Reaction

Gold Nanorod-based Photo-PCR System for One-Step, Rapid Detection of Bacteria

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